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- W4317936504 abstract "Abstract A type of protein/peptide pair known as Catcher/Tag pair spontaneously forms an intermolecular isopeptide bond which can be applied for biomolecular click reactions. Covalent protein conjugation using Catcher/Tag pairs has turned out to be a valuable tool in biotechnology and biomedicines, but it is essential to increase the current toolbox of orthogonal Catcher/Tag pairs to expand the range of applications further, for example, for controlled multiple‐fragment ligation. We report here the engineering of novel Catcher/Tag pairs for protein ligation, aided by a crystal structure of a minimal CnaB domain from Lactobacillus plantarum . We show that a newly engineered pair, called SilkCatcher/Tag enables efficient pH‐inducible protein ligation in addition to being compatible with the widely used SpyCatcher/Tag pair. Finally, we demonstrate the use of the SilkCatcher/Tag pair in the production of native‐sized highly repetitive spider‐silk‐like proteins with >90 % purity, which is not possible by traditional recombinant production methods." @default.
- W4317936504 created "2023-01-25" @default.
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- W4317936504 date "2023-02-07" @default.
- W4317936504 modified "2023-10-17" @default.
- W4317936504 title "Biomolecular Click Reactions Using a Minimal pH‐Activated Catcher/Tag Pair for Producing Native‐Sized Spider‐Silk Proteins**" @default.
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- W4317936504 doi "https://doi.org/10.1002/ange.202216371" @default.
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