Matches in SemOpenAlex for { <https://semopenalex.org/work/W4317939708> ?p ?o ?g. }
- W4317939708 abstract "The γ-tubulin ring complex (γ-TuRC) has essential roles in centrosomal and non-centrosomal microtubule organization during vertebrate mitosis. While there have been important advances in understanding γ-TuRC-dependent microtubule nucleation, γ-TuRC capping of microtubule minus-ends remains poorly characterized. Here, we utilized biochemical reconstitutions and cellular assays to characterize the human γ-TuRC’s capping activity. Single filament assays showed that the γ-TuRC remained associated with a nucleated microtubule for tens of minutes. In contrast, caps at dynamic microtubule minus-ends displayed lifetimes of ∼1 min. Reconstituted γ-TuRCs with nucleotide-binding deficient γ-tubulin (γ-tubulinΔGTP) formed ring-shaped complexes that did not nucleate microtubules but capped microtubule minus-ends with lifetimes similar to those measured for wild-type complexes. In dividing cells, microtubule regrowth assays revealed that while knockdown of γ-tubulin suppressed non-centrosomal microtubule formation, add-back of γ-tubulinΔGTP could substantially restore this process. Our results suggest that γ-TuRC capping is a nucleotide-binding-independent activity that plays a role in non-centrosomal microtubule organization during cell division." @default.
- W4317939708 created "2023-01-25" @default.
- W4317939708 creator A5011691413 @default.
- W4317939708 creator A5014265751 @default.
- W4317939708 creator A5017780275 @default.
- W4317939708 creator A5029772826 @default.
- W4317939708 creator A5031284852 @default.
- W4317939708 creator A5084513610 @default.
- W4317939708 date "2023-01-25" @default.
- W4317939708 modified "2023-10-06" @default.
- W4317939708 title "A nucleotide binding–independent role for γ-tubulin in microtubule capping and cell division" @default.
- W4317939708 cites W1518100516 @default.
- W4317939708 cites W1933219102 @default.
- W4317939708 cites W1974111066 @default.
- W4317939708 cites W1988573682 @default.
- W4317939708 cites W1990786456 @default.
- W4317939708 cites W2012083512 @default.
- W4317939708 cites W2015005644 @default.
- W4317939708 cites W2023488271 @default.
- W4317939708 cites W2023695022 @default.
- W4317939708 cites W2033018905 @default.
- W4317939708 cites W2038506832 @default.
- W4317939708 cites W2041589026 @default.
- W4317939708 cites W2058036222 @default.
- W4317939708 cites W2060582652 @default.
- W4317939708 cites W2071451843 @default.
- W4317939708 cites W2076659391 @default.
- W4317939708 cites W2082968313 @default.
- W4317939708 cites W2083833358 @default.
- W4317939708 cites W2093012794 @default.
- W4317939708 cites W2097313305 @default.
- W4317939708 cites W2113799806 @default.
- W4317939708 cites W2119471586 @default.
- W4317939708 cites W2131528707 @default.
- W4317939708 cites W2131938193 @default.
- W4317939708 cites W2132629607 @default.
- W4317939708 cites W2135241130 @default.
- W4317939708 cites W2136304736 @default.
- W4317939708 cites W2145264628 @default.
- W4317939708 cites W2146139221 @default.
- W4317939708 cites W2149596669 @default.
- W4317939708 cites W2152333325 @default.
- W4317939708 cites W2157253543 @default.
- W4317939708 cites W2160898059 @default.
- W4317939708 cites W2217038394 @default.
- W4317939708 cites W2334348545 @default.
- W4317939708 cites W2338404646 @default.
- W4317939708 cites W2522470354 @default.
- W4317939708 cites W2550353151 @default.
- W4317939708 cites W2585722462 @default.
- W4317939708 cites W2588526630 @default.
- W4317939708 cites W2608056363 @default.
- W4317939708 cites W2620662403 @default.
- W4317939708 cites W2734356283 @default.
- W4317939708 cites W2747750550 @default.
- W4317939708 cites W2789554578 @default.
- W4317939708 cites W2800250383 @default.
- W4317939708 cites W2945921566 @default.
- W4317939708 cites W2981610884 @default.
- W4317939708 cites W2990301359 @default.
- W4317939708 cites W2994978818 @default.
- W4317939708 cites W2995545804 @default.
- W4317939708 cites W3017081186 @default.
- W4317939708 cites W3028549436 @default.
- W4317939708 cites W3039974459 @default.
- W4317939708 cites W3103963484 @default.
- W4317939708 cites W3110769241 @default.
- W4317939708 cites W3121676293 @default.
- W4317939708 cites W3126642644 @default.
- W4317939708 cites W3158782292 @default.
- W4317939708 cites W3213934415 @default.
- W4317939708 cites W4210694026 @default.
- W4317939708 cites W4210715229 @default.
- W4317939708 cites W4225639657 @default.
- W4317939708 cites W4226164766 @default.
- W4317939708 doi "https://doi.org/10.1083/jcb.202204102" @default.
- W4317939708 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36695784" @default.
- W4317939708 hasPublicationYear "2023" @default.
- W4317939708 type Work @default.
- W4317939708 citedByCount "2" @default.
- W4317939708 countsByYear W43179397082023 @default.
- W4317939708 crossrefType "journal-article" @default.
- W4317939708 hasAuthorship W4317939708A5011691413 @default.
- W4317939708 hasAuthorship W4317939708A5014265751 @default.
- W4317939708 hasAuthorship W4317939708A5017780275 @default.
- W4317939708 hasAuthorship W4317939708A5029772826 @default.
- W4317939708 hasAuthorship W4317939708A5031284852 @default.
- W4317939708 hasAuthorship W4317939708A5084513610 @default.
- W4317939708 hasBestOaLocation W43179397081 @default.
- W4317939708 hasConcept C10447061 @default.
- W4317939708 hasConcept C118716 @default.
- W4317939708 hasConcept C12554922 @default.
- W4317939708 hasConcept C1491633281 @default.
- W4317939708 hasConcept C181199279 @default.
- W4317939708 hasConcept C20418707 @default.
- W4317939708 hasConcept C29537977 @default.
- W4317939708 hasConcept C55493867 @default.
- W4317939708 hasConcept C84425145 @default.
- W4317939708 hasConcept C85813293 @default.
- W4317939708 hasConcept C86803240 @default.