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- W4318070576 abstract "Unvaccinated individuals in endemic areas with proven enzootic transmission of Yellow fever virus are at risk of infection due to a dramatic shift in the epidemiology of the disease over recent years. For this reason, epidemiological surveillance and laboratory confirmation of cases have become mandatory.To develop and test a control RNA for YFV detection through real-time RT-PCR.A 437-bp insert containing the T7 promoter and the target sequences for two different in-house protocols was designed in the context of the pUC57 vector and obtained through gene synthesis. After T7-driven in vitro transcription, standard curves were developed for Log10 serial dilutions of the YFV control RNA with 8 replicates.A dynamic range of quantification of 10 orders of magnitude was observed with a limit of detection of 6.3 GCE/µL (95% CI, 2.6 to 139.4 GCE/µL).The plasmid construct is available for YFV molecular test validation on clinical, entomological, and epizootic samples." @default.
- W4318070576 created "2023-01-26" @default.
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- W4318070576 date "2023-04-01" @default.
- W4318070576 modified "2023-10-17" @default.
- W4318070576 title "Usefulness of an in vitro-transcribed RNA control for the detection and quantification of Yellow fever virus through real-time reverse transcription-polymerase chain reaction" @default.
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- W4318070576 doi "https://doi.org/10.1016/j.idnow.2023.104654" @default.
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