FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4318391794> ?p ?o ?g. }

• W4318391794 abstract "The preparation of Escherichia coli competent cells by calcium chloride is a common method in molecular biology, but the mechanism is poorly understood. In a previous study, using transcriptomics and proteomics approaches, we found that the expression pattern of the gene loiP was upregulated by CaCl2. In order to investigate the function of the loiP gene in Ca2+- mediated formation of competent cells of E. coli DH5α, the loiP gene deletion strains were constructed by the lambda-derived Red homologous recombination technique. Then, the cell morphology, transformation efficiency, and cell membrane changes of the competent cells of the mutant strain were further explored. Compared with the wild-type E. coli DH5α, the mutant strains have no significant differences in the morphology, growth characteristics, and the permeability of the intracellular membrane. However, the transformation efficiencies of the mutant strains to plasmids of different sizes were significantly reduced, and the permeability of the outer membrane decreased by 2.94 times. These results indicate that the deletion of gene loiP may directly affect the transformation efficiency and outer membrane permeability of E. coli competent cells." @default.
• W4318391794 created "2023-01-29" @default.
• W4318391794 creator A5006380836 @default.
• W4318391794 creator A5008437767 @default.
• W4318391794 creator A5011241874 @default.
• W4318391794 creator A5023117785 @default.
• W4318391794 creator A5024345025 @default.
• W4318391794 creator A5038814495 @default.
• W4318391794 creator A5057023277 @default.
• W4318391794 date "2023-01-27" @default.
• W4318391794 modified "2023-09-24" @default.
• W4318391794 title "The function of the gene <i>loiP</i> in transformation efficiency and outer membrane permeability change of <i>Escherichia coli</i> treated by Ca2+ ions" @default.
• W4318391794 cites W1954110755 @default.
• W4318391794 cites W1966256017 @default.
• W4318391794 cites W1967370316 @default.
• W4318391794 cites W1982354966 @default.
• W4318391794 cites W1986684101 @default.
• W4318391794 cites W1987296022 @default.
• W4318391794 cites W2000379854 @default.
• W4318391794 cites W2015002698 @default.
• W4318391794 cites W2026577011 @default.
• W4318391794 cites W2030768253 @default.
• W4318391794 cites W2043929154 @default.
• W4318391794 cites W2048729067 @default.
• W4318391794 cites W2061160519 @default.
• W4318391794 cites W2077897893 @default.
• W4318391794 cites W2078725488 @default.
• W4318391794 cites W2079427117 @default.
• W4318391794 cites W2081794308 @default.
• W4318391794 cites W2091927928 @default.
• W4318391794 cites W2102978118 @default.
• W4318391794 cites W2109331116 @default.
• W4318391794 cites W2120183465 @default.
• W4318391794 cites W2132065459 @default.
• W4318391794 cites W2136670217 @default.
• W4318391794 cites W2139216324 @default.
• W4318391794 cites W2190959760 @default.
• W4318391794 cites W2751109928 @default.
• W4318391794 doi "https://doi.org/10.1093/lambio/ovac076" @default.
• W4318391794 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36708165" @default.
• W4318391794 hasPublicationYear "2023" @default.
• W4318391794 type Work @default.
• W4318391794 citedByCount "0" @default.
• W4318391794 crossrefType "journal-article" @default.
• W4318391794 hasAuthorship W4318391794A5006380836 @default.
• W4318391794 hasAuthorship W4318391794A5008437767 @default.
• W4318391794 hasAuthorship W4318391794A5011241874 @default.
• W4318391794 hasAuthorship W4318391794A5023117785 @default.
• W4318391794 hasAuthorship W4318391794A5024345025 @default.
• W4318391794 hasAuthorship W4318391794A5038814495 @default.
• W4318391794 hasAuthorship W4318391794A5057023277 @default.
• W4318391794 hasBestOaLocation W43183917941 @default.
• W4318391794 hasConcept C102744134 @default.
• W4318391794 hasConcept C104317684 @default.
• W4318391794 hasConcept C143065580 @default.
• W4318391794 hasConcept C146587185 @default.
• W4318391794 hasConcept C1491633281 @default.
• W4318391794 hasConcept C153911025 @default.
• W4318391794 hasConcept C178050479 @default.
• W4318391794 hasConcept C204241405 @default.
• W4318391794 hasConcept C22744801 @default.
• W4318391794 hasConcept C2778106830 @default.
• W4318391794 hasConcept C2779656422 @default.
• W4318391794 hasConcept C2992270614 @default.
• W4318391794 hasConcept C41625074 @default.
• W4318391794 hasConcept C547475151 @default.
• W4318391794 hasConcept C55493867 @default.
• W4318391794 hasConcept C79879829 @default.
• W4318391794 hasConcept C86803240 @default.
• W4318391794 hasConcept C95444343 @default.
• W4318391794 hasConceptScore W4318391794C102744134 @default.
• W4318391794 hasConceptScore W4318391794C104317684 @default.
• W4318391794 hasConceptScore W4318391794C143065580 @default.
• W4318391794 hasConceptScore W4318391794C146587185 @default.
• W4318391794 hasConceptScore W4318391794C1491633281 @default.
• W4318391794 hasConceptScore W4318391794C153911025 @default.
• W4318391794 hasConceptScore W4318391794C178050479 @default.
• W4318391794 hasConceptScore W4318391794C204241405 @default.
• W4318391794 hasConceptScore W4318391794C22744801 @default.
• W4318391794 hasConceptScore W4318391794C2778106830 @default.
• W4318391794 hasConceptScore W4318391794C2779656422 @default.
• W4318391794 hasConceptScore W4318391794C2992270614 @default.
• W4318391794 hasConceptScore W4318391794C41625074 @default.
• W4318391794 hasConceptScore W4318391794C547475151 @default.
• W4318391794 hasConceptScore W4318391794C55493867 @default.
• W4318391794 hasConceptScore W4318391794C79879829 @default.
• W4318391794 hasConceptScore W4318391794C86803240 @default.
• W4318391794 hasConceptScore W4318391794C95444343 @default.
• W4318391794 hasFunder F4320321001 @default.
• W4318391794 hasFunder F4320325188 @default.
• W4318391794 hasIssue "2" @default.
• W4318391794 hasLocation W43183917941 @default.
• W4318391794 hasLocation W43183917942 @default.
• W4318391794 hasOpenAccess W4318391794 @default.
• W4318391794 hasPrimaryLocation W43183917941 @default.
• W4318391794 hasRelatedWork W1969844075 @default.
• W4318391794 hasRelatedWork W2122087710 @default.
• W4318391794 hasRelatedWork W2356594179 @default.
• W4318391794 hasRelatedWork W2358348811 @default.
• W4318391794 hasRelatedWork W2361405022 @default.

• next