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- W4318407569 abstract "Abstract Haploid embryos have contributed significantly to our understanding of the role of parental genomes in development and can be applied to important biotechnology for human and animal species. However, development to the blastocyst stage is severely hindered in bovine haploid androgenetic embryos (hAE). To further our understanding of such developmental arrest, we performed a comprehensive comparison of the transcriptomic profile of morula-stage embryos, which were validated by qRT-PCR of transcripts associated with differentiation in haploid and biparental embryos. Among numerous disturbances, results showed that pluripotency pathways, especially the wingless-related integration site (WNT) signaling, were particularly unbalanced in hAE. Moreover, transcript levels of KLF4, NANOG, POU5F1, SOX2, CDX2, CTNNBL1, AXIN2 , and GSK3B were noticeably altered in hAE, suggesting disturbance of pluripotency and canonical WNT pathway. To evaluate the role of WNT on hAE competence, we exposed early day-5 morula stage embryos to the GSK3B inhibitor CHIR99021. Although no alterations were observed in pluripotency and WNT-related transcripts, exposure to CHIR99021 improved their ability to reach the blastocysts stage, confirming the importance of the WNT pathway in the developmental features of bovine hAE. Summary statement This study shows the importance of the WNT pathway on bovine haploid androgenetic development by walking through transcriptomics and pluripotency markers associated with cell fate determination during early development." @default.
- W4318407569 created "2023-01-29" @default.
- W4318407569 creator A5001129646 @default.
- W4318407569 creator A5008036456 @default.
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- W4318407569 creator A5066092830 @default.
- W4318407569 creator A5074833069 @default.
- W4318407569 creator A5091323560 @default.
- W4318407569 date "2023-01-28" @default.
- W4318407569 modified "2023-10-04" @default.
- W4318407569 title "Haploid androgenetic development in bovines reveals imbalanced WNT signaling and impaired cell fate differentiation" @default.
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