Matches in SemOpenAlex for { <https://semopenalex.org/work/W4318476679> ?p ?o ?g. }
- W4318476679 endingPage "251" @default.
- W4318476679 startingPage "233" @default.
- W4318476679 abstract "Lipid droplets (LDs) are fat storage organelles highly abundant in oocytes and eggs of many vertebrates and invertebrates. They have roles both during oogenesis and in provisioning the developing embryo. In Drosophila, large numbers of LDs are generated in nurse cells during mid-oogenesis and then transferred to oocytes. Their number and spatial distribution changes developmentally and in response to various experimental manipulations. This chapter demonstrates how to visualize LDs in Drosophila follicles, both in fixed tissues and living samples. For fixed samples, the protocol explains how to prepare female flies, dissect ovaries, isolate follicles, fix, apply stains, mount the tissue, and perform imaging. For live samples, the protocol shows how to dissect ovaries, apply a fluorescent LD dye, and culture follicles such that they remain alive and healthy during imaging. Finally, a method is provided that employs in vivo centrifugation to assess colocalization of markers with LDs." @default.
- W4318476679 created "2023-01-30" @default.
- W4318476679 creator A5030134051 @default.
- W4318476679 creator A5060666860 @default.
- W4318476679 date "2023-01-01" @default.
- W4318476679 modified "2023-09-26" @default.
- W4318476679 title "Visualizing Lipid Droplets in Drosophila Oogenesis" @default.
- W4318476679 cites W1485927957 @default.
- W4318476679 cites W1785740069 @default.
- W4318476679 cites W1974077951 @default.
- W4318476679 cites W1987703403 @default.
- W4318476679 cites W1992609426 @default.
- W4318476679 cites W2001044160 @default.
- W4318476679 cites W2004655327 @default.
- W4318476679 cites W2013153343 @default.
- W4318476679 cites W2014186431 @default.
- W4318476679 cites W2030498778 @default.
- W4318476679 cites W2031299871 @default.
- W4318476679 cites W2038027899 @default.
- W4318476679 cites W2048473302 @default.
- W4318476679 cites W2049527119 @default.
- W4318476679 cites W2084177233 @default.
- W4318476679 cites W2092905869 @default.
- W4318476679 cites W2100253993 @default.
- W4318476679 cites W2100608670 @default.
- W4318476679 cites W2112520822 @default.
- W4318476679 cites W2121233961 @default.
- W4318476679 cites W2134579258 @default.
- W4318476679 cites W2139486492 @default.
- W4318476679 cites W2139865462 @default.
- W4318476679 cites W2140802932 @default.
- W4318476679 cites W2147026515 @default.
- W4318476679 cites W2160708064 @default.
- W4318476679 cites W2388541855 @default.
- W4318476679 cites W2585714766 @default.
- W4318476679 cites W2626003366 @default.
- W4318476679 cites W2739293386 @default.
- W4318476679 cites W2782945385 @default.
- W4318476679 cites W2884515763 @default.
- W4318476679 cites W2949107687 @default.
- W4318476679 cites W2995272797 @default.
- W4318476679 cites W3087689951 @default.
- W4318476679 cites W3170359599 @default.
- W4318476679 cites W3187060569 @default.
- W4318476679 cites W3193168628 @default.
- W4318476679 doi "https://doi.org/10.1007/978-1-0716-2970-3_12" @default.
- W4318476679 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36715908" @default.
- W4318476679 hasPublicationYear "2023" @default.
- W4318476679 type Work @default.
- W4318476679 citedByCount "1" @default.
- W4318476679 countsByYear W43184766792023 @default.
- W4318476679 crossrefType "book-chapter" @default.
- W4318476679 hasAuthorship W4318476679A5030134051 @default.
- W4318476679 hasAuthorship W4318476679A5060666860 @default.
- W4318476679 hasConcept C104317684 @default.
- W4318476679 hasConcept C1491633281 @default.
- W4318476679 hasConcept C15526883 @default.
- W4318476679 hasConcept C163949779 @default.
- W4318476679 hasConcept C168240541 @default.
- W4318476679 hasConcept C196843134 @default.
- W4318476679 hasConcept C25095133 @default.
- W4318476679 hasConcept C2776041557 @default.
- W4318476679 hasConcept C2776690073 @default.
- W4318476679 hasConcept C2780104201 @default.
- W4318476679 hasConcept C40209533 @default.
- W4318476679 hasConcept C519853106 @default.
- W4318476679 hasConcept C54355233 @default.
- W4318476679 hasConcept C86803240 @default.
- W4318476679 hasConcept C87073359 @default.
- W4318476679 hasConcept C95444343 @default.
- W4318476679 hasConceptScore W4318476679C104317684 @default.
- W4318476679 hasConceptScore W4318476679C1491633281 @default.
- W4318476679 hasConceptScore W4318476679C15526883 @default.
- W4318476679 hasConceptScore W4318476679C163949779 @default.
- W4318476679 hasConceptScore W4318476679C168240541 @default.
- W4318476679 hasConceptScore W4318476679C196843134 @default.
- W4318476679 hasConceptScore W4318476679C25095133 @default.
- W4318476679 hasConceptScore W4318476679C2776041557 @default.
- W4318476679 hasConceptScore W4318476679C2776690073 @default.
- W4318476679 hasConceptScore W4318476679C2780104201 @default.
- W4318476679 hasConceptScore W4318476679C40209533 @default.
- W4318476679 hasConceptScore W4318476679C519853106 @default.
- W4318476679 hasConceptScore W4318476679C54355233 @default.
- W4318476679 hasConceptScore W4318476679C86803240 @default.
- W4318476679 hasConceptScore W4318476679C87073359 @default.
- W4318476679 hasConceptScore W4318476679C95444343 @default.
- W4318476679 hasLocation W43184766791 @default.
- W4318476679 hasLocation W43184766792 @default.
- W4318476679 hasOpenAccess W4318476679 @default.
- W4318476679 hasPrimaryLocation W43184766791 @default.
- W4318476679 hasRelatedWork W1785740069 @default.
- W4318476679 hasRelatedWork W1966641900 @default.
- W4318476679 hasRelatedWork W1978979621 @default.
- W4318476679 hasRelatedWork W2026412213 @default.
- W4318476679 hasRelatedWork W2110874492 @default.
- W4318476679 hasRelatedWork W2409265747 @default.
- W4318476679 hasRelatedWork W2617507009 @default.
- W4318476679 hasRelatedWork W3134001852 @default.