Matches in SemOpenAlex for { <https://semopenalex.org/work/W4318755613> ?p ?o ?g. }
- W4318755613 endingPage "3568" @default.
- W4318755613 startingPage "3561" @default.
- W4318755613 abstract "During developmental processes and wound healing, activation of living cells occurs with spatiotemporal precision and leads to rapid release of soluble molecular signals, allowing communication and coordination between neighbors. Nonliving systems capable of similar responsive release hold great promise for information transfer in materials and site-specific drug delivery. One nonliving system that offers a tunable platform for programming release is synthetic cells. Encased in a lipid bilayer structure, synthetic cells can be outfitted with molecular conduits that span the bilayer and lead to material exchange. While previous work expressing membrane pore proteins in synthetic cells demonstrated content exchange, user-defined control over release has remained elusive. In mammalian cells, connexon nanopore structures drive content release and have garnered significant interest since they can direct material exchange through intercellular contacts. Here, we focus on connexon nanopores and present activated release of material from synthetic cells in a light-sensitive fashion. To do this, we re-engineer connexon nanopores to assemble after post-translational processing by a protease. By encapsulating proteases in light-sensitive liposomes, we show that assembly of nanopores can be triggered by illumination, resulting in rapid release of molecules encapsulated within synthetic cells. Controlling connexon nanopore activity provides an opportunity for initiating communication with extracellular signals and for transferring molecular agents to the cytoplasm of living cells in a rapid, light-guided manner." @default.
- W4318755613 created "2023-02-02" @default.
- W4318755613 creator A5047319105 @default.
- W4318755613 creator A5052724583 @default.
- W4318755613 creator A5064825362 @default.
- W4318755613 creator A5065692675 @default.
- W4318755613 date "2023-02-01" @default.
- W4318755613 modified "2023-10-14" @default.
- W4318755613 title "Light-Activated Assembly of Connexon Nanopores in Synthetic Cells" @default.
- W4318755613 cites W1016059894 @default.
- W4318755613 cites W1909254456 @default.
- W4318755613 cites W1965373677 @default.
- W4318755613 cites W1967139808 @default.
- W4318755613 cites W1985256248 @default.
- W4318755613 cites W1987445483 @default.
- W4318755613 cites W1989713239 @default.
- W4318755613 cites W1999242918 @default.
- W4318755613 cites W2013546443 @default.
- W4318755613 cites W2017203200 @default.
- W4318755613 cites W2030683115 @default.
- W4318755613 cites W2043688195 @default.
- W4318755613 cites W2054127621 @default.
- W4318755613 cites W2056289501 @default.
- W4318755613 cites W2062887543 @default.
- W4318755613 cites W2066102742 @default.
- W4318755613 cites W2073283522 @default.
- W4318755613 cites W2085008263 @default.
- W4318755613 cites W2086785381 @default.
- W4318755613 cites W2118450542 @default.
- W4318755613 cites W2120061656 @default.
- W4318755613 cites W2130166533 @default.
- W4318755613 cites W2146692959 @default.
- W4318755613 cites W2157630661 @default.
- W4318755613 cites W2161962584 @default.
- W4318755613 cites W2164982825 @default.
- W4318755613 cites W2169171478 @default.
- W4318755613 cites W2195753589 @default.
- W4318755613 cites W2245069706 @default.
- W4318755613 cites W2315384917 @default.
- W4318755613 cites W2321454478 @default.
- W4318755613 cites W2346325477 @default.
- W4318755613 cites W2461784184 @default.
- W4318755613 cites W2464564128 @default.
- W4318755613 cites W2513245519 @default.
- W4318755613 cites W2514462360 @default.
- W4318755613 cites W2523550430 @default.
- W4318755613 cites W2670284967 @default.
- W4318755613 cites W2731130386 @default.
- W4318755613 cites W2746429854 @default.
- W4318755613 cites W2750462076 @default.
- W4318755613 cites W2752290919 @default.
- W4318755613 cites W2765328469 @default.
- W4318755613 cites W2766858627 @default.
- W4318755613 cites W2767162991 @default.
- W4318755613 cites W2790686372 @default.
- W4318755613 cites W2793941104 @default.
- W4318755613 cites W2796153225 @default.
- W4318755613 cites W2804651513 @default.
- W4318755613 cites W2897575226 @default.
- W4318755613 cites W2901918409 @default.
- W4318755613 cites W2906825470 @default.
- W4318755613 cites W2942257291 @default.
- W4318755613 cites W2943065135 @default.
- W4318755613 cites W2952815301 @default.
- W4318755613 cites W2963025303 @default.
- W4318755613 cites W2970232210 @default.
- W4318755613 cites W2973793693 @default.
- W4318755613 cites W3009525672 @default.
- W4318755613 cites W3011827142 @default.
- W4318755613 cites W3014733932 @default.
- W4318755613 cites W3079783440 @default.
- W4318755613 cites W3081133783 @default.
- W4318755613 cites W3081658712 @default.
- W4318755613 cites W3081876028 @default.
- W4318755613 cites W3081918697 @default.
- W4318755613 cites W3083027972 @default.
- W4318755613 cites W3087100678 @default.
- W4318755613 cites W3099810371 @default.
- W4318755613 cites W3113922315 @default.
- W4318755613 cites W3131359651 @default.
- W4318755613 cites W3155727024 @default.
- W4318755613 cites W3161348944 @default.
- W4318755613 cites W3169802154 @default.
- W4318755613 cites W3183431649 @default.
- W4318755613 cites W3184717546 @default.
- W4318755613 cites W3202111261 @default.
- W4318755613 cites W3209211415 @default.
- W4318755613 cites W3215504321 @default.
- W4318755613 cites W4220751637 @default.
- W4318755613 cites W4220766107 @default.
- W4318755613 cites W4221027889 @default.
- W4318755613 cites W4248024654 @default.
- W4318755613 doi "https://doi.org/10.1021/jacs.2c12491" @default.
- W4318755613 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36724060" @default.
- W4318755613 hasPublicationYear "2023" @default.
- W4318755613 type Work @default.
- W4318755613 citedByCount "2" @default.
- W4318755613 countsByYear W43187556132023 @default.