Matches in SemOpenAlex for { <https://semopenalex.org/work/W4318774631> ?p ?o ?g. }
- W4318774631 endingPage "96" @default.
- W4318774631 startingPage "77" @default.
- W4318774631 abstract "BACKGROUND: Neuroblastoma is one of the most common extracranial solid tumors in childhood. At present, epigenetic disorders play a significant role in neoplasms development. Since epigenetic changes in the cell are quite dynamic and reversible, epigenome-modulating exogenous agents can be used in epigenetic targeted therapy for various types of tumors. Therefore, the identification of these agents is still significant. Lactoferrin is one such potential molecule from the transferrin family. Currently, the anti-tumor properties of lactoferrin have been identified, but its effect on the epigenome of cells of various tumors types, particularly on neuroblastomas, is practically unknown.
 AIM: To study the effect of the exogenous recombinant human apolactoferrin on the viability and epigenomic status of IMR-32 neuroblastoma cells.
 MATERIALS AND METHODS: We studied human IMR-32 neuroblastoma cells after 72 hours of exposure to 8 doses of recombinant human apolactoferrin: 0.1, 0.5, 1, 5, 10, 50, 100 and 500 g/ml. The level of genome-wide DNA methylation and the degree of chromatin compaction in IMR-32 cells were quantified using commercial kits 5-mC DNA ELISA Kit, Global DNA Methylation LINE-1 Kit, as well as enzymatic hydrolysis of MspI / HpaII and DNaseI.
 RESULTS: The recombinant apolactoferrin reduces the viability of IMR-32 and, depending on the dose, differentially affects the level of genome-wide DNA methylation (СpG dinucleotides, CCGG sites, LINE-1 repeats) and the degree of chromatin compaction. At the same time, a complex picture of the epigenomic cellular response to the effect of apo-lactoferrin was observed (nonlinear nonmonotonic dose-effect relationship).
 CONCLUSIONS: We assumed that apolactoferrin modulates gene activity through epigenetic mechanisms, in particular, by changing the DNA methylation pattern and affecting the chromatin structure, which may be one of the molecular mechanisms of its anti-tumor effect." @default.
- W4318774631 created "2023-02-02" @default.
- W4318774631 creator A5008311522 @default.
- W4318774631 creator A5050347523 @default.
- W4318774631 creator A5054546211 @default.
- W4318774631 creator A5063244962 @default.
- W4318774631 creator A5063450980 @default.
- W4318774631 creator A5078617846 @default.
- W4318774631 date "2023-02-01" @default.
- W4318774631 modified "2023-09-26" @default.
- W4318774631 title "Apo-form of recombinant human lactoferrin changes the genome-wide DNA methylation level and the chromatin compaction degree in neuroblastoma cell line IMR-32" @default.
- W4318774631 cites W1518480265 @default.
- W4318774631 cites W1542952446 @default.
- W4318774631 cites W1595170830 @default.
- W4318774631 cites W1825662645 @default.
- W4318774631 cites W1964381304 @default.
- W4318774631 cites W1967430000 @default.
- W4318774631 cites W1969601801 @default.
- W4318774631 cites W1970832400 @default.
- W4318774631 cites W1977920395 @default.
- W4318774631 cites W1986366861 @default.
- W4318774631 cites W1997775198 @default.
- W4318774631 cites W2006208579 @default.
- W4318774631 cites W2010234159 @default.
- W4318774631 cites W2011425303 @default.
- W4318774631 cites W2011951374 @default.
- W4318774631 cites W2012074131 @default.
- W4318774631 cites W2014852291 @default.
- W4318774631 cites W2015734897 @default.
- W4318774631 cites W2018310062 @default.
- W4318774631 cites W2030343821 @default.
- W4318774631 cites W2037624452 @default.
- W4318774631 cites W2050249780 @default.
- W4318774631 cites W2063444470 @default.
- W4318774631 cites W2069698188 @default.
- W4318774631 cites W2074565049 @default.
- W4318774631 cites W2074832122 @default.
- W4318774631 cites W2093684733 @default.
- W4318774631 cites W2099540110 @default.
- W4318774631 cites W2100212574 @default.
- W4318774631 cites W2106801689 @default.
- W4318774631 cites W2108684366 @default.
- W4318774631 cites W2115492334 @default.
- W4318774631 cites W2119453983 @default.
- W4318774631 cites W2123133207 @default.
- W4318774631 cites W2129738000 @default.
- W4318774631 cites W2144699098 @default.
- W4318774631 cites W2150127222 @default.
- W4318774631 cites W2152065348 @default.
- W4318774631 cites W2153896122 @default.
- W4318774631 cites W2156315349 @default.
- W4318774631 cites W2162292699 @default.
- W4318774631 cites W2222977590 @default.
- W4318774631 cites W2286231267 @default.
- W4318774631 cites W2343464241 @default.
- W4318774631 cites W2415859941 @default.
- W4318774631 cites W2463892076 @default.
- W4318774631 cites W2549852019 @default.
- W4318774631 cites W2601579284 @default.
- W4318774631 cites W2610326370 @default.
- W4318774631 cites W2736286144 @default.
- W4318774631 cites W2799474346 @default.
- W4318774631 cites W2801545162 @default.
- W4318774631 cites W2802619653 @default.
- W4318774631 cites W2904417088 @default.
- W4318774631 cites W2911958258 @default.
- W4318774631 cites W2913351746 @default.
- W4318774631 cites W2921408090 @default.
- W4318774631 cites W2932465313 @default.
- W4318774631 cites W2960183779 @default.
- W4318774631 cites W2967081996 @default.
- W4318774631 cites W2969699662 @default.
- W4318774631 cites W2971450830 @default.
- W4318774631 cites W2977262933 @default.
- W4318774631 cites W2981415489 @default.
- W4318774631 cites W3007613732 @default.
- W4318774631 cites W3010658987 @default.
- W4318774631 cites W3015918863 @default.
- W4318774631 cites W3046017795 @default.
- W4318774631 cites W3048536367 @default.
- W4318774631 cites W3094110744 @default.
- W4318774631 cites W3121017260 @default.
- W4318774631 cites W3136017014 @default.
- W4318774631 cites W3166767779 @default.
- W4318774631 cites W3183953983 @default.
- W4318774631 cites W4220823311 @default.
- W4318774631 cites W3048898054 @default.
- W4318774631 doi "https://doi.org/10.17816/maj112498" @default.
- W4318774631 hasPublicationYear "2023" @default.
- W4318774631 type Work @default.
- W4318774631 citedByCount "0" @default.
- W4318774631 crossrefType "journal-article" @default.
- W4318774631 hasAuthorship W4318774631A5008311522 @default.
- W4318774631 hasAuthorship W4318774631A5050347523 @default.
- W4318774631 hasAuthorship W4318774631A5054546211 @default.
- W4318774631 hasAuthorship W4318774631A5063244962 @default.
- W4318774631 hasAuthorship W4318774631A5063450980 @default.
- W4318774631 hasAuthorship W4318774631A5078617846 @default.