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- W4319061796 abstract "Article3 February 2023 Source DataTransparent process Extracellular vesicles from Zika virus-infected cells display viral E protein that binds ZIKV-neutralizing antibodies to prevent infection enhancement Fanfan Zhao Fanfan Zhao Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Biosafety Level 3 Laboratory, Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, China CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Validation, Investigation, Methodology Search for more papers by this author Yongfen Xu Yongfen Xu orcid.org/0000-0002-2845-7753 CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Resources, Validation, Investigation Search for more papers by this author Na Liu Na Liu orcid.org/0000-0002-5313-9888 Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Biosafety Level 3 Laboratory, Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, China Contribution: Validation, Investigation, Methodology, Writing - review & editing Search for more papers by this author Dawei Lv Dawei Lv orcid.org/0000-0001-8567-2137 CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Investigation Search for more papers by this author Yujie Chen Yujie Chen orcid.org/0000-0003-0666-5874 CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Investigation Search for more papers by this author Zhi Liu Zhi Liu CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Search for more papers by this author Xia Jin Xia Jin CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Resources, Methodology Search for more papers by this author Mingbing Xiao Mingbing Xiao orcid.org/0000-0003-3372-1069 Department of Gastroenterology and Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, China Contribution: Resources, Validation, Investigation Search for more papers by this author Dimitri Lavillette Dimitri Lavillette orcid.org/0000-0002-4706-1519 CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Formal analysis, Methodology Search for more papers by this author Jin Zhong Jin Zhong CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Resources Search for more papers by this author Ralf Bartenschlager Ralf Bartenschlager Department of Infectious Diseases, Molecular Virology, Heidelberg University, Heidelberg, Germany German Center for Infectious Diseases, Heidelberg Partner Site, Heidelberg, Germany Contribution: Data curation, Formal analysis Search for more papers by this author Gang Long Corresponding Author Gang Long [email protected] orcid.org/0000-0002-5078-4902 Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Biosafety Level 3 Laboratory, Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, China CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Conceptualization, Data curation, Funding acquisition, Investigation, Methodology, Writing - review & editing Search for more papers by this author Fanfan Zhao Fanfan Zhao Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Biosafety Level 3 Laboratory, Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, China CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Validation, Investigation, Methodology Search for more papers by this author Yongfen Xu Yongfen Xu orcid.org/0000-0002-2845-7753 CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Resources, Validation, Investigation Search for more papers by this author Na Liu Na Liu orcid.org/0000-0002-5313-9888 Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Biosafety Level 3 Laboratory, Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, China Contribution: Validation, Investigation, Methodology, Writing - review & editing Search for more papers by this author Dawei Lv Dawei Lv orcid.org/0000-0001-8567-2137 CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Investigation Search for more papers by this author Yujie Chen Yujie Chen orcid.org/0000-0003-0666-5874 CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Investigation Search for more papers by this author Zhi Liu Zhi Liu CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Search for more papers by this author Xia Jin Xia Jin CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Resources, Methodology Search for more papers by this author Mingbing Xiao Mingbing Xiao orcid.org/0000-0003-3372-1069 Department of Gastroenterology and Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, China Contribution: Resources, Validation, Investigation Search for more papers by this author Dimitri Lavillette Dimitri Lavillette orcid.org/0000-0002-4706-1519 CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Formal analysis, Methodology Search for more papers by this author Jin Zhong Jin Zhong CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Resources Search for more papers by this author Ralf Bartenschlager Ralf Bartenschlager Department of Infectious Diseases, Molecular Virology, Heidelberg University, Heidelberg, Germany German Center for Infectious Diseases, Heidelberg Partner Site, Heidelberg, Germany Contribution: Data curation, Formal analysis Search for more papers by this author Gang Long Corresponding Author Gang Long [email protected] orcid.org/0000-0002-5078-4902 Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Biosafety Level 3 Laboratory, Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, China CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Contribution: Conceptualization, Data curation, Funding acquisition, Investigation, Methodology, Writing - review & editing Search for more papers by this author Author Information Fanfan Zhao1,2,†, Yongfen Xu2,†, Na Liu1,†, Dawei Lv2, Yujie Chen2, Zhi Liu2, Xia Jin2, Mingbing Xiao3, Dimitri Lavillette2, Jin Zhong2, Ralf Bartenschlager4,5 and Gang Long *,1,2 1Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Biosafety Level 3 Laboratory, Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, China 2CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China 3Department of Gastroenterology and Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, China 4Department of Infectious Diseases, Molecular Virology, Heidelberg University, Heidelberg, Germany 5German Center for Infectious Diseases, Heidelberg Partner Site, Heidelberg, Germany † These authors contributed equally to this work *Corresponding author. Tel: 0086-021-54237017; E-mail: [email protected] The EMBO Journal (2023)42:e112096https://doi.org/10.15252/embj.2022112096 Full textView the full text of the articlePDFDownload PDF of article text and main figures. Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Abstract Mosquito-borne flaviviruses including Zika virus (ZIKV) represent a public health problem in some parts of the world. Although ZIKV infection is predominantly asymptomatic or associated with mild symptoms, it can lead to neurological complications. ZIKV infection can also cause antibody-dependent enhancement (ADE) of infection with similar viruses, warranting further studies of virion assembly and the function of envelope (E) protein-specific antibodies. Although extracellular vesicles (EVs) from flavivirus-infected cells have been reported to transmit infection, this interpretation is challenged by difficulties in separating EVs from flavivirions due to their similar biochemical composition and biophysical properties. In the present study, a rigorous EV-virion separation method combining sequential ultracentrifugation and affinity capture was developed to study EVs from ZIKV-infected cells. We find that these EVs do not transmit infection, but EVs display abundant E proteins which have an antigenic landscape similar to that of virions carrying E. ZIKV E-coated EVs attenuate antibody-dependent enhancement mediated by ZIKV E-specific and DENV-cross-reactive antibodies in both cell culture and mouse models. We thus report an alternative route for Flavivirus E protein secretion. These results suggest that modulation of E protein release via virions and EVs may present a new approach to regulating flavivirus-host interactions. Synopsis Flavivirus-infected cells have been reported to secrete extracellular vesicles (EVs) that are able to transmit the infection. An optimized purification protocol shows that EVs derived from cells infected with Zika or dengue viruses do not mediate infection but display viral envelope (E) proteins. CD9-affinity captured EVs from ZIKV-infected cells contain viral RNA, but are not infectious in cell culture and in a mouse model. EVs derived from ZIKV- or DENV-infected cells display viral E protein on the surface. Flavivirus E protein-coated EVs attenuate antibody-dependent enhancement mediated by both monoclonal antibodies and patient sera at subneutralizing concentration. Previous ArticleNext Article Read MoreAbout the coverClose modalView large imageVolume 42,Issue 6,15 March 2023This cover highlights the article The cryo‐EM structure of the CENP‐A nucleosome in complex with ggKNL2 by Honghui Jiang, Mariko Ariyoshi, Tatsuo Fukugawa and colleagues. The CENP‐A nucleosome is the fundamental architecture of the centromere responsible for chromosome segregation. KNL2 is one of key factors for new CENP‐A deposition into the centromeres. Here, using cryo‐EM, biochemical and cell biology experiments, we show that chicken KNL2, instead of CENP‐C, binds to the CENP‐A (green) nucleosome via the conserved CENP‐C like motif (orange) in interphase to maintain the CENP‐A nucleosome in centromeres. In mitosis, phosphorylated CENP‐C dominantly binds to the CENP‐A nucleosome. Our findings provide a new insight into dynamic regulation of structural change of the centromere during the cell cycle progression.(Scientific image by the authors) Volume 42Issue 615 March 2023In this issue RelatedDetailsLoading ..." @default.
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- W4319061796 title "Extracellular vesicles from Zika virus‐infected cells display viral E protein that binds ZIKV‐neutralizing antibodies to prevent infection enhancement" @default.
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