Matches in SemOpenAlex for { <https://semopenalex.org/work/W4319312169> ?p ?o ?g. }
- W4319312169 abstract "The detection of proteins is of central importance to biomolecular analysis and diagnostics. Typical immunosensing assays rely on surface-capture of target molecules, but this constraint can limit specificity, sensitivity, and the ability to obtain information beyond simple concentration measurements. Here we present a surface-free, single-molecule microfluidic sensing platform for direct digital protein biomarker detection in solution, termed digital immunosensor assay (DigitISA). DigitISA is based on microchip electrophoretic separation combined with single-molecule detection and enables absolute number/concentration quantification of proteins in a single, solution-phase step. Applying DigitISA to a range of targets including amyloid aggregates, exosomes, and biomolecular condensates, we demonstrate that the assay provides information beyond stoichiometric interactions, and enables characterization of immunochemistry, binding affinity, and protein biomarker abundance. Taken together, our results suggest a experimental paradigm for the sensing of protein biomarkers, which enables analyses of targets that are challenging to address using conventional immunosensing approaches." @default.
- W4319312169 created "2023-02-08" @default.
- W4319312169 creator A5001045596 @default.
- W4319312169 creator A5002289292 @default.
- W4319312169 creator A5008277442 @default.
- W4319312169 creator A5019000351 @default.
- W4319312169 creator A5024777293 @default.
- W4319312169 creator A5031836910 @default.
- W4319312169 creator A5041227038 @default.
- W4319312169 creator A5041530719 @default.
- W4319312169 creator A5042822683 @default.
- W4319312169 creator A5048226666 @default.
- W4319312169 creator A5057736494 @default.
- W4319312169 creator A5057988701 @default.
- W4319312169 creator A5060855672 @default.
- W4319312169 creator A5064979540 @default.
- W4319312169 creator A5076048603 @default.
- W4319312169 creator A5076879004 @default.
- W4319312169 creator A5084999897 @default.
- W4319312169 creator A5087589231 @default.
- W4319312169 date "2023-02-06" @default.
- W4319312169 modified "2023-10-05" @default.
- W4319312169 title "Direct digital sensing of protein biomarkers in solution" @default.
- W4319312169 cites W1555009525 @default.
- W4319312169 cites W1762620449 @default.
- W4319312169 cites W1831899038 @default.
- W4319312169 cites W1906064333 @default.
- W4319312169 cites W1952608303 @default.
- W4319312169 cites W1965500282 @default.
- W4319312169 cites W1966130903 @default.
- W4319312169 cites W1969077673 @default.
- W4319312169 cites W1969196565 @default.
- W4319312169 cites W1970611232 @default.
- W4319312169 cites W1987125442 @default.
- W4319312169 cites W1995830102 @default.
- W4319312169 cites W1997305257 @default.
- W4319312169 cites W2006506298 @default.
- W4319312169 cites W2006813546 @default.
- W4319312169 cites W2017560560 @default.
- W4319312169 cites W2026908430 @default.
- W4319312169 cites W2037546764 @default.
- W4319312169 cites W2050375429 @default.
- W4319312169 cites W2060924363 @default.
- W4319312169 cites W2076924877 @default.
- W4319312169 cites W2079321730 @default.
- W4319312169 cites W2080681593 @default.
- W4319312169 cites W2082607189 @default.
- W4319312169 cites W2083324547 @default.
- W4319312169 cites W2084373725 @default.
- W4319312169 cites W2084855813 @default.
- W4319312169 cites W2092198587 @default.
- W4319312169 cites W2093451229 @default.
- W4319312169 cites W2102575998 @default.
- W4319312169 cites W2104525153 @default.
- W4319312169 cites W2106044600 @default.
- W4319312169 cites W2146103350 @default.
- W4319312169 cites W2152345838 @default.
- W4319312169 cites W2160312052 @default.
- W4319312169 cites W2161863487 @default.
- W4319312169 cites W2163074782 @default.
- W4319312169 cites W2164039871 @default.
- W4319312169 cites W2168958678 @default.
- W4319312169 cites W2321181342 @default.
- W4319312169 cites W2330515185 @default.
- W4319312169 cites W2336750746 @default.
- W4319312169 cites W2470936315 @default.
- W4319312169 cites W2508534590 @default.
- W4319312169 cites W2588636729 @default.
- W4319312169 cites W2590568317 @default.
- W4319312169 cites W2603284132 @default.
- W4319312169 cites W2619239407 @default.
- W4319312169 cites W2756821926 @default.
- W4319312169 cites W2763352793 @default.
- W4319312169 cites W2773410186 @default.
- W4319312169 cites W2781579322 @default.
- W4319312169 cites W2793668205 @default.
- W4319312169 cites W2793939127 @default.
- W4319312169 cites W2797600975 @default.
- W4319312169 cites W2802669613 @default.
- W4319312169 cites W2810327952 @default.
- W4319312169 cites W2886119468 @default.
- W4319312169 cites W2891024355 @default.
- W4319312169 cites W2894386983 @default.
- W4319312169 cites W2909998782 @default.
- W4319312169 cites W2921355001 @default.
- W4319312169 cites W2940371405 @default.
- W4319312169 cites W2944565102 @default.
- W4319312169 cites W2950088946 @default.
- W4319312169 cites W2953175648 @default.
- W4319312169 cites W2954966126 @default.
- W4319312169 cites W2995650811 @default.
- W4319312169 cites W2998881619 @default.
- W4319312169 cites W3005104290 @default.
- W4319312169 cites W3015116555 @default.
- W4319312169 cites W3015856745 @default.
- W4319312169 cites W3016040333 @default.
- W4319312169 cites W3016762840 @default.
- W4319312169 cites W3037902661 @default.
- W4319312169 cites W3048449967 @default.
- W4319312169 cites W3081213296 @default.