FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4319337051> ?p ?o ?g. }

• W4319337051 abstract "Abstract Background : Pancreatic cancer is a highly progressive cancer with limited effective treatment. The tumor microenvironment in pancreatic cancer is aggressive and severely hypoxic with a high immunosuppressive effect. This has raised the attention to understanding the pancreatic cancer microenvironment and the interaction between pancreatic cancer cells and cytotoxic T-cells. Objective : This study aimed to investigate the crosstalk between hypoxic pancreatic cancer cells (PANC-1) and cytotoxic CD8+ T-cells. Methods : Pancreatic cancer cells (PANC-1) were exposed to 20 cycles of chronic hypoxic conditions for 72 hours followed by a re-oxygenation period for 24 hours. On cycles 10 and 20, PANC1 hypoxic conditioned media (CM) were harvested and the hypoxic PANC-1 cells were co-cultured with the activated cytotoxic CD8+ T-cells or with CD8+ T-cells CM. CD8+ T-cells CM was collected after 5 days of cell activation using anti-CD3/CD28 antibodies and interleukin-2 (IL-2). On the other hand, CD8+ T-cells were activated for 72 hours, and then cultured with the hypoxic PANC-1 CM. Results : Hypoxic PANC-1 cells showed a significant increase in resistance to the lytic effect of either CD8+ T-cells coculture or CD8+ T-cells CM treatment when compared with normoxic PANC-1 cells. A significant decrease in TNF-α and IFN-γ levels was also detected. Additionally, a significant increase in IL-6, P53 and TNF-α gene expression levels was observed in PANC-1 cells treated with CD8+ T-cells CM. Moreover, IL-6 gene expression level showed a significant difference between hypoxic and normoxic PANC-1 cells. Furthermore, CD8+ T-cells proliferation and cytokines production were significantly higher in cells cultured with PANC-1 CM. However, no significant differences were observed after treatment with either hypoxic or normoxic PANC-1 CM. Conclusion : Hypoxia influences PANC-1 cells' sensitivity to cytotoxic CD8+ T-cells. Several mechanisms of PANC-1 resistance were detected in this study; an increase in IL-6 production and a depletion in TNF-α and IFN-γ levels when co-cultured with PANC-1 cells. In treated PANC-1 cells, an increase in TP53 and TNF-α gene expression were observed. IL-6 activation is one of the mechanisms that may increase the resistance to CD8+ T-cells in hypoxic PANC-1 cells." @default.
• W4319337051 created "2023-02-08" @default.
• W4319337051 creator A5003547004 @default.
• W4319337051 creator A5019989611 @default.
• W4319337051 creator A5023610432 @default.
• W4319337051 creator A5025791687 @default.
• W4319337051 creator A5052834032 @default.
• W4319337051 creator A5066256419 @default.
• W4319337051 date "2023-02-07" @default.
• W4319337051 modified "2023-10-18" @default.
• W4319337051 title "The Effect of Cytotoxic CD8+ T-Cells Secretory Proteins on Hypoxic Pancreatic Cancer Cells" @default.
• W4319337051 cites W1447783661 @default.
• W4319337051 cites W1799143857 @default.
• W4319337051 cites W1972648258 @default.
• W4319337051 cites W1987520066 @default.
• W4319337051 cites W1997843836 @default.
• W4319337051 cites W2050558251 @default.
• W4319337051 cites W2054064720 @default.
• W4319337051 cites W2072954179 @default.
• W4319337051 cites W2100412095 @default.
• W4319337051 cites W2102073127 @default.
• W4319337051 cites W2136348963 @default.
• W4319337051 cites W2140163728 @default.
• W4319337051 cites W2145796213 @default.
• W4319337051 cites W2165060728 @default.
• W4319337051 cites W2166053801 @default.
• W4319337051 cites W2170903814 @default.
• W4319337051 cites W2177971463 @default.
• W4319337051 cites W2204695292 @default.
• W4319337051 cites W2294951120 @default.
• W4319337051 cites W2313942882 @default.
• W4319337051 cites W2409057459 @default.
• W4319337051 cites W2511927825 @default.
• W4319337051 cites W2527008427 @default.
• W4319337051 cites W2537220355 @default.
• W4319337051 cites W2552550451 @default.
• W4319337051 cites W2559117153 @default.
• W4319337051 cites W2613663785 @default.
• W4319337051 cites W2620653914 @default.
• W4319337051 cites W2739635003 @default.
• W4319337051 cites W2770650133 @default.
• W4319337051 cites W2773651127 @default.
• W4319337051 cites W2789637558 @default.
• W4319337051 cites W2793547685 @default.
• W4319337051 cites W2802731336 @default.
• W4319337051 cites W2805717146 @default.
• W4319337051 cites W2884691005 @default.
• W4319337051 cites W2886428274 @default.
• W4319337051 cites W2889474158 @default.
• W4319337051 cites W2895357007 @default.
• W4319337051 cites W2898368508 @default.
• W4319337051 cites W2901134185 @default.
• W4319337051 cites W2901513648 @default.
• W4319337051 cites W2911058506 @default.
• W4319337051 cites W2911823544 @default.
• W4319337051 cites W2913836031 @default.
• W4319337051 cites W2942594871 @default.
• W4319337051 cites W2942960289 @default.
• W4319337051 cites W2964416380 @default.
• W4319337051 cites W2967470918 @default.
• W4319337051 cites W2969843242 @default.
• W4319337051 cites W2970486139 @default.
• W4319337051 cites W2989356502 @default.
• W4319337051 cites W3005568783 @default.
• W4319337051 cites W3009145316 @default.
• W4319337051 cites W3016364844 @default.
• W4319337051 cites W3048585942 @default.
• W4319337051 cites W3052273069 @default.
• W4319337051 cites W3080758074 @default.
• W4319337051 cites W3112344668 @default.
• W4319337051 cites W3126837324 @default.
• W4319337051 cites W4250196117 @default.
• W4319337051 doi "https://doi.org/10.21203/rs.3.rs-2505088/v1" @default.
• W4319337051 hasPublicationYear "2023" @default.
• W4319337051 type Work @default.
• W4319337051 citedByCount "0" @default.
• W4319337051 crossrefType "posted-content" @default.
• W4319337051 hasAuthorship W4319337051A5003547004 @default.
• W4319337051 hasAuthorship W4319337051A5019989611 @default.
• W4319337051 hasAuthorship W4319337051A5023610432 @default.
• W4319337051 hasAuthorship W4319337051A5025791687 @default.
• W4319337051 hasAuthorship W4319337051A5052834032 @default.
• W4319337051 hasAuthorship W4319337051A5066256419 @default.
• W4319337051 hasBestOaLocation W43193370511 @default.
• W4319337051 hasConcept C121608353 @default.
• W4319337051 hasConcept C126322002 @default.
• W4319337051 hasConcept C148125776 @default.
• W4319337051 hasConcept C149532602 @default.
• W4319337051 hasConcept C154317977 @default.
• W4319337051 hasConcept C167672396 @default.
• W4319337051 hasConcept C185592680 @default.
• W4319337051 hasConcept C202751555 @default.
• W4319337051 hasConcept C203014093 @default.
• W4319337051 hasConcept C2776107976 @default.
• W4319337051 hasConcept C2780210213 @default.
• W4319337051 hasConcept C502942594 @default.
• W4319337051 hasConcept C55493867 @default.
• W4319337051 hasConcept C71924100 @default.
• W4319337051 hasConcept C86803240 @default.
• W4319337051 hasConcept C8891405 @default.

• next