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- W4319337592 abstract "A total of ten lactobacillus strains were isolated from broiler chickens and their probiotic properties including tolerance to gastrointestinal fluids and heat treatment, antimicrobial activity, adhesion capacity to intestinal cells, surface hydrophobicity, autoaggregation, antioxidative activity and immunomodulatory effects on chicken macrophages were evaluated. The Limosilactobacillus reuteri (LR) was the most frequently isolated species, followed by Lactobacillus johnsonii (LJ) and Ligilactobacillus salivarius (LS). All isolates showed good resistance to simulated gastrointestinal conditions and antimicrobial activity against four indicator strains including Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae and Proteus mirabilis. LR 21 exhibited excellent performances on autoaggregation, hydrophobicity and adhesion capacity to Caco-2 intestinal cells. In the meantime, this strain also possessed considerable tolerance to heat treatment, which indicated great potential to be used in the feed industry. However, LJ 20 strain had the highest free radical scavenging activity compared with the other strains. Furthermore, qRT-PCR results revealed that all isolated strains significantly increased the transcriptional levels of pro-inflammatory genes and tended to induce the M1-type polarization on HD11 macrophages. Particularly, the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) was adopted in our study to compare and select the most promising probiotic candidate based on in vitro evaluation tests." @default.
- W4319337592 created "2023-02-08" @default.
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- W4319337592 date "2023-05-01" @default.
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- W4319337592 title "Probiotic characterization and comparison of broiler-derived lactobacillus strains based on technique for order preference by similarity to ideal solution analysis" @default.
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- W4319337592 doi "https://doi.org/10.1016/j.psj.2023.102564" @default.
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