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- W4319442615 abstract "Abstract Amide syntheses remain a key challenging green chemistry reaction. For instance, green synthesis of N ‐acyl glycines as biosurfactants and therapeutics is highly desirable to replace chemical pathways using toxic phosgene. Herein, we report a novel concept for enzymatic amidation in an aqueous system via glycerol activation of fatty acids and theirsubsequent aminolysis with glycine to synthesize N ‐acyl glycines. We then engineer an enzyme (proRML) by reshaping its catalytic pocket to enhance its aminolysis activity and catalytic efficiency by 103‐fold and 465‐fold, respectively. The evolved proRML (D156S/L258K/L267N/S83D/L58K/R86K/W88V) catalyzed the amidation of a fatty acid with glycine to give N ‐lauroylglycine with high yield (80 %). It accepts a broad range of medium‐ to long‐chain fatty acids (C 8 –C 18 ), giving high yields of N ‐decanoyl‐, N ‐myristoyl‐, and N ‐oleoylglycine. The developed amidation concept may be general, and the engineered enzyme is useful for the green synthesis of N ‐acyl glycines." @default.
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- W4319442615 date "2023-02-21" @default.
- W4319442615 modified "2023-09-27" @default.
- W4319442615 title "Enzyme Engineering for High‐Yielding Amide Formation: Lipase‐Catalyzed Synthesis of <i>N</i>‐Acyl Glycines in Aqueous Media" @default.
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- W4319442615 doi "https://doi.org/10.1002/ange.202217878" @default.
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