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- W4319460003 abstract "Implantable cardiovascular therapeutic devices (CTD) including stents, percutaneous heart valves and ventricular assist devices, while lifesaving, impart supraphysiologic shear stress to platelets resulting in thrombotic and bleeding device-related coagulopathy. We previously demonstrated that shear-mediated platelet dysfunction is associated with downregulation of platelet GPIb-IX-V and αIIbβ3 receptors via generation of platelet-derived microparticles (PDMPs). Here, we test the hypothesis that shear-generated PDMPs manifest phenotypical heterogeneity of their morphology and surface expression of platelet receptors, and modulate platelet hemostatic function.Human gel-filtered platelets were exposed to continuous shear stress and sonication. Alterations of platelet morphology were visualized using transmission electron microscopy. Surface expression of platelet receptors and PDMP generation were quantified by flow cytometry. Thrombin generation was quantified spectrophotometrically, and platelet aggregation in plasma was measured by optical aggregometry. We demonstrate that platelet exposure to shear stress promotes notable alterations in platelet morphology and ejection of several distinctive types of PDMPs. Shear-mediated microvesiculation is associated with the differential remodeling of platelet receptors with PDMPs expressing significantly higher levels of both adhesion (α IIb β 3 , GPIX, PECAM-1, P-selectin, and PSGL-1) and agonist-evoked receptors (P 2 Y 12 & PAR1). Shear-mediated PDMPs have a bidirectional effect on platelet hemostatic function, promoting thrombin generation and inhibiting platelet aggregation induced by collagen and ADP.Shear-generated PDMPs demonstrate phenotypic heterogeneity as to morphologic features and defined patterns of surface receptor alteration, and impose a bidirectional effect on platelet hemostatic function. PDMP heterogeneity suggests that a range of mechanisms are operative in the microvesiculation process, contributing to CTD coagulopathy and posing opportunities for therapeutic manipulation." @default.
- W4319460003 created "2023-02-09" @default.
- W4319460003 creator A5002274002 @default.
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- W4319460003 date "2023-02-08" @default.
- W4319460003 modified "2023-09-28" @default.
- W4319460003 title "Shear-Mediated Platelet Microparticles Demonstrate Phenotypic Heterogeneity as to Morphology, Receptor Distribution, and Hemostatic Function" @default.
- W4319460003 cites W1487701486 @default.
- W4319460003 cites W1516806117 @default.
- W4319460003 cites W1546773255 @default.
- W4319460003 cites W1551523049 @default.
- W4319460003 cites W1757310287 @default.
- W4319460003 cites W1826819713 @default.
- W4319460003 cites W1833080940 @default.
- W4319460003 cites W1850586 @default.
- W4319460003 cites W1909159002 @default.
- W4319460003 cites W1963549684 @default.
- W4319460003 cites W1970522778 @default.
- W4319460003 cites W1973319320 @default.
- W4319460003 cites W1979069643 @default.
- W4319460003 cites W1982177481 @default.
- W4319460003 cites W1984316467 @default.
- W4319460003 cites W1984909109 @default.
- W4319460003 cites W1991525001 @default.
- W4319460003 cites W1994494585 @default.
- W4319460003 cites W1997787147 @default.
- W4319460003 cites W1997860246 @default.
- W4319460003 cites W1998100237 @default.
- W4319460003 cites W2005526270 @default.
- W4319460003 cites W2010546098 @default.
- W4319460003 cites W2015445251 @default.
- W4319460003 cites W2015877328 @default.
- W4319460003 cites W2015948321 @default.
- W4319460003 cites W2016531738 @default.
- W4319460003 cites W2029004784 @default.
- W4319460003 cites W2032356038 @default.
- W4319460003 cites W2049816773 @default.
- W4319460003 cites W2054265385 @default.
- W4319460003 cites W2055583156 @default.
- W4319460003 cites W2058629481 @default.
- W4319460003 cites W2064070904 @default.
- W4319460003 cites W206565180 @default.
- W4319460003 cites W2070320911 @default.
- W4319460003 cites W2071434431 @default.
- W4319460003 cites W2072268350 @default.
- W4319460003 cites W2073121233 @default.
- W4319460003 cites W2074668374 @default.
- W4319460003 cites W2080010451 @default.
- W4319460003 cites W2080723162 @default.
- W4319460003 cites W2092850858 @default.
- W4319460003 cites W2112504623 @default.
- W4319460003 cites W2116612435 @default.
- W4319460003 cites W2117156869 @default.
- W4319460003 cites W2119286443 @default.
- W4319460003 cites W2122187912 @default.
- W4319460003 cites W2123520441 @default.
- W4319460003 cites W2123661270 @default.
- W4319460003 cites W2127543143 @default.
- W4319460003 cites W2128139337 @default.
- W4319460003 cites W2128214001 @default.
- W4319460003 cites W2129447163 @default.
- W4319460003 cites W2129888900 @default.
- W4319460003 cites W2130007894 @default.
- W4319460003 cites W2132717546 @default.
- W4319460003 cites W2133780665 @default.
- W4319460003 cites W2139967807 @default.
- W4319460003 cites W2141445903 @default.
- W4319460003 cites W2141905650 @default.
- W4319460003 cites W2141906699 @default.
- W4319460003 cites W2157716190 @default.
- W4319460003 cites W2160616952 @default.
- W4319460003 cites W2164027309 @default.
- W4319460003 cites W2167141073 @default.
- W4319460003 cites W2177998542 @default.
- W4319460003 cites W2179514190 @default.
- W4319460003 cites W2295508527 @default.
- W4319460003 cites W2329252178 @default.
- W4319460003 cites W2331820709 @default.
- W4319460003 cites W2419958883 @default.
- W4319460003 cites W2481038056 @default.
- W4319460003 cites W2500142620 @default.
- W4319460003 cites W2538622663 @default.
- W4319460003 cites W2552399529 @default.
- W4319460003 cites W2553350361 @default.
- W4319460003 cites W2672945187 @default.
- W4319460003 cites W2750064151 @default.
- W4319460003 cites W2759155555 @default.
- W4319460003 cites W2784146546 @default.
- W4319460003 cites W2800058342 @default.
- W4319460003 cites W2803578821 @default.
- W4319460003 cites W2805188498 @default.
- W4319460003 cites W2891851394 @default.
- W4319460003 cites W2902903841 @default.
- W4319460003 cites W2910566786 @default.