Matches in SemOpenAlex for { <https://semopenalex.org/work/W4319462046> ?p ?o ?g. }
- W4319462046 endingPage "166" @default.
- W4319462046 startingPage "158" @default.
- W4319462046 abstract "Hypoxia has been identified as a major factor in the pathogenesis of adipose tissue inflammation, which is a hallmark of obesity and obesity-linked type 2 diabetes mellitus. In this study, we have investigated the impact of hypoxia (1% oxygen) on the physiology and metabolism of 3T3-L1 adipocytes, a widely used cell culture model of adipose. Specifically, we applied parallel labeling experiments, isotopomer spectral analysis, and 13C-metabolic flux analysis to quantify the impact of hypoxia on adipogenesis, de novo lipogenesis and metabolic flux reprogramming in adipocytes. We found that 3T3-L1 cells can successfully differentiate into lipid-accumulating adipocytes under hypoxia, although the production of lipids was reduced by about 40%. Quantitative flux analysis demonstrated that short-term (1 day) and long-term (7 days) exposure to hypoxia resulted in similar reprogramming of cellular metabolism. Overall, we found that hypoxia: 1) reduced redox and energy generation by more than 2-fold and altered the patterns of metabolic pathway contributions to production and consumption of energy and redox cofactors; 2) redirected glucose metabolism from pentose phosphate pathway and citric acid cycle to lactate production; 3) rewired glutamine metabolism, from net glutamine production to net glutamine catabolism; 4) suppressed branched chain amino acid consumption; and 5) reduced biosynthesis of odd-chain fatty acids and mono-unsaturated fatty acids, while synthesis of saturated even-chain fatty acids was not affected. Together, these results highlight the profound impact of extracellular microenvironment on adipocyte metabolic activity and function." @default.
- W4319462046 created "2023-02-09" @default.
- W4319462046 creator A5055402397 @default.
- W4319462046 creator A5060360737 @default.
- W4319462046 date "2023-03-01" @default.
- W4319462046 modified "2023-10-14" @default.
- W4319462046 title "13C-Metabolic flux analysis of 3T3-L1 adipocytes illuminates its core metabolism under hypoxia" @default.
- W4319462046 cites W1781745633 @default.
- W4319462046 cites W1976552586 @default.
- W4319462046 cites W1985497859 @default.
- W4319462046 cites W1998464620 @default.
- W4319462046 cites W2000548772 @default.
- W4319462046 cites W2002041700 @default.
- W4319462046 cites W2014323212 @default.
- W4319462046 cites W2023832883 @default.
- W4319462046 cites W2028449239 @default.
- W4319462046 cites W2033471108 @default.
- W4319462046 cites W2052542261 @default.
- W4319462046 cites W2064119981 @default.
- W4319462046 cites W2065785909 @default.
- W4319462046 cites W2072022153 @default.
- W4319462046 cites W2079195246 @default.
- W4319462046 cites W2089928840 @default.
- W4319462046 cites W2091990362 @default.
- W4319462046 cites W2092493462 @default.
- W4319462046 cites W2100503932 @default.
- W4319462046 cites W2102761049 @default.
- W4319462046 cites W2108262958 @default.
- W4319462046 cites W2112850039 @default.
- W4319462046 cites W2114412752 @default.
- W4319462046 cites W2117731142 @default.
- W4319462046 cites W2130764919 @default.
- W4319462046 cites W2150350398 @default.
- W4319462046 cites W2159748100 @default.
- W4319462046 cites W2179192278 @default.
- W4319462046 cites W2219205942 @default.
- W4319462046 cites W2302911970 @default.
- W4319462046 cites W2336833178 @default.
- W4319462046 cites W2345428922 @default.
- W4319462046 cites W2405624971 @default.
- W4319462046 cites W2462095501 @default.
- W4319462046 cites W2504965130 @default.
- W4319462046 cites W2796927648 @default.
- W4319462046 cites W2895794785 @default.
- W4319462046 cites W2970801850 @default.
- W4319462046 cites W3096266289 @default.
- W4319462046 cites W3117237609 @default.
- W4319462046 cites W3156524618 @default.
- W4319462046 cites W3215448967 @default.
- W4319462046 cites W4200367364 @default.
- W4319462046 cites W974561090 @default.
- W4319462046 doi "https://doi.org/10.1016/j.ymben.2023.02.002" @default.
- W4319462046 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36758664" @default.
- W4319462046 hasPublicationYear "2023" @default.
- W4319462046 type Work @default.
- W4319462046 citedByCount "2" @default.
- W4319462046 countsByYear W43194620462023 @default.
- W4319462046 crossrefType "journal-article" @default.
- W4319462046 hasAuthorship W4319462046A5055402397 @default.
- W4319462046 hasAuthorship W4319462046A5060360737 @default.
- W4319462046 hasConcept C122927707 @default.
- W4319462046 hasConcept C141359234 @default.
- W4319462046 hasConcept C160941953 @default.
- W4319462046 hasConcept C165135838 @default.
- W4319462046 hasConcept C171089720 @default.
- W4319462046 hasConcept C178790620 @default.
- W4319462046 hasConcept C185592680 @default.
- W4319462046 hasConcept C192989942 @default.
- W4319462046 hasConcept C20251656 @default.
- W4319462046 hasConcept C2776175234 @default.
- W4319462046 hasConcept C2776991927 @default.
- W4319462046 hasConcept C2779349466 @default.
- W4319462046 hasConcept C3409486 @default.
- W4319462046 hasConcept C515207424 @default.
- W4319462046 hasConcept C55493867 @default.
- W4319462046 hasConcept C62231903 @default.
- W4319462046 hasConcept C68709404 @default.
- W4319462046 hasConcept C82714985 @default.
- W4319462046 hasConcept C86803240 @default.
- W4319462046 hasConcept C96942376 @default.
- W4319462046 hasConceptScore W4319462046C122927707 @default.
- W4319462046 hasConceptScore W4319462046C141359234 @default.
- W4319462046 hasConceptScore W4319462046C160941953 @default.
- W4319462046 hasConceptScore W4319462046C165135838 @default.
- W4319462046 hasConceptScore W4319462046C171089720 @default.
- W4319462046 hasConceptScore W4319462046C178790620 @default.
- W4319462046 hasConceptScore W4319462046C185592680 @default.
- W4319462046 hasConceptScore W4319462046C192989942 @default.
- W4319462046 hasConceptScore W4319462046C20251656 @default.
- W4319462046 hasConceptScore W4319462046C2776175234 @default.
- W4319462046 hasConceptScore W4319462046C2776991927 @default.
- W4319462046 hasConceptScore W4319462046C2779349466 @default.
- W4319462046 hasConceptScore W4319462046C3409486 @default.
- W4319462046 hasConceptScore W4319462046C515207424 @default.
- W4319462046 hasConceptScore W4319462046C55493867 @default.
- W4319462046 hasConceptScore W4319462046C62231903 @default.
- W4319462046 hasConceptScore W4319462046C68709404 @default.
- W4319462046 hasConceptScore W4319462046C82714985 @default.