FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4319662956> ?p ?o ?g. }

• W4319662956 endingPage "109675" @default.
• W4319662956 startingPage "109675" @default.
• W4319662956 abstract "Porcine astrovirus (PAstV) is a common cause of diarrhea in swine farms. The current understanding of the molecular virology and pathogenesis of PAstV is incomplete, especially due to the limited functional tools available. Here, ten sites in the open reading frame 1b (ORF1b) of the PAstV genome were determined to tolerate random 15 nt insertions based on the infectious full-length cDNA clones of PAstV using transposon-based insertion-mediated mutagenesis of three selected regions of the PAstV genome. Insertion of the commonly used Flag tag into seven of the ten insertion sites allowed the production of infectious viruses and allowed their recognition by specifically labeled monoclonal antibodies. Indirect immunofluorescence showed that the Flag-tagged ORF1b protein partially overlapped with the coat protein within the cytoplasm. An improved light-oxygen-voltage (iLOV) gene was also introduced into these seven sites, and only one viable recombinant virus that expressed the iLOV reporter gene at the B2 site was recovered. Biological analysis of the reporter viruses showed that these exhibited similar growth characteristics to the parental virus, but they produced fewer infectious virus particles and replicated at a slower rate. The recombinant viruses containing iLOV fused to ORF1b protein, which maintained their stability and displayed green fluorescence for up to three generations after passaging in cell culture. The porcine astroviruses (PAstVs) expressing iLOV were then used to assess the in vitro antiviral activities of mefloquine hydrochloride and ribavirin. Altogether, the recombinant PAstVs expressing iLOV can be used as a reporter virus tool for the screening of anti-PAstV drugs as well as the investigation of PAstV replication and the functional activities of proteins in living cells." @default.
• W4319662956 created "2023-02-10" @default.
• W4319662956 creator A5007767899 @default.
• W4319662956 creator A5038516394 @default.
• W4319662956 creator A5040558103 @default.
• W4319662956 creator A5042389015 @default.
• W4319662956 creator A5054034850 @default.
• W4319662956 creator A5059623439 @default.
• W4319662956 creator A5061671556 @default.
• W4319662956 creator A5078399013 @default.
• W4319662956 creator A5083148043 @default.
• W4319662956 creator A5083998403 @default.
• W4319662956 creator A5087857684 @default.
• W4319662956 date "2023-05-01" @default.
• W4319662956 modified "2023-10-10" @default.
• W4319662956 title "Insertion of exogenous genes within the ORF1b coding region of porcine astrovirus" @default.
• W4319662956 cites W1968679635 @default.
• W4319662956 cites W1980783855 @default.
• W4319662956 cites W1982326515 @default.
• W4319662956 cites W1987465441 @default.
• W4319662956 cites W2018346120 @default.
• W4319662956 cites W2026988878 @default.
• W4319662956 cites W2101624835 @default.
• W4319662956 cites W2102373548 @default.
• W4319662956 cites W2146211159 @default.
• W4319662956 cites W2482733453 @default.
• W4319662956 cites W2762528019 @default.
• W4319662956 cites W2789468721 @default.
• W4319662956 cites W2910548676 @default.
• W4319662956 cites W2947966600 @default.
• W4319662956 cites W2972270555 @default.
• W4319662956 cites W3030144725 @default.
• W4319662956 cites W3048725132 @default.
• W4319662956 cites W3094200731 @default.
• W4319662956 cites W3120267746 @default.
• W4319662956 cites W4211204104 @default.
• W4319662956 cites W4220795792 @default.
• W4319662956 doi "https://doi.org/10.1016/j.vetmic.2023.109675" @default.
• W4319662956 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36812864" @default.
• W4319662956 hasPublicationYear "2023" @default.
• W4319662956 type Work @default.
• W4319662956 citedByCount "1" @default.
• W4319662956 countsByYear W43196629562023 @default.
• W4319662956 crossrefType "journal-article" @default.
• W4319662956 hasAuthorship W4319662956A5007767899 @default.
• W4319662956 hasAuthorship W4319662956A5038516394 @default.
• W4319662956 hasAuthorship W4319662956A5040558103 @default.
• W4319662956 hasAuthorship W4319662956A5042389015 @default.
• W4319662956 hasAuthorship W4319662956A5054034850 @default.
• W4319662956 hasAuthorship W4319662956A5059623439 @default.
• W4319662956 hasAuthorship W4319662956A5061671556 @default.
• W4319662956 hasAuthorship W4319662956A5078399013 @default.
• W4319662956 hasAuthorship W4319662956A5083148043 @default.
• W4319662956 hasAuthorship W4319662956A5083998403 @default.
• W4319662956 hasAuthorship W4319662956A5087857684 @default.
• W4319662956 hasConcept C104317684 @default.
• W4319662956 hasConcept C141231307 @default.
• W4319662956 hasConcept C153911025 @default.
• W4319662956 hasConcept C159047783 @default.
• W4319662956 hasConcept C187882448 @default.
• W4319662956 hasConcept C2522874641 @default.
• W4319662956 hasConcept C40767141 @default.
• W4319662956 hasConcept C54355233 @default.
• W4319662956 hasConcept C86803240 @default.
• W4319662956 hasConceptScore W4319662956C104317684 @default.
• W4319662956 hasConceptScore W4319662956C141231307 @default.
• W4319662956 hasConceptScore W4319662956C153911025 @default.
• W4319662956 hasConceptScore W4319662956C159047783 @default.
• W4319662956 hasConceptScore W4319662956C187882448 @default.
• W4319662956 hasConceptScore W4319662956C2522874641 @default.
• W4319662956 hasConceptScore W4319662956C40767141 @default.
• W4319662956 hasConceptScore W4319662956C54355233 @default.
• W4319662956 hasConceptScore W4319662956C86803240 @default.
• W4319662956 hasFunder F4320321001 @default.
• W4319662956 hasLocation W43196629561 @default.
• W4319662956 hasLocation W43196629562 @default.
• W4319662956 hasOpenAccess W4319662956 @default.
• W4319662956 hasPrimaryLocation W43196629561 @default.
• W4319662956 hasRelatedWork W1491897707 @default.
• W4319662956 hasRelatedWork W2058443900 @default.
• W4319662956 hasRelatedWork W2107405807 @default.
• W4319662956 hasRelatedWork W2138721420 @default.
• W4319662956 hasRelatedWork W2266553821 @default.
• W4319662956 hasRelatedWork W2274607931 @default.
• W4319662956 hasRelatedWork W2367910728 @default.
• W4319662956 hasRelatedWork W2987420353 @default.
• W4319662956 hasRelatedWork W3028970346 @default.
• W4319662956 hasRelatedWork W4206192742 @default.
• W4319662956 hasVolume "280" @default.
• W4319662956 isParatext "false" @default.
• W4319662956 isRetracted "false" @default.
• W4319662956 workType "article" @default.