FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4319724539> ?p ?o ?g. }

• W4319724539 abstract "Abstract Protein-protein interactions (PPIs) regulate many cellular processes, and engineered PPIs have cell and gene therapy applications. Here we introduce massively parallel protein-protein interaction measurement by sequencing (MP3-seq), an easy-to-use and highly scalable yeast-two-hybrid approach for measuring PPIs. In MP3-seq, DNA barcodes are associated with specific protein pairs, and barcode enrichment can be read by sequencing to provide a direct measure of interaction strength. We show that MP3-seq is highly quantitative and scales to over 100,000 interactions. We apply MP3-seq to characterize interactions between families of rationally designed heterodimers and to investigate elements conferring specificity to coiled-coil interactions. Finally, we predict coiled heterodimer structures using AlphaFold-Multimer (AF-M) and train linear models on physics simulation energy terms to predict MP3-seq values. We find that AF-M and AF-M complex prediction-based models could be valuable for pre-screening interactions, but that measuring interactions experimentally remains necessary to rank their strengths quantitatively." @default.
• W4319724539 created "2023-02-11" @default.
• W4319724539 creator A5010584070 @default.
• W4319724539 creator A5011037544 @default.
• W4319724539 creator A5021731745 @default.
• W4319724539 creator A5028123601 @default.
• W4319724539 creator A5049578097 @default.
• W4319724539 creator A5054919302 @default.
• W4319724539 creator A5056258617 @default.
• W4319724539 date "2023-02-09" @default.
• W4319724539 modified "2023-10-17" @default.
• W4319724539 title "Massively parallel protein-protein interaction measurement by sequencing (MP3-seq) enables rapid screening of protein heterodimers" @default.
• W4319724539 cites W1505986412 @default.
• W4319724539 cites W1823814103 @default.
• W4319724539 cites W1977186059 @default.
• W4319724539 cites W1990315216 @default.
• W4319724539 cites W2008295579 @default.
• W4319724539 cites W2009019945 @default.
• W4319724539 cites W2036897871 @default.
• W4319724539 cites W2053983828 @default.
• W4319724539 cites W2079760969 @default.
• W4319724539 cites W2084493621 @default.
• W4319724539 cites W2084611577 @default.
• W4319724539 cites W2097210639 @default.
• W4319724539 cites W2107409328 @default.
• W4319724539 cites W2107532817 @default.
• W4319724539 cites W212599732 @default.
• W4319724539 cites W2128323824 @default.
• W4319724539 cites W2134900594 @default.
• W4319724539 cites W2138141821 @default.
• W4319724539 cites W2160677068 @default.
• W4319724539 cites W2179438025 @default.
• W4319724539 cites W2298224298 @default.
• W4319724539 cites W2313226053 @default.
• W4319724539 cites W2339738230 @default.
• W4319724539 cites W2345914382 @default.
• W4319724539 cites W2474853622 @default.
• W4319724539 cites W2513154881 @default.
• W4319724539 cites W2546846550 @default.
• W4319724539 cites W2656283085 @default.
• W4319724539 cites W2735621019 @default.
• W4319724539 cites W2735648988 @default.
• W4319724539 cites W2765373652 @default.
• W4319724539 cites W2766322286 @default.
• W4319724539 cites W2769889167 @default.
• W4319724539 cites W2781028774 @default.
• W4319724539 cites W2790876771 @default.
• W4319724539 cites W2797104734 @default.
• W4319724539 cites W2800187318 @default.
• W4319724539 cites W2890807572 @default.
• W4319724539 cites W2904947448 @default.
• W4319724539 cites W2906060794 @default.
• W4319724539 cites W2943920837 @default.
• W4319724539 cites W2950464686 @default.
• W4319724539 cites W2997938055 @default.
• W4319724539 cites W3014096229 @default.
• W4319724539 cites W3015964336 @default.
• W4319724539 cites W3098028732 @default.
• W4319724539 cites W3116040669 @default.
• W4319724539 cites W3120712987 @default.
• W4319724539 cites W3134805435 @default.
• W4319724539 cites W3136708714 @default.
• W4319724539 cites W3142310073 @default.
• W4319724539 cites W3153508581 @default.
• W4319724539 cites W3159270195 @default.
• W4319724539 cites W3177828909 @default.
• W4319724539 cites W3186179742 @default.
• W4319724539 cites W4249558460 @default.
• W4319724539 cites W4281790889 @default.
• W4319724539 cites W4286500588 @default.
• W4319724539 cites W4290473117 @default.
• W4319724539 cites W4297235560 @default.
• W4319724539 cites W4309588718 @default.
• W4319724539 cites W4327550249 @default.
• W4319724539 cites W4385483399 @default.
• W4319724539 doi "https://doi.org/10.1101/2023.02.08.527770" @default.
• W4319724539 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36798377" @default.
• W4319724539 hasPublicationYear "2023" @default.
• W4319724539 type Work @default.
• W4319724539 citedByCount "0" @default.
• W4319724539 crossrefType "posted-content" @default.
• W4319724539 hasAuthorship W4319724539A5010584070 @default.
• W4319724539 hasAuthorship W4319724539A5011037544 @default.
• W4319724539 hasAuthorship W4319724539A5021731745 @default.
• W4319724539 hasAuthorship W4319724539A5028123601 @default.
• W4319724539 hasAuthorship W4319724539A5049578097 @default.
• W4319724539 hasAuthorship W4319724539A5054919302 @default.
• W4319724539 hasAuthorship W4319724539A5056258617 @default.
• W4319724539 hasBestOaLocation W43197245391 @default.
• W4319724539 hasConcept C104317684 @default.
• W4319724539 hasConcept C111919701 @default.
• W4319724539 hasConcept C11804247 @default.
• W4319724539 hasConcept C173608175 @default.
• W4319724539 hasConcept C186060115 @default.
• W4319724539 hasConcept C190475519 @default.
• W4319724539 hasConcept C192800701 @default.
• W4319724539 hasConcept C2776841711 @default.
• W4319724539 hasConcept C41008148 @default.
• W4319724539 hasConcept C48044578 @default.
• W4319724539 hasConcept C51679486 @default.

• next