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• W4319737113 abstract "Genetic selection is important in choosing animals for the next generation. Animal breeders now choose selection candidates based on DNA markers, in addition to animal performance. There has been increased use of high-density SNP chips to enable selection of commercial broiler and layer chickens. However, genotyping a large number of selection candidates still carries a high cost. The aim of our study was to develop an economical 5K SNP panel for local Ghanian and Tanzanian chicken ecotypes using targeted genotyping by sequencing (GBS) for imputation to higher density. Low-density panel SNPs were selected from haplotype blocks and other important genomic regions across the chicken genome based on 600K SNP panel genotypes. A total of 188 birds were genotyped by GBS and an in-house shell script pipeline was utilized to obtain SNP calls. Our in-house pipeline was compared to a standard company pipeline and 600K SNP chip genotypes for validation. Selected SNPs were evenly distributed across the genome, with at least one SNP in each megabase region. Comparison of the two pipelines revealed a good genotype match for the 5K SNP panel. The 5K GBS panel and SNP calling pipeline are important tools to aid selective breeding in African chicken ecotypes." @default.
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• W4319737113 date "2022-12-31" @default.
• W4319737113 modified "2023-09-30" @default.
• W4319737113 title "461. Development of a low-density SNP panel for local Ghanaian and Tanzanian chicken ecotypes" @default.
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• W4319737113 doi "https://doi.org/10.3920/978-90-8686-940-4_461" @default.
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