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- W4319868824 abstract "In this study, we introduced H-terminated diamond solution-gate field-effect transistor (H-diamond SGFET) to detect trace SARS-CoV-2 N-protein, which plays an important role in replication and transcription of viral RNA. 1-Pyrenebutyric acid-N-hydroxy succinimide ester (Pyr-NHS) was modified on H-diamond surface as linker, on which the specific antibody of SARS-CoV-2 N-protein was catenated. Fourier transform infrared spectrum, scanning electron microscope and energy dispersive spectrum were utilized to demonstrate the modification of H-diamond with Pyr-NHS and antibody. Shifts of IDS(max) at VGS = −500 mV in transfer characteristics of H-diamond SGFET was observed to determine N-protein concentration in phosphate buffer solution. Good linear relationship between IDS(max) and log10(N-protein) was observed from 10−14 to 10−5 g/mL with goodness of fit R2 = 0.90 and sensitivity of 1.98 μA/Log10 [concentration of N-protein] at VDS = −500 mV, VGS = −500 mV. Consequently, this prepared H-diamond SGFET biosensor may provide a new idea for diagnosis of SARS-CoV-2 due to a wide detection range from 10−14 to 10−5 g/mL and low limit of detection 10−14 g/mL." @default.
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- W4319868824 date "2023-04-01" @default.
- W4319868824 modified "2023-10-18" @default.
- W4319868824 title "Trace detection of SARS-CoV-2 N-protein by diamond solution-gate field-effect transistor" @default.
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- W4319868824 doi "https://doi.org/10.1016/j.diamond.2023.109775" @default.
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