Matches in SemOpenAlex for { <https://semopenalex.org/work/W4319870169> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W4319870169 endingPage "205a" @default.
- W4319870169 startingPage "205a" @default.
- W4319870169 abstract "RNA viruses hijack host cell functions to promote viral replication. During infection, viral components enter membrane-bound and membrane-less cellular organelles, including the phase-separated nucleolus, where they perturb nucleolar proteins to aid virus replication. Recently, viruses have been shown to utilize the process of liquid-liquid phase separation (LLPS) to package their genome and promote viral RNA synthesis. The viral nucleocapsid (N) protein is critically involved in several aspects of virus replication. Importantly, the nucleolar phosphoprotein, Nucleophosmin (NPM1), with roles in ribosome biogenesis, was reported to interact with the N protein of several different RNA viruses and facilitate viral replication. However, the impact of this interaction and SARS CoV-2 N protein (NCoV-2) nucleolar localization on ribosome biogenesis and viral life cycle remains poorly understood. Our in vitro studies show that NPM1 undergoes heterotypic phase separation with NCoV-2, causing the formation of liquid-like droplets or condensates with restricted NPM1 mobility. Sequence analysis of NCoV-2 revealed enrichment of positively charged (Arg and Lys) amino acids, an important “sequence feature” that governs nuclear and nucleolar localization. Importantly, our cellular studies reveal the colocalization of NCoV-2 with NPM1 in the nucleoli of Vero E6 cells. These results suggest that there is a direct interaction between NPM1 and NCoV-2. Strikingly, NCoV-2 displaces NPM1 from reconstituted, granular component-like condensates and from nucleoli. Based on our collective findings, we propose that displacement of regulatory proteins (e.g., NPM1) from nucleoli by NCoV-2 through interactions of its Arg residues with ribosomal RNA (rRNA), may promote protein synthesis and the viral life cycle. These mechanistic insights will provide new avenues for the development of novel antiviral therapies for inhibition of nucleolar localization of NCoV-2." @default.
- W4319870169 created "2023-02-11" @default.
- W4319870169 creator A5015019969 @default.
- W4319870169 creator A5039651149 @default.
- W4319870169 creator A5048145620 @default.
- W4319870169 creator A5054720253 @default.
- W4319870169 creator A5075145095 @default.
- W4319870169 creator A5090871845 @default.
- W4319870169 date "2023-02-01" @default.
- W4319870169 modified "2023-09-24" @default.
- W4319870169 title "Phase separation underlies interactions of the SARS-CoV-2 nucleocapsid protein with nucleoli" @default.
- W4319870169 doi "https://doi.org/10.1016/j.bpj.2022.11.1234" @default.
- W4319870169 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36782995" @default.
- W4319870169 hasPublicationYear "2023" @default.
- W4319870169 type Work @default.
- W4319870169 citedByCount "0" @default.
- W4319870169 crossrefType "journal-article" @default.
- W4319870169 hasAuthorship W4319870169A5015019969 @default.
- W4319870169 hasAuthorship W4319870169A5039651149 @default.
- W4319870169 hasAuthorship W4319870169A5048145620 @default.
- W4319870169 hasAuthorship W4319870169A5054720253 @default.
- W4319870169 hasAuthorship W4319870169A5075145095 @default.
- W4319870169 hasAuthorship W4319870169A5090871845 @default.
- W4319870169 hasBestOaLocation W43198701692 @default.
- W4319870169 hasConcept C119857082 @default.
- W4319870169 hasConcept C12554922 @default.
- W4319870169 hasConcept C142724271 @default.
- W4319870169 hasConcept C159047783 @default.
- W4319870169 hasConcept C178790620 @default.
- W4319870169 hasConcept C183873130 @default.
- W4319870169 hasConcept C185592680 @default.
- W4319870169 hasConcept C190062978 @default.
- W4319870169 hasConcept C2776061190 @default.
- W4319870169 hasConcept C2779134260 @default.
- W4319870169 hasConcept C3007834351 @default.
- W4319870169 hasConcept C3008058167 @default.
- W4319870169 hasConcept C41008148 @default.
- W4319870169 hasConcept C44280652 @default.
- W4319870169 hasConcept C524204448 @default.
- W4319870169 hasConcept C70721500 @default.
- W4319870169 hasConcept C71924100 @default.
- W4319870169 hasConcept C86803240 @default.
- W4319870169 hasConcept C95444343 @default.
- W4319870169 hasConceptScore W4319870169C119857082 @default.
- W4319870169 hasConceptScore W4319870169C12554922 @default.
- W4319870169 hasConceptScore W4319870169C142724271 @default.
- W4319870169 hasConceptScore W4319870169C159047783 @default.
- W4319870169 hasConceptScore W4319870169C178790620 @default.
- W4319870169 hasConceptScore W4319870169C183873130 @default.
- W4319870169 hasConceptScore W4319870169C185592680 @default.
- W4319870169 hasConceptScore W4319870169C190062978 @default.
- W4319870169 hasConceptScore W4319870169C2776061190 @default.
- W4319870169 hasConceptScore W4319870169C2779134260 @default.
- W4319870169 hasConceptScore W4319870169C3007834351 @default.
- W4319870169 hasConceptScore W4319870169C3008058167 @default.
- W4319870169 hasConceptScore W4319870169C41008148 @default.
- W4319870169 hasConceptScore W4319870169C44280652 @default.
- W4319870169 hasConceptScore W4319870169C524204448 @default.
- W4319870169 hasConceptScore W4319870169C70721500 @default.
- W4319870169 hasConceptScore W4319870169C71924100 @default.
- W4319870169 hasConceptScore W4319870169C86803240 @default.
- W4319870169 hasConceptScore W4319870169C95444343 @default.
- W4319870169 hasIssue "3" @default.
- W4319870169 hasLocation W43198701691 @default.
- W4319870169 hasLocation W43198701692 @default.
- W4319870169 hasLocation W43198701693 @default.
- W4319870169 hasOpenAccess W4319870169 @default.
- W4319870169 hasPrimaryLocation W43198701691 @default.
- W4319870169 hasRelatedWork W1421917291 @default.
- W4319870169 hasRelatedWork W2026143593 @default.
- W4319870169 hasRelatedWork W2029209415 @default.
- W4319870169 hasRelatedWork W2066749872 @default.
- W4319870169 hasRelatedWork W2523760427 @default.
- W4319870169 hasRelatedWork W3127693599 @default.
- W4319870169 hasRelatedWork W4205317059 @default.
- W4319870169 hasRelatedWork W4206669628 @default.
- W4319870169 hasRelatedWork W4210401150 @default.
- W4319870169 hasRelatedWork W4385264226 @default.
- W4319870169 hasVolume "122" @default.
- W4319870169 isParatext "false" @default.
- W4319870169 isRetracted "false" @default.
- W4319870169 workType "article" @default.