Matches in SemOpenAlex for { <https://semopenalex.org/work/W4319872922> ?p ?o ?g. }
Showing items 1 to 58 of
58
with 100 items per page.
- W4319872922 endingPage "3a" @default.
- W4319872922 startingPage "3a" @default.
- W4319872922 abstract "The clustered regularly interspaced short palindromic repeat (CRISPR) genome-editing revolution established the beginning of a new era in life sciences. I will report the role of state-of-the-art computations in the CRISPR-Cas9 revolution, from the early refinement of cryo-EM data to enhanced simulations of large-scale conformational transitions. Molecular simulations reported a mechanism for RNA binding and the formation of a catalytically competent Cas9 enzyme, corroborated by recent cryo-EM data of the activated state. Inspired by single-molecule experiments, molecular dynamics offered a rationale for the onset of off-target effects, while graph theory unveiled the allosteric regulation. Finally, the use of a mixed quantum-classical approach established the catalytic mechanism of DNA cleavage. Overall, molecular simulations have been instrumental in understanding the dynamics and mechanism of CRISPR-Cas9, contributing to understanding function, catalysis, allostery, and specificity. Future computational studies will bridge the scales from quantum mechanical approaches to enhanced simulations and cryo-EM processing methods to push the boundaries of molecular simulations and tackle the most exciting problems in the biophysics of genome editing." @default.
- W4319872922 created "2023-02-11" @default.
- W4319872922 creator A5064887398 @default.
- W4319872922 date "2023-02-01" @default.
- W4319872922 modified "2023-09-25" @default.
- W4319872922 title "Theory and computation early career award talk: unraveling the CRISPR-Cas revolution through the lens of computational biophysics" @default.
- W4319872922 doi "https://doi.org/10.1016/j.bpj.2022.11.251" @default.
- W4319872922 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36784042" @default.
- W4319872922 hasPublicationYear "2023" @default.
- W4319872922 type Work @default.
- W4319872922 citedByCount "0" @default.
- W4319872922 crossrefType "journal-article" @default.
- W4319872922 hasAuthorship W4319872922A5064887398 @default.
- W4319872922 hasConcept C104317684 @default.
- W4319872922 hasConcept C120665830 @default.
- W4319872922 hasConcept C121332964 @default.
- W4319872922 hasConcept C15336307 @default.
- W4319872922 hasConcept C15744967 @default.
- W4319872922 hasConcept C188147891 @default.
- W4319872922 hasConcept C199360897 @default.
- W4319872922 hasConcept C41008148 @default.
- W4319872922 hasConcept C45374587 @default.
- W4319872922 hasConcept C54355233 @default.
- W4319872922 hasConcept C86803240 @default.
- W4319872922 hasConcept C98108389 @default.
- W4319872922 hasConceptScore W4319872922C104317684 @default.
- W4319872922 hasConceptScore W4319872922C120665830 @default.
- W4319872922 hasConceptScore W4319872922C121332964 @default.
- W4319872922 hasConceptScore W4319872922C15336307 @default.
- W4319872922 hasConceptScore W4319872922C15744967 @default.
- W4319872922 hasConceptScore W4319872922C188147891 @default.
- W4319872922 hasConceptScore W4319872922C199360897 @default.
- W4319872922 hasConceptScore W4319872922C41008148 @default.
- W4319872922 hasConceptScore W4319872922C45374587 @default.
- W4319872922 hasConceptScore W4319872922C54355233 @default.
- W4319872922 hasConceptScore W4319872922C86803240 @default.
- W4319872922 hasConceptScore W4319872922C98108389 @default.
- W4319872922 hasIssue "3" @default.
- W4319872922 hasLocation W43198729221 @default.
- W4319872922 hasLocation W43198729222 @default.
- W4319872922 hasOpenAccess W4319872922 @default.
- W4319872922 hasPrimaryLocation W43198729221 @default.
- W4319872922 hasRelatedWork W1572523360 @default.
- W4319872922 hasRelatedWork W177283563 @default.
- W4319872922 hasRelatedWork W2405656580 @default.
- W4319872922 hasRelatedWork W2642604616 @default.
- W4319872922 hasRelatedWork W2748952813 @default.
- W4319872922 hasRelatedWork W2899084033 @default.
- W4319872922 hasRelatedWork W3162049976 @default.
- W4319872922 hasRelatedWork W4293199045 @default.
- W4319872922 hasRelatedWork W4321259174 @default.
- W4319872922 hasRelatedWork W3129737589 @default.
- W4319872922 hasVolume "122" @default.
- W4319872922 isParatext "false" @default.
- W4319872922 isRetracted "false" @default.
- W4319872922 workType "article" @default.