Matches in SemOpenAlex for { <https://semopenalex.org/work/W4319930140> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W4319930140 endingPage "231a" @default.
- W4319930140 startingPage "231a" @default.
- W4319930140 abstract "Membrane proteins regulate several vital cellular processes, establishing them as major drug targets. A main function of membrane proteins is to transduce extracellular signal across the membrane bilayer. One such protein is the epidermal growth factor receptor (EGFR), which plays a crucial role in cell signaling and is implicated in cancer. While the structures of the extracellular and intracellular regions of this protein have been well-elucidated individually, conformational coupling connecting these two regions during signal transduction is challenging to probe due to the mixture of hydrophobic and hydrophilic domains intrinsic to membrane proteins. Here, I will discuss the signal transduction mechanism across full-length EGFR. We combine two biochemical tools, cell-free protein expression and nanodiscs, to isolate full-length, functional EGFR in a near-physiological environment. Using a multidisciplinary approach involving single-molecule Förster resonance energy transfer, mutagenesis, and molecular dynamics simulations, we observe a compaction in the intracellular domain of EGFR upon extracellular ligand binding in a neutral lipid environment. The ligand-induced extracellular/intracellular conformational coupling is reversed in the presence of anionic lipids, robust around the physiological 15-30% anionic content, and disappears in the presence of cholesterol. Our findings show how the extracellular and intracellular domains are coupled to each other in this critical receptor and the impact of the lipid composition in this conformational coupling. Our results could be universal to other membrane receptors which share the same structural homology and perform other significant functions." @default.
- W4319930140 created "2023-02-11" @default.
- W4319930140 creator A5015529735 @default.
- W4319930140 creator A5021471823 @default.
- W4319930140 creator A5023068548 @default.
- W4319930140 creator A5032132306 @default.
- W4319930140 creator A5046672665 @default.
- W4319930140 creator A5046881277 @default.
- W4319930140 creator A5053715204 @default.
- W4319930140 creator A5053781246 @default.
- W4319930140 creator A5078250888 @default.
- W4319930140 creator A5082234024 @default.
- W4319930140 creator A5090919441 @default.
- W4319930140 date "2023-02-01" @default.
- W4319930140 modified "2023-09-30" @default.
- W4319930140 title "Lipid dependence of the conformational coupling across the membrane bilayer of full-length epidermal growth factor receptor" @default.
- W4319930140 doi "https://doi.org/10.1016/j.bpj.2022.11.1362" @default.
- W4319930140 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36783137" @default.
- W4319930140 hasPublicationYear "2023" @default.
- W4319930140 type Work @default.
- W4319930140 citedByCount "0" @default.
- W4319930140 crossrefType "journal-article" @default.
- W4319930140 hasAuthorship W4319930140A5015529735 @default.
- W4319930140 hasAuthorship W4319930140A5021471823 @default.
- W4319930140 hasAuthorship W4319930140A5023068548 @default.
- W4319930140 hasAuthorship W4319930140A5032132306 @default.
- W4319930140 hasAuthorship W4319930140A5046672665 @default.
- W4319930140 hasAuthorship W4319930140A5046881277 @default.
- W4319930140 hasAuthorship W4319930140A5053715204 @default.
- W4319930140 hasAuthorship W4319930140A5053781246 @default.
- W4319930140 hasAuthorship W4319930140A5078250888 @default.
- W4319930140 hasAuthorship W4319930140A5082234024 @default.
- W4319930140 hasAuthorship W4319930140A5090919441 @default.
- W4319930140 hasConcept C12554922 @default.
- W4319930140 hasConcept C131584629 @default.
- W4319930140 hasConcept C159467904 @default.
- W4319930140 hasConcept C185592680 @default.
- W4319930140 hasConcept C191897082 @default.
- W4319930140 hasConcept C192157962 @default.
- W4319930140 hasConcept C192562407 @default.
- W4319930140 hasConcept C39944091 @default.
- W4319930140 hasConcept C41625074 @default.
- W4319930140 hasConcept C55493867 @default.
- W4319930140 hasConcept C8010536 @default.
- W4319930140 hasConcept C86803240 @default.
- W4319930140 hasConceptScore W4319930140C12554922 @default.
- W4319930140 hasConceptScore W4319930140C131584629 @default.
- W4319930140 hasConceptScore W4319930140C159467904 @default.
- W4319930140 hasConceptScore W4319930140C185592680 @default.
- W4319930140 hasConceptScore W4319930140C191897082 @default.
- W4319930140 hasConceptScore W4319930140C192157962 @default.
- W4319930140 hasConceptScore W4319930140C192562407 @default.
- W4319930140 hasConceptScore W4319930140C39944091 @default.
- W4319930140 hasConceptScore W4319930140C41625074 @default.
- W4319930140 hasConceptScore W4319930140C55493867 @default.
- W4319930140 hasConceptScore W4319930140C8010536 @default.
- W4319930140 hasConceptScore W4319930140C86803240 @default.
- W4319930140 hasIssue "3" @default.
- W4319930140 hasLocation W43199301401 @default.
- W4319930140 hasLocation W43199301402 @default.
- W4319930140 hasOpenAccess W4319930140 @default.
- W4319930140 hasPrimaryLocation W43199301401 @default.
- W4319930140 hasRelatedWork W1993362267 @default.
- W4319930140 hasRelatedWork W2000861222 @default.
- W4319930140 hasRelatedWork W2013928131 @default.
- W4319930140 hasRelatedWork W2020705112 @default.
- W4319930140 hasRelatedWork W2024914238 @default.
- W4319930140 hasRelatedWork W2081023259 @default.
- W4319930140 hasRelatedWork W2132850870 @default.
- W4319930140 hasRelatedWork W2346848543 @default.
- W4319930140 hasRelatedWork W2604676471 @default.
- W4319930140 hasRelatedWork W2791260019 @default.
- W4319930140 hasVolume "122" @default.
- W4319930140 isParatext "false" @default.
- W4319930140 isRetracted "false" @default.
- W4319930140 workType "article" @default.