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- W4319968048 endingPage "496" @default.
- W4319968048 startingPage "496" @default.
- W4319968048 abstract "Numerous proteomic and transcriptomic studies have been carried out to better understand the current multi-variant SARS-CoV-2 virus mechanisms of action and effects. However, they are mostly centered on mRNAs and proteins. The effect of the virus on human post-transcriptional regulatory agents such as microRNAs (miRNAs), which are involved in the regulation of 60% of human gene activity, remains poorly explored. Similar to research we have previously undertaken with other viruses such as Ebola and HIV, in this study we investigated the miRNA profile of lung epithelial cells following infection with SARS-CoV-2. At the 24 and 72 h post-infection time points, SARS-CoV-2 did not drastically alter the miRNome. About 90% of the miRNAs remained non-differentially expressed. The results revealed that miR-1246, miR-1290 and miR-4728-5p were the most upregulated over time. miR-196b-5p and miR-196a-5p were the most downregulated at 24 h, whereas at 72 h, miR-3924, miR-30e-5p and miR-145-3p showed the highest level of downregulation. In the top significantly enriched KEGG pathways of genes targeted by differentially expressed miRNAs we found, among others, MAPK, RAS, P13K-Akt and renin secretion signaling pathways. Using RT-qPCR, we also showed that SARS-CoV-2 may regulate several predicted host mRNA targets involved in the entry of the virus into host cells (ACE2, TMPRSS2, ADAM17, FURIN), renin-angiotensin system (RAS) (Renin, Angiotensinogen, ACE), innate immune response (IL-6, IFN1β, CXCL10, SOCS4) and fundamental cellular processes (AKT, NOTCH, WNT). Finally, we demonstrated by dual-luciferase assay a direct interaction between miR-1246 and ACE-2 mRNA. This study highlights the modulatory role of miRNAs in the pathogenesis of SARS-CoV-2." @default.
- W4319968048 created "2023-02-11" @default.
- W4319968048 creator A5003839046 @default.
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- W4319968048 creator A5076939191 @default.
- W4319968048 creator A5078849862 @default.
- W4319968048 creator A5080106827 @default.
- W4319968048 date "2023-02-10" @default.
- W4319968048 modified "2023-09-26" @default.
- W4319968048 title "Altered microRNA Transcriptome in Cultured Human Airway Cells upon Infection with SARS-CoV-2" @default.
- W4319968048 cites W1969963419 @default.
- W4319968048 cites W1975319407 @default.
- W4319968048 cites W1984815607 @default.
- W4319968048 cites W1996703890 @default.
- W4319968048 cites W2025299909 @default.
- W4319968048 cites W2026570544 @default.
- W4319968048 cites W2027149423 @default.
- W4319968048 cites W2032019813 @default.
- W4319968048 cites W2041935553 @default.
- W4319968048 cites W2046153984 @default.
- W4319968048 cites W2057100799 @default.
- W4319968048 cites W2094875270 @default.
- W4319968048 cites W2103017472 @default.
- W4319968048 cites W2107277218 @default.
- W4319968048 cites W2113642147 @default.
- W4319968048 cites W2134250297 @default.
- W4319968048 cites W2139273728 @default.
- W4319968048 cites W2144038313 @default.
- W4319968048 cites W2146079676 @default.
- W4319968048 cites W2159482845 @default.
- W4319968048 cites W2160063406 @default.
- W4319968048 cites W2171296186 @default.
- W4319968048 cites W2192080449 @default.
- W4319968048 cites W2255557258 @default.
- W4319968048 cites W2288117658 @default.
- W4319968048 cites W2470277042 @default.
- W4319968048 cites W2563350820 @default.
- W4319968048 cites W2799609390 @default.
- W4319968048 cites W2950595506 @default.
- W4319968048 cites W2969587497 @default.
- W4319968048 cites W2984641466 @default.
- W4319968048 cites W3001041847 @default.
- W4319968048 cites W3009335299 @default.
- W4319968048 cites W3009424226 @default.
- W4319968048 cites W3009912996 @default.
- W4319968048 cites W3014662258 @default.
- W4319968048 cites W3014941429 @default.
- W4319968048 cites W3017302293 @default.
- W4319968048 cites W3021577619 @default.
- W4319968048 cites W3025847192 @default.
- W4319968048 cites W3030871984 @default.
- W4319968048 cites W3035985313 @default.
- W4319968048 cites W3036881316 @default.
- W4319968048 cites W3040779579 @default.
- W4319968048 cites W3040907131 @default.
- W4319968048 cites W3042094126 @default.
- W4319968048 cites W3048490071 @default.
- W4319968048 cites W3049068517 @default.
- W4319968048 cites W3082736053 @default.
- W4319968048 cites W3087149315 @default.
- W4319968048 cites W3088503615 @default.
- W4319968048 cites W3092393238 @default.
- W4319968048 cites W3092778802 @default.
- W4319968048 cites W3093061534 @default.
- W4319968048 cites W3095024243 @default.
- W4319968048 cites W3096670014 @default.
- W4319968048 cites W3104092741 @default.
- W4319968048 cites W3118438588 @default.
- W4319968048 cites W3119599233 @default.
- W4319968048 cites W3121790907 @default.
- W4319968048 cites W3124723126 @default.
- W4319968048 cites W3124805424 @default.
- W4319968048 cites W3128962620 @default.
- W4319968048 cites W3129717864 @default.
- W4319968048 cites W3134906456 @default.
- W4319968048 cites W3144727764 @default.
- W4319968048 cites W3147268564 @default.
- W4319968048 cites W3157337586 @default.
- W4319968048 cites W3158158224 @default.
- W4319968048 cites W3158741652 @default.
- W4319968048 cites W3167017974 @default.
- W4319968048 cites W3176842152 @default.
- W4319968048 cites W3178982155 @default.
- W4319968048 cites W3184442129 @default.
- W4319968048 cites W3206790845 @default.
- W4319968048 cites W4200231439 @default.
- W4319968048 cites W4205185259 @default.
- W4319968048 cites W4206395253 @default.
- W4319968048 cites W4206669294 @default.
- W4319968048 cites W4210705946 @default.
- W4319968048 cites W4211064852 @default.
- W4319968048 cites W4225701835 @default.
- W4319968048 cites W4226299584 @default.
- W4319968048 cites W4229332595 @default.
- W4319968048 cites W4240748016 @default.
- W4319968048 cites W4280541080 @default.
- W4319968048 cites W4281666631 @default.