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• W4319984816 abstract "Mastitis is a widespread mammary gland disease of dairy cows that causes severe economic losses to dairy farms. Mastitis can be caused by bacteria, fungi, and algae. The most common species isolated from infected milk are, among others, Streptococcus spp., and Escherichia coli. The aim of our study was protein detection based on both in silico and in vitro methods, which allowed the identification of immunoreactive proteins representative of the following species: Streptococcus uberis, Streptococcus agalactiae, and Escherichia coli.The study group included 22 milk samples and 13 serum samples obtained from cows with diagnosed mastitis, whereas the control group constituted 12 milk samples and 12 serum samples isolated from healthy animals. Detection of immunoreactive proteins was done by immunoblotting, while amino acid sequences from investigated proteins were determined by MALDI-TOF. Then, bioinformatic analyses were performed on detected species specific proteins in order to investigate their immunoreactivity.As a result, we identified 13 proteins: 3 (molybdenum cofactor biosynthesis protein B, aldehyde reductase YahK, outer membrane protein A) for E. coli, 4 (elongation factor Tu, tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG, GTPase Obg, glyceraldehyde-3-phosphate dehydrogenase) for S. uberis, and 6 (aspartate carbamoyltransferase, elongation factor Tu, 60 kDa chaperonin, elongation factor G, galactose-6-phosphate isomerase subunit LacA, adenosine deaminase) for S. agalactiae, which demonstrated immunoreactivity to antibodies present in serum from cows with diagnosed mastitis.Due to the confirmed immunoreactivity, specificity and localization in the bacterial cell, these proteins can be considered considered potential targets in innovative rapid immunodiagnostic assays for bovine mastitis, however due to the limited number of examined samples, further examination is needed." @default.
• W4319984816 created "2023-02-11" @default.
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• W4319984816 date "2023-02-10" @default.
• W4319984816 modified "2023-09-30" @default.
• W4319984816 title "Detection of immunoreactive proteins of Escherichia coli, Streptococcus uberis, and Streptococcus agalactiae isolated from cows with diagnosed mastitis" @default.
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• W4319984816 cites W1972284605 @default.
• W4319984816 cites W1983414515 @default.
• W4319984816 cites W1991761638 @default.
• W4319984816 cites W1994887352 @default.
• W4319984816 cites W2000166525 @default.
• W4319984816 cites W2006201402 @default.
• W4319984816 cites W2011272739 @default.
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• W4319984816 cites W2016973128 @default.
• W4319984816 cites W2018289835 @default.
• W4319984816 cites W2023301041 @default.
• W4319984816 cites W2027753593 @default.
• W4319984816 cites W2028373209 @default.
• W4319984816 cites W2044658561 @default.
• W4319984816 cites W2047898798 @default.
• W4319984816 cites W2050430115 @default.
• W4319984816 cites W2053509474 @default.
• W4319984816 cites W2053707749 @default.
• W4319984816 cites W2075127511 @default.
• W4319984816 cites W2086970202 @default.
• W4319984816 cites W2089262982 @default.
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• W4319984816 cites W2100837269 @default.
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• W4319984816 cites W2118200955 @default.
• W4319984816 cites W2122054271 @default.
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• W4319984816 cites W2162792752 @default.
• W4319984816 cites W2166067962 @default.
• W4319984816 cites W2196025561 @default.
• W4319984816 cites W2337007288 @default.
• W4319984816 cites W2402015124 @default.
• W4319984816 cites W2407857973 @default.
• W4319984816 cites W2411461061 @default.
• W4319984816 cites W2411969087 @default.
• W4319984816 cites W2415598463 @default.
• W4319984816 cites W2425004119 @default.
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• W4319984816 cites W2620480148 @default.
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• W4319984816 cites W2736932263 @default.
• W4319984816 cites W2753320915 @default.
• W4319984816 cites W2766911825 @default.
• W4319984816 cites W2775258386 @default.
• W4319984816 cites W2789642889 @default.
• W4319984816 cites W2801104030 @default.
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• W4319984816 cites W2898093767 @default.
• W4319984816 cites W2910889146 @default.
• W4319984816 cites W2912990441 @default.
• W4319984816 cites W2940347290 @default.
• W4319984816 cites W2998080240 @default.
• W4319984816 cites W3015980338 @default.
• W4319984816 cites W3090765028 @default.
• W4319984816 cites W3103240054 @default.
• W4319984816 cites W3156927920 @default.
• W4319984816 cites W3164040204 @default.
• W4319984816 cites W3174843794 @default.
• W4319984816 cites W3192643510 @default.
• W4319984816 cites W3200178511 @default.
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• W4319984816 doi "https://doi.org/10.3389/fcimb.2023.987842" @default.
• W4319984816 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36844415" @default.
• W4319984816 hasPublicationYear "2023" @default.
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