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• W4319993313 abstract "Cancer is a disease that infects humans from abnormal cells that form rapidly and spread to other parts of the body. Currently, research on breast cancer treatment is being carried out. This research utilizes the wisdom of local plants as herbal plants (Typhonium flagelliforme), which is very important in the health sector. The purpose of this study was to identify specific DNA fragments in the superior mutant plant and the wild type (Bogor accession as control plant) based on RAPD markers and sequence difference analysis in each sample. A total of 15 RAPD primers were used to optimize polymorphic primers on 12 clones of mutant plants, with seven superior mutants with high anticancer compounds (SAT) and four mutant clones that contained low anticancer compounds (SAR) with the control. Primary optimization obtained two primers OPC05 and OPB18, which provided an informative band pattern between superior clone bulk mutants containing high anticancer compounds (BSAT) and bulk mutants containing low anticancer compounds (BSAR), and the control. OPB18 primer was chosen for sequencing analysis because it has a more informative DNA fragment luminescence at a size of about 500 bp. The BSAT, BSAR, and the control sequences analysis based on the codon sequence for each segment showed a difference in size between 45-55 bp, 100-105 bp, 150-160 bp, 225-235 bp, 260-265 bp, 375-400 bp, 410-430 bp, and 450-455 bp. The control plant's sequence analysis is around 75% with the date palm species (Phoenix dactylifera). These results can be used to design specific primers for anticancer compounds in mutant plants." @default.
• W4319993313 created "2023-02-11" @default.
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• W4319993313 date "2023-01-01" @default.
• W4319993313 modified "2023-09-25" @default.
• W4319993313 title "Direct verification of bogor superior mutant (Typhonium flagelliforme) using RAPD marker techniques and sequencing analysis" @default.
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• W4319993313 doi "https://doi.org/10.1063/5.0104057" @default.
• W4319993313 hasPublicationYear "2023" @default.
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