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- W4320032634 abstract "To investigate the expression and its relative mechanism of hypoxia-inducible factor-1α(HIF-1α) in bone marrow(BM) of mice during G-CSF mobilization of hematopoietic stem cells (HSC) .Flow cytometry was used to detect the proportion of Lin-Sca-1+ c-kit+ (LSK) cells in peripheral blood of C57BL/6J mice before and after G-CSF mobilization. And the expression of HIF-1α and osteocalcin (OCN) mRNA and protein were detected by RQ-PCR and immunohistochemistry. The number of osteoblasts in bone marrow specimens of mice was counted under the microscope.The proportion of LSK cells in peripheral blood began to increase at day 4 of G-CSF mobilization, and reached the peak at day 5, which was significantly higher than that of control group (P<0.05). There was no distinct difference in the expression of HIF-1α mRNA between bone marrow nucleated cells and osteoblasts of steady-state mice (P=0.073), while OCN mRNA was mainly expressed in osteoblasts, which was higher than that in bone marrow nucleated cells (P=0.034). After mobilization, the expression level of HIF-1α increased, but OCN decreased, and the number of endosteum osteoblasts decreased. The change of HIF-1α expression was later than that of OCN and was consistent with the proportion of LSK cells in peripheral blood.The expression of HIF-1α in bone marrow was increased during the mobilization of HSC mediated by G-CSF, and one of the mechanisms may be related to the peripheral migration of HSC induced by osteoblasts inhibition.低氧诱导因子1α在G-CSF诱导小鼠造血干细胞动员中的表达变化.探究小鼠骨髓微环境低氧诱导因子1α（HIF-1α）在G-CSF动员造血干细胞（HSC）过程中的表达变化及相关机制.采用流式细胞术检测注射G-CSF前后小鼠外周血Lin-Sca-1+ c-kit+（LSK）细胞比例，并采用RQ-PCR、免疫组化等方法检测G-CSF动员不同时间点小鼠骨髓及骨内膜HIF-1α、骨钙素（OCN）mRNA和蛋白的表达水平，光学显微镜下观察小鼠股骨标本成骨细胞数.G-CSF动员4 d 小鼠外周血LSK细胞比例开始升高，动员5 d 达到高峰，均较对照组显著升高（P<0.05）；稳态小鼠HIF-1α mRNA在骨髓有核细胞和骨内膜成骨细胞中表达差异无显著统计学意义（P=0.073），OCN mRNA主要表达于骨内膜细胞，显著高于骨髓有核细胞（P=0.034）；动员过程中HIF-1α基因、蛋白表达水平升高，OCN基因、蛋白表达水平下降，骨内膜成骨细胞数量减少(P<0.05)；HIF-1α的表达变化晚于OCN（P＝0.004），且与小鼠外周血LSK细胞比例变化趋势一致（P＝0.000）.在G-CSF介导的HSC动员过程中，骨髓HIF-1α表达增加，成骨细胞受抑引起HSC向外周迁移是导致其变化的原因之一." @default.
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- W4320032634 date "2023-02-01" @default.
- W4320032634 modified "2023-09-26" @default.
- W4320032634 title "[Expression Changes of Hypoxia-Inducible Factor-1α in G-CSF Induced Hematopoietic Stem Cell Mobilization]." @default.
- W4320032634 doi "https://doi.org/10.19746/j.cnki.issn.1009-2137.2023.01.035" @default.
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