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- W4320032843 abstract "Zinc finger nucleases (ZFNs) are programmable nucleases that have contributed significantly to past genome-editing research. They are now utilized much less owing to the advent of transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats and CRISPR-associated protein system (CRISPR-Cas). These new methods allow for easier generation of reagents that target genomic sequences of interest and are less labor-intensive than ZFNs at targeting desired sequences. However, fundamental ZFN patents have expired, enabling a wide range of their distribution for clinical and industrial applications. This article introduces a ZFN construction protocol that uses bacterial one-hybrid (B1H) selection and single-strand annealing (SSA) assay." @default.
- W4320032843 created "2023-02-12" @default.
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- W4320032843 date "2023-01-01" @default.
- W4320032843 modified "2023-10-14" @default.
- W4320032843 title "Construction and Evaluation of Zinc Finger Nucleases" @default.
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- W4320032843 doi "https://doi.org/10.1007/978-1-0716-3016-7_1" @default.
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