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- W4320032859 abstract "Advances in CRISPR/Cas9 genome editing technologies have allowed for the rapid generation of Cre-loxP conditional knockout mice. However, current strategies for genotyping flox mice, typically based on Sanger sequencing following cloning of target sequences from dozens of pups, are time-consuming. Here, we describe a rapid screening method for flox mice, using in vitro Cre recombination that can be performed using simple enzymatic reactions and enables detection of functional flox mouse within 1 day. In addition, we introduce an efficient strategy for subsequent sequence analysis by cloning of floxed regions using the In-Fusion system. Our genotyping pipeline reduces laborious tasks and thus contributes to the rapid selection of accurately edited flox mice." @default.
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- W4320032859 date "2023-01-01" @default.
- W4320032859 modified "2023-10-14" @default.
- W4320032859 title "Efficient Detection of Flox Mice Using In Vitro Cre Recombination" @default.
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- W4320032859 doi "https://doi.org/10.1007/978-1-0716-3016-7_12" @default.
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