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- W4320032959 abstract "Pigs are excellent large animal models owing to their several physiological and anatomical similarities to humans. Somatic cell nuclear transfer using gene-modified cells is the mainstream approach for generating genetically modified pigs. Recent advances in improving gene editors such as the CRISPR/Cas9 system have enabled direct gene modification in zygotes/embryos. Here, we describe the gene editing by electroporation of Cas9 protein (GEEP) method, an optimized electroporation-mediated method for the introduction of CRISPR/Cas9 into porcine zygotes/embryos. The simplicity and micromanipulation-free procedures are the major advantages of this method." @default.
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- W4320032959 date "2023-01-01" @default.
- W4320032959 modified "2023-10-14" @default.
- W4320032959 title "GEEP Method: An Optimized Electroporation-Mediated Gene Editing Approach for Establishment of Knockout Pig Lines" @default.
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- W4320032959 doi "https://doi.org/10.1007/978-1-0716-3016-7_22" @default.
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