Matches in SemOpenAlex for { <https://semopenalex.org/work/W4320033063> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W4320033063 endingPage "456" @default.
- W4320033063 startingPage "450" @default.
- W4320033063 abstract "Abstract Introduction Streptococcus agalactiae , also known as group B streptococci (GBS), is associated with invasive infections in neonates. Identification of GBS vaginal colonization in pregnant women before delivery is essential for treatment with antibiotics to prevent intrapartum vertical transmission to the newborn. This study was designed to evaluate applicability of two rapid real‐time PCRs in comparison to standard culture identification. Material and methods We compared the Xpert GBS assay, hereafter referred to as Xpert, and GenomEra GBS PCR, hereafter referred to as GenomEra. The standard culture identification consisted of two different agar plates as well as an enrichment broth. Results We analyzed vaginal samples of 260 pregnant women; 42 samples were tested GBS‐positive by using standard culture as a gold standard, 30 by Xpert, and 37 by GenomEra. Xpert and GenomEra assays performed with sensitivities of 71.4% and 88.1% as well as specificities of 98.6% and 99.1%, respectively. Twelve vaginal samples were false‐negative by Xpert and five samples by GenomEra. Interestingly, three negative Xpert results of standard culture‐positive samples exhibited high Ct‐values indicating the presence of GBS. If higher Ct‐values are taken into consideration, the sensitivity of Xpert increases up to 78.6%. Moreover, only three Xpert PCRs had to be repeated, whereas two Genomera were invalid even after repetition and further 15 GenomEra PCRs were repeated because of borderline results or inhibition of the PCR test. Conclusions In this study, GenomEra assay performed with a higher sensitivity than the Xpert PCR. On the other hand, the Xpert assay needs less hands‐on‐time for a sample preparation and requires approximately four‐fold less repetitions as compared to the GenomEra assay. This robust performance of the Xpert assay make it applicable as a rapid intrapartum point‐of‐care test, although a higher sensitivity would be desirable. Therefore, culture in the 35–37 week of gestation remains the gold standard to detect vaginal colonization." @default.
- W4320033063 created "2023-02-12" @default.
- W4320033063 creator A5018695509 @default.
- W4320033063 creator A5021706628 @default.
- W4320033063 creator A5046681755 @default.
- W4320033063 creator A5048881563 @default.
- W4320033063 creator A5069886485 @default.
- W4320033063 creator A5072841086 @default.
- W4320033063 creator A5084270164 @default.
- W4320033063 date "2023-02-11" @default.
- W4320033063 modified "2023-10-14" @default.
- W4320033063 title "Evaluation of two rapid commercial assays for detection of <i>Streptococcus agalactiae</i> from vaginal samples" @default.
- W4320033063 cites W1535359176 @default.
- W4320033063 cites W2031210272 @default.
- W4320033063 cites W2054599821 @default.
- W4320033063 cites W2089326901 @default.
- W4320033063 cites W2092329312 @default.
- W4320033063 cites W2119347566 @default.
- W4320033063 cites W2124354496 @default.
- W4320033063 cites W2168418588 @default.
- W4320033063 cites W2182119864 @default.
- W4320033063 cites W2765550429 @default.
- W4320033063 cites W2766178102 @default.
- W4320033063 cites W2767306302 @default.
- W4320033063 cites W2767555099 @default.
- W4320033063 cites W2906319657 @default.
- W4320033063 cites W2938989498 @default.
- W4320033063 cites W2998854015 @default.
- W4320033063 cites W3035554312 @default.
- W4320033063 cites W4235549447 @default.
- W4320033063 cites W4320033063 @default.
- W4320033063 doi "https://doi.org/10.1111/aogs.14519" @default.
- W4320033063 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36772902" @default.
- W4320033063 hasPublicationYear "2023" @default.
- W4320033063 type Work @default.
- W4320033063 citedByCount "2" @default.
- W4320033063 countsByYear W43200330632023 @default.
- W4320033063 crossrefType "journal-article" @default.
- W4320033063 hasAuthorship W4320033063A5018695509 @default.
- W4320033063 hasAuthorship W4320033063A5021706628 @default.
- W4320033063 hasAuthorship W4320033063A5046681755 @default.
- W4320033063 hasAuthorship W4320033063A5048881563 @default.
- W4320033063 hasAuthorship W4320033063A5069886485 @default.
- W4320033063 hasAuthorship W4320033063A5072841086 @default.
- W4320033063 hasAuthorship W4320033063A5084270164 @default.
- W4320033063 hasBestOaLocation W43200330631 @default.
- W4320033063 hasConcept C126322002 @default.
- W4320033063 hasConcept C2777860557 @default.
- W4320033063 hasConcept C2778276568 @default.
- W4320033063 hasConcept C2778660310 @default.
- W4320033063 hasConcept C40993552 @default.
- W4320033063 hasConcept C523546767 @default.
- W4320033063 hasConcept C54355233 @default.
- W4320033063 hasConcept C71924100 @default.
- W4320033063 hasConcept C86803240 @default.
- W4320033063 hasConcept C89423630 @default.
- W4320033063 hasConceptScore W4320033063C126322002 @default.
- W4320033063 hasConceptScore W4320033063C2777860557 @default.
- W4320033063 hasConceptScore W4320033063C2778276568 @default.
- W4320033063 hasConceptScore W4320033063C2778660310 @default.
- W4320033063 hasConceptScore W4320033063C40993552 @default.
- W4320033063 hasConceptScore W4320033063C523546767 @default.
- W4320033063 hasConceptScore W4320033063C54355233 @default.
- W4320033063 hasConceptScore W4320033063C71924100 @default.
- W4320033063 hasConceptScore W4320033063C86803240 @default.
- W4320033063 hasConceptScore W4320033063C89423630 @default.
- W4320033063 hasIssue "4" @default.
- W4320033063 hasLocation W43200330631 @default.
- W4320033063 hasLocation W43200330632 @default.
- W4320033063 hasLocation W43200330633 @default.
- W4320033063 hasOpenAccess W4320033063 @default.
- W4320033063 hasPrimaryLocation W43200330631 @default.
- W4320033063 hasRelatedWork W1774708834 @default.
- W4320033063 hasRelatedWork W2012251036 @default.
- W4320033063 hasRelatedWork W2042751357 @default.
- W4320033063 hasRelatedWork W2084807013 @default.
- W4320033063 hasRelatedWork W2098104901 @default.
- W4320033063 hasRelatedWork W2129428874 @default.
- W4320033063 hasRelatedWork W2140570433 @default.
- W4320033063 hasRelatedWork W2408943786 @default.
- W4320033063 hasRelatedWork W4313857701 @default.
- W4320033063 hasRelatedWork W3122606152 @default.
- W4320033063 hasVolume "102" @default.
- W4320033063 isParatext "false" @default.
- W4320033063 isRetracted "false" @default.
- W4320033063 workType "article" @default.