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- W4320038467 abstract "Peroxisomes are vital metabolic organelles whose matrix enzymes are imported from the cytosol in a folded state by the soluble receptor PEX5. The import mechanism has been challenging to decipher because of the lack of suitable in vitro systems. Here, we present a protocol for reconstituting matrix protein import using Xenopus egg extract. We describe how extract is prepared, how to replace endogenous PEX5 with recombinant versions, and how to perform and interpret a peroxisomal import reaction using a fluorescent cargo. For complete details on the use and execution of this protocol, please refer to Skowyra and Rapoport (2022).1." @default.
- W4320038467 created "2023-02-12" @default.
- W4320038467 creator A5063441963 @default.
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- W4320038467 date "2023-03-01" @default.
- W4320038467 modified "2023-09-30" @default.
- W4320038467 title "Cell-free reconstitution of peroxisomal matrix protein import using Xenopus egg extract" @default.
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- W4320038467 doi "https://doi.org/10.1016/j.xpro.2023.102111" @default.
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