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• W4320039991 abstract "ABSTRACT Bats (order Chiroptera ) are a major reservoir for emerging and re-emerging zoonotic viruses. Their tolerance towards highly pathogenic human viruses led to the hypothesis that bats may possess an especially active antiviral interferon (IFN) system. Here, we cloned and functionally characterized the virus RNA sensor, Retinoic Acid-Inducible Gene-I (RIG-I), from the “microbat” Myotis daubentonii (suborder Yangochiroptera ) and the “megabat” Rousettus aegyptiacus (suborder Yinpterochiroptera), and compared them to the human ortholog. Our data show that the overall sequence and domain organization is highly conserved and that all three RIG-I orthologs can mediate a similar IFN induction in response to viral RNA at 37° and 39°C, but not at 30°C. Like human RIG-I, bat RIG-Is were optimally activated by double stranded RNA containing a 5’-triphosphate end and required Mitochondrial Antiviral-Signalling Protein (MAVS) for antiviral signalling. Moreover, the RIG-I orthologs of humans and of R. aegyptiacus , but not of M. daubentonii , enable innate immune sensing of SARS-CoV-2 infection. Our results thus show that microbats and megabats express a RIG-I that is not substantially different from the human counterpart with respect to function, temperature dependency, antiviral signaling, and RNA ligand properties, and that human and megabat RIG-I are able to sense SARS-CoV-2 infection. IMPORTANCE A common hypothesis holds that bats (order Chiroptera ) are outstanding reservoirs for zoonotic viruses because of a special antiviral interferon (IFN) system. However, functional studies about key components of the bat IFN system are rare. RIG-I is a cellular sensor for viral RNA signatures that activates the antiviral signalling chain to induce IFN. We cloned and functionally characterized RIG-I genes from representatives of the suborders Yangochiroptera and Yinpterochiroptera . The bat RIG-Is were conserved in their sequence and domain organization, and similar to human RIG-I in (i) mediating virus- and IFN-activated gene expression, (ii) antiviral signalling, (iii) temperature dependence, and (iv) recognition of RNA ligands. Moreover, RIG-I of Rousettus aegyptiacus (suborder Yinpterochiroptera ) and of humans were found to recognize SARS-CoV-2 infection. Thus, members of both bat suborders encode RIG-Is that are comparable to their human counterpart. The ability of bats to harbour zoonotic viruses therefore seems due to other features." @default.
• W4320039991 created "2023-02-12" @default.
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• W4320039991 date "2023-02-11" @default.
• W4320039991 modified "2023-09-30" @default.
• W4320039991 title "Functional comparisons of the virus sensor RIG-I from humans, the microbat<i>Myotis daubentonii</i>, and the megabat<i>Rousettus aegyptiacus</i>, and their response to SARS-CoV-2 infection" @default.
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• W4320039991 cites W2018568861 @default.
• W4320039991 cites W2019874100 @default.
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• W4320039991 cites W2030170890 @default.
• W4320039991 cites W2033487527 @default.
• W4320039991 cites W2033864175 @default.
• W4320039991 cites W2042594196 @default.
• W4320039991 cites W2045931701 @default.
• W4320039991 cites W2047686996 @default.
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• W4320039991 cites W2054554285 @default.
• W4320039991 cites W2070333365 @default.
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• W4320039991 cites W2788713245 @default.
• W4320039991 cites W2799839529 @default.
• W4320039991 cites W2800741203 @default.
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• W4320039991 cites W2945934429 @default.
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• W4320039991 doi "https://doi.org/10.1101/2023.02.08.527785" @default.
• W4320039991 hasPublicationYear "2023" @default.
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