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- W4320162036 abstract "Nowadays, the quick detection of antibiotic resistance bacteria causes a major problem in the field of the development of new antibiotics against the resistant bacteria. To overcome this problem, genome editing tools like clustered regularly interspaced short palindromic repeats (CRISPR) can be used. The CRISPR-CAS system is useful for targeting and killing antibiotic-resistant bacteria by cleaving resistance genes. It is also used to detect antibioticresistant bacteria. CRISPR is made up of a single guide RNA and the CAS 9 protein. The single guide RNA is used to guide toward the target sequence, and the CAS 9 protein is an enzyme that cuts DNA and is used in conjunction with the guide RNA. This modified sgRNA contains a complementary sequence to that of the target resistance gene and recognizes the target resistance sequence; therefore, it is cleaved by CAS-9 protein, and the removal of the resistance gene turns bacteria into antibiotic-sensitive ones. One of the delivery systems of CRISPR into bacteria is via bacteriophage." @default.
- W4320162036 created "2023-02-13" @default.
- W4320162036 creator A5039681928 @default.
- W4320162036 date "2021-01-01" @default.
- W4320162036 modified "2023-09-27" @default.
- W4320162036 title "The Engineered CRISPR-CAS System is a Beneficial Biological Tool for Detecting and Combating Antibiotic Resistance Microbes" @default.
- W4320162036 doi "https://doi.org/10.54646/bijbnt.08" @default.
- W4320162036 hasPublicationYear "2021" @default.
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