FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4320480617> ?p ?o ?g. }

• W4320480617 abstract "Autophagy is a tightly regulated catabolic process involved in the degradation and recycling of proteins and organelles. Ubiquitination plays an important role in the regulation of autophagy. Vacuole Membrane Protein 1 (VMP1) is an essential autophagy protein. The expression of VMP1 in pancreatic cancer stem cells carrying the activated Kirsten rat sarcoma viral oncogene homolog (KRAS) triggers autophagy and enables therapy resistance. Using biochemical and cellular approaches, we identified ubiquitination as a posttranslational modification of VMP1 from the initial steps in autophagosome biogenesis. VMP1 remains ubiquitinated as part of the autophagosome membrane throughout autophagic flux until autolysosome formation. However, VMP1 is not degraded by autophagy, nor by the ubiquitin-proteasomal system. Mass spectrometry and immunoprecipitation showed that the cell division cycle protein cdt2 (Cdt2), the substrate recognition subunit of the E3 ligase complex associated with cancer, cullin-RING ubiquitin ligase complex 4 (CRL4), is a novel interactor of VMP1 and is involved in VMP1 ubiquitination. VMP1 ubiquitination decreases under the CRL inhibitor MLN4924 and increases with Cdt2 overexpression. Moreover, VMP1 recruitment and autophagosome formation is significantly affected by CRL inhibition. Our results indicate that ubiquitination is a novel post-translational modification of VMP1 during autophagy in human tumor cells. VMP1 ubiquitination may be of clinical relevance in tumor cell therapy resistance." @default.
• W4320480617 created "2023-02-14" @default.
• W4320480617 creator A5019428522 @default.
• W4320480617 creator A5023584721 @default.
• W4320480617 creator A5024573852 @default.
• W4320480617 creator A5025151121 @default.
• W4320480617 creator A5028459053 @default.
• W4320480617 creator A5043325325 @default.
• W4320480617 creator A5060712982 @default.
• W4320480617 creator A5065254160 @default.
• W4320480617 creator A5076437901 @default.
• W4320480617 creator A5078304405 @default.
• W4320480617 creator A5086854267 @default.
• W4320480617 date "2023-02-13" @default.
• W4320480617 modified "2023-10-06" @default.
• W4320480617 title "Ubiquitination is a novel post-translational modification of VMP1 in autophagy of human tumor cells" @default.
• W4320480617 cites W1964322868 @default.
• W4320480617 cites W1966110641 @default.
• W4320480617 cites W1967743942 @default.
• W4320480617 cites W1969587176 @default.
• W4320480617 cites W1972272556 @default.
• W4320480617 cites W1974702375 @default.
• W4320480617 cites W1975915706 @default.
• W4320480617 cites W1979339081 @default.
• W4320480617 cites W1999880229 @default.
• W4320480617 cites W2000111822 @default.
• W4320480617 cites W2001827754 @default.
• W4320480617 cites W2011290663 @default.
• W4320480617 cites W2020733843 @default.
• W4320480617 cites W2021753885 @default.
• W4320480617 cites W2031189137 @default.
• W4320480617 cites W2033683607 @default.
• W4320480617 cites W2051887481 @default.
• W4320480617 cites W2059637124 @default.
• W4320480617 cites W2066697766 @default.
• W4320480617 cites W2071519991 @default.
• W4320480617 cites W2074127682 @default.
• W4320480617 cites W2078013685 @default.
• W4320480617 cites W2079351077 @default.
• W4320480617 cites W2124644922 @default.
• W4320480617 cites W2125040419 @default.
• W4320480617 cites W2133284292 @default.
• W4320480617 cites W2166987747 @default.
• W4320480617 cites W2315925646 @default.
• W4320480617 cites W2420376897 @default.
• W4320480617 cites W2514327877 @default.
• W4320480617 cites W2617561678 @default.
• W4320480617 cites W2751363052 @default.
• W4320480617 cites W2754976080 @default.
• W4320480617 cites W2791691662 @default.
• W4320480617 cites W2896203757 @default.
• W4320480617 cites W2907859936 @default.
• W4320480617 cites W2909477364 @default.
• W4320480617 cites W2923237125 @default.
• W4320480617 cites W2969796003 @default.
• W4320480617 cites W2980453182 @default.
• W4320480617 cites W2995199710 @default.
• W4320480617 cites W3000522739 @default.
• W4320480617 cites W3005290316 @default.
• W4320480617 cites W3037970808 @default.
• W4320480617 cites W3091696541 @default.
• W4320480617 cites W3137875499 @default.
• W4320480617 cites W3154074861 @default.
• W4320480617 cites W319525891 @default.
• W4320480617 cites W4224243709 @default.
• W4320480617 doi "https://doi.org/10.1101/2023.02.13.528037" @default.
• W4320480617 hasPublicationYear "2023" @default.
• W4320480617 type Work @default.
• W4320480617 citedByCount "0" @default.
• W4320480617 crossrefType "posted-content" @default.
• W4320480617 hasAuthorship W4320480617A5019428522 @default.
• W4320480617 hasAuthorship W4320480617A5023584721 @default.
• W4320480617 hasAuthorship W4320480617A5024573852 @default.
• W4320480617 hasAuthorship W4320480617A5025151121 @default.
• W4320480617 hasAuthorship W4320480617A5028459053 @default.
• W4320480617 hasAuthorship W4320480617A5043325325 @default.
• W4320480617 hasAuthorship W4320480617A5060712982 @default.
• W4320480617 hasAuthorship W4320480617A5065254160 @default.
• W4320480617 hasAuthorship W4320480617A5076437901 @default.
• W4320480617 hasAuthorship W4320480617A5078304405 @default.
• W4320480617 hasAuthorship W4320480617A5086854267 @default.
• W4320480617 hasBestOaLocation W43204806171 @default.
• W4320480617 hasConcept C104317684 @default.
• W4320480617 hasConcept C134459356 @default.
• W4320480617 hasConcept C168296220 @default.
• W4320480617 hasConcept C185592680 @default.
• W4320480617 hasConcept C190283241 @default.
• W4320480617 hasConcept C203522944 @default.
• W4320480617 hasConcept C25602115 @default.
• W4320480617 hasConcept C2778567606 @default.
• W4320480617 hasConcept C55493867 @default.
• W4320480617 hasConcept C86803240 @default.
• W4320480617 hasConcept C95444343 @default.
• W4320480617 hasConceptScore W4320480617C104317684 @default.
• W4320480617 hasConceptScore W4320480617C134459356 @default.
• W4320480617 hasConceptScore W4320480617C168296220 @default.
• W4320480617 hasConceptScore W4320480617C185592680 @default.
• W4320480617 hasConceptScore W4320480617C190283241 @default.
• W4320480617 hasConceptScore W4320480617C203522944 @default.
• W4320480617 hasConceptScore W4320480617C25602115 @default.

• next