FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4320710511> ?p ?o ?g. }

• W4320710511 endingPage "498" @default.
• W4320710511 startingPage "482" @default.
• W4320710511 abstract "SUMMARY Polyamines such as spermidine and spermine are essential regulators of cell growth, differentiation, maintenance of ion balance and abiotic stress tolerance. Their levels are controlled by the spermidine/spermine N 1 ‐acetyltransferase (SSAT) via acetylation to promote either their degradation or export outside the cell as shown in mammals. Plant genomes contain at least one gene coding for SSAT (also named NATA for N ‐AcetylTransferase Activity). Combining kinetics, HPLC‐MS and crystallography, we show that three plant SSATs, one from the lower plant moss Physcomitrium patens and two from the higher plant Zea mays , acetylate various aliphatic polyamines and two amino acids lysine (Lys) and ornithine (Orn). Thus, plant SSATs exhibit a broad substrate specificity, unlike more specific human SSATs (hSSATs) as hSSAT1 targets polyamines, whereas hSSAT2 acetylates Lys and thiaLys. The crystal structures of two PpSSAT ternary complexes, one with Lys and CoA, the other with acetyl‐CoA and polyethylene glycol (mimicking spermine), reveal a different binding mode for polyamine versus amino acid substrates accompanied by structural rearrangements of both the coenzyme and the enzyme. Two arginine residues, unique among plant SSATs, hold the carboxyl group of amino acid substrates. The most abundant acetylated compound accumulated in moss was N 6 ‐acetyl‐Lys, whereas N 5 ‐acetyl‐Orn, known to be toxic for aphids, was found in maize. Both plant species contain very low levels of acetylated polyamines. The present study provides a detailed biochemical and structural basis of plant SSAT enzymes that can acetylate a wide range of substrates and likely play various roles in planta ." @default.
• W4320710511 created "2023-02-15" @default.
• W4320710511 creator A5002243493 @default.
• W4320710511 creator A5005912027 @default.
• W4320710511 creator A5023933652 @default.
• W4320710511 creator A5029943128 @default.
• W4320710511 creator A5041079702 @default.
• W4320710511 creator A5047064184 @default.
• W4320710511 creator A5053606564 @default.
• W4320710511 creator A5061028043 @default.
• W4320710511 creator A5070675783 @default.
• W4320710511 creator A5074102855 @default.
• W4320710511 creator A5076048751 @default.
• W4320710511 creator A5078846281 @default.
• W4320710511 creator A5080099250 @default.
• W4320710511 date "2023-03-16" @default.
• W4320710511 modified "2023-10-14" @default.
• W4320710511 title "Biochemical and structural basis of polyamine, lysine and ornithine acetylation catalyzed by spermine/spermidine <i>N</i>‐acetyl transferase in moss and maize" @default.
• W4320710511 cites W1603831700 @default.
• W4320710511 cites W1756802921 @default.
• W4320710511 cites W1816841556 @default.
• W4320710511 cites W1858041152 @default.
• W4320710511 cites W1876761025 @default.
• W4320710511 cites W1963957860 @default.
• W4320710511 cites W1965844660 @default.
• W4320710511 cites W1967154168 @default.
• W4320710511 cites W1974620743 @default.
• W4320710511 cites W1978543867 @default.
• W4320710511 cites W1983415168 @default.
• W4320710511 cites W1990401148 @default.
• W4320710511 cites W1999497038 @default.
• W4320710511 cites W2005157871 @default.
• W4320710511 cites W2009928589 @default.
• W4320710511 cites W2011483867 @default.
• W4320710511 cites W2014091897 @default.
• W4320710511 cites W2016765195 @default.
• W4320710511 cites W2017840815 @default.
• W4320710511 cites W2018685698 @default.
• W4320710511 cites W2042175709 @default.
• W4320710511 cites W2044092461 @default.
• W4320710511 cites W2053712835 @default.
• W4320710511 cites W2059631543 @default.
• W4320710511 cites W2061421971 @default.
• W4320710511 cites W2062876845 @default.
• W4320710511 cites W2068910828 @default.
• W4320710511 cites W2073841855 @default.
• W4320710511 cites W2090275302 @default.
• W4320710511 cites W2091589081 @default.
• W4320710511 cites W2098111625 @default.
• W4320710511 cites W2100465118 @default.
• W4320710511 cites W2103934714 @default.
• W4320710511 cites W2105554307 @default.
• W4320710511 cites W2108305160 @default.
• W4320710511 cites W2108684255 @default.
• W4320710511 cites W2108728523 @default.
• W4320710511 cites W2111211467 @default.
• W4320710511 cites W2112076524 @default.
• W4320710511 cites W2113815141 @default.
• W4320710511 cites W2120784944 @default.
• W4320710511 cites W2126928905 @default.
• W4320710511 cites W2131777290 @default.
• W4320710511 cites W2138217484 @default.
• W4320710511 cites W2141560122 @default.
• W4320710511 cites W2144081223 @default.
• W4320710511 cites W2145726343 @default.
• W4320710511 cites W2175026104 @default.
• W4320710511 cites W2236500561 @default.
• W4320710511 cites W2296763639 @default.
• W4320710511 cites W2909186883 @default.
• W4320710511 cites W2971627362 @default.
• W4320710511 cites W3217656085 @default.
• W4320710511 cites W4200362054 @default.
• W4320710511 cites W4220953490 @default.
• W4320710511 cites W4234232506 @default.
• W4320710511 cites W4248872320 @default.
• W4320710511 cites W4281790889 @default.
• W4320710511 doi "https://doi.org/10.1111/tpj.16148" @default.
• W4320710511 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36786691" @default.
• W4320710511 hasPublicationYear "2023" @default.
• W4320710511 type Work @default.
• W4320710511 citedByCount "1" @default.
• W4320710511 countsByYear W43207105112023 @default.
• W4320710511 crossrefType "journal-article" @default.
• W4320710511 hasAuthorship W4320710511A5002243493 @default.
• W4320710511 hasAuthorship W4320710511A5005912027 @default.
• W4320710511 hasAuthorship W4320710511A5023933652 @default.
• W4320710511 hasAuthorship W4320710511A5029943128 @default.
• W4320710511 hasAuthorship W4320710511A5041079702 @default.
• W4320710511 hasAuthorship W4320710511A5047064184 @default.
• W4320710511 hasAuthorship W4320710511A5053606564 @default.
• W4320710511 hasAuthorship W4320710511A5061028043 @default.
• W4320710511 hasAuthorship W4320710511A5070675783 @default.
• W4320710511 hasAuthorship W4320710511A5074102855 @default.
• W4320710511 hasAuthorship W4320710511A5076048751 @default.
• W4320710511 hasAuthorship W4320710511A5078846281 @default.
• W4320710511 hasAuthorship W4320710511A5080099250 @default.
• W4320710511 hasBestOaLocation W43207105111 @default.
• W4320710511 hasConcept C104317684 @default.

• next