FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4320711802> ?p ?o ?g. }

• W4320711802 abstract "In this study, we explore the relationship between community structure and transcriptional activity of ammonia-oxidizing bacteria during cold temperature nitrification failure in three parallel full-scale sequencing batch reactors (SBRs) treating municipal wastewater. In the three reactors, ammonia concentrations increased with declines in wastewater temperature below 15°C. We quantified and sequenced 16S rRNA and ammonia monooxygenase (amoA) gene fragments in DNA and RNA extracts from activated sludge samples collected from the SBRs during the warmer seasons (summer and fall) and when water temperatures were below 15°C (winter and spring). Taxonomic community composition of amoA genes and transcripts did not vary much between the warmer and colder seasons. However, we observed significant differences in amoA transcript copy numbers between fall (highest) and spring (lowest). Ammonia-oxidizing bacteria of the genus Nitrosomonas sp. could maintain their population abundance despite lowering their amoA gene expression during winter and spring. In spite of relatively low population abundance, an amoA amplicon sequence variant (ASV) cluster identified as most similar to the amoA gene of Nitrosospira briensis showed the highest amoA transcript-to-gene ratio throughout all four seasons, indicating that some nitrifiers remain active at wastewater temperatures below 15°C. Our results show that 16S rRNA and amoA gene copy numbers are limited predictors of cell activity. To optimize function and performance of mixed community bioprocesses, we need to collect high-resolution quantitative transcriptomic and potentially proteomic data to resolve the response of individual species to changes in environmental parameters in engineered systems. IMPORTANCE The diverse microbial community of activated sludge used in biological treatment systems exhibits dynamic seasonal shifts in community composition and activity. Many wastewater treatment plants in temperate/continental climates experience seasonal cold temperature nitrification failure. Seasonal nitrification failure is the discharge of elevated concentrations of ammonia (greater than 4 mg/liter) with treated wastewater during the winter (influent wastewater temperatures below 13°C). This study aims at expanding our understanding of how ammonia-oxidizing bacteria in activated sludge change in activity and growth across seasons. We quantified the ammonia monooxygenase (amoA) gene and transcript copy numbers using real-time PCR and sequenced the amoA amplicons to reveal community structure and activity changes of nitrifying microbial populations during seasonal nitrification failure in three full-scale sequencing batch reactors (SRBs) treating municipal wastewater. Relevant findings presented in this study contribute to explain seasonal nitrification performance variability in SRBs." @default.
• W4320711802 created "2023-02-15" @default.
• W4320711802 creator A5048797563 @default.
• W4320711802 creator A5075099400 @default.
• W4320711802 creator A5085281064 @default.
• W4320711802 date "2023-04-13" @default.
• W4320711802 modified "2023-10-01" @default.
• W4320711802 title "Ammonia-Oxidizing Bacteria Maintain Abundance but Lower <i>amoA</i> -Gene Expression during Cold Temperature Nitrification Failure in a Full-Scale Municipal Wastewater Treatment Plant" @default.
• W4320711802 cites W1534485236 @default.
• W4320711802 cites W1560702872 @default.
• W4320711802 cites W1596749601 @default.
• W4320711802 cites W1604029064 @default.
• W4320711802 cites W175804142 @default.
• W4320711802 cites W1878020421 @default.
• W4320711802 cites W1966931111 @default.
• W4320711802 cites W1984970137 @default.
• W4320711802 cites W1993231925 @default.
• W4320711802 cites W2000829688 @default.
• W4320711802 cites W2004091484 @default.
• W4320711802 cites W2014824557 @default.
• W4320711802 cites W2015639477 @default.
• W4320711802 cites W2020340700 @default.
• W4320711802 cites W2061956399 @default.
• W4320711802 cites W2062760400 @default.
• W4320711802 cites W2064309355 @default.
• W4320711802 cites W2072790968 @default.
• W4320711802 cites W2076159844 @default.
• W4320711802 cites W2078204715 @default.
• W4320711802 cites W2093317275 @default.
• W4320711802 cites W2094936941 @default.
• W4320711802 cites W2096344603 @default.
• W4320711802 cites W2098612608 @default.
• W4320711802 cites W2099249681 @default.
• W4320711802 cites W2099381655 @default.
• W4320711802 cites W2103296919 @default.
• W4320711802 cites W2104200271 @default.
• W4320711802 cites W2109281034 @default.
• W4320711802 cites W2110044203 @default.
• W4320711802 cites W2113878058 @default.
• W4320711802 cites W2114576684 @default.
• W4320711802 cites W2118478461 @default.
• W4320711802 cites W2119223686 @default.
• W4320711802 cites W2125454787 @default.
• W4320711802 cites W2125846972 @default.
• W4320711802 cites W2127166178 @default.
• W4320711802 cites W2130627820 @default.
• W4320711802 cites W2133940989 @default.
• W4320711802 cites W2145648693 @default.
• W4320711802 cites W2152797493 @default.
• W4320711802 cites W2160830727 @default.
• W4320711802 cites W2165259257 @default.
• W4320711802 cites W2165437036 @default.
• W4320711802 cites W2165533576 @default.
• W4320711802 cites W2165715909 @default.
• W4320711802 cites W2166655850 @default.
• W4320711802 cites W2176371198 @default.
• W4320711802 cites W2401404581 @default.
• W4320711802 cites W2409623764 @default.
• W4320711802 cites W2490378810 @default.
• W4320711802 cites W2537488842 @default.
• W4320711802 cites W2735557121 @default.
• W4320711802 cites W2747254098 @default.
• W4320711802 cites W2782607229 @default.
• W4320711802 cites W2784624716 @default.
• W4320711802 cites W2791709174 @default.
• W4320711802 cites W2793660486 @default.
• W4320711802 cites W2887136521 @default.
• W4320711802 cites W2917439049 @default.
• W4320711802 cites W2922079300 @default.
• W4320711802 cites W2943194904 @default.
• W4320711802 cites W2949450945 @default.
• W4320711802 cites W2967163687 @default.
• W4320711802 cites W2988593844 @default.
• W4320711802 cites W3010956524 @default.
• W4320711802 cites W3045248777 @default.
• W4320711802 cites W3146071034 @default.
• W4320711802 cites W4245354303 @default.
• W4320711802 cites W4252876644 @default.
• W4320711802 doi "https://doi.org/10.1128/spectrum.02571-22" @default.
• W4320711802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36786623" @default.
• W4320711802 hasPublicationYear "2023" @default.
• W4320711802 type Work @default.
• W4320711802 citedByCount "0" @default.
• W4320711802 crossrefType "journal-article" @default.
• W4320711802 hasAuthorship W4320711802A5048797563 @default.
• W4320711802 hasAuthorship W4320711802A5075099400 @default.
• W4320711802 hasAuthorship W4320711802A5085281064 @default.
• W4320711802 hasBestOaLocation W43207118021 @default.
• W4320711802 hasConcept C144024400 @default.
• W4320711802 hasConcept C149923435 @default.
• W4320711802 hasConcept C151730666 @default.
• W4320711802 hasConcept C164752452 @default.
• W4320711802 hasConcept C178790620 @default.
• W4320711802 hasConcept C185592680 @default.
• W4320711802 hasConcept C18903297 @default.
• W4320711802 hasConcept C2775960256 @default.
• W4320711802 hasConcept C2776179834 @default.
• W4320711802 hasConcept C2776384668 @default.
• W4320711802 hasConcept C2776650774 @default.
• W4320711802 hasConcept C2777730692 @default.

• next