FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4320857138> ?p ?o ?g. }

• W4320857138 endingPage "1688" @default.
• W4320857138 startingPage "1677" @default.
• W4320857138 abstract "The activation of cardiac fibroblasts (CFs) leads to overproduction of collagens and subsequently cardiac fibrosis. However, the regulatory mechanism of CF function in the process of cardiac fibrosis remains unclear. This work investigated the function of polypyrimidine tract binding protein 1 (PTBP1)/nuclear receptor NR4A1 (Nur77)/fatty acid-binding protein 5 (FABP5) axis in myocardial fibrosis.Cardiac fibrosis was induced in mice suffered left anterior descending ligation. In parallel, neonatal mouse CFs were isolated and stimulated with transforming growth factor-β1 (TGF-β1). Cardiac fibrosis was evaluated by Masson's trichrome staining. Expression of PTBP1, Nur77, FABP5, collagen I, and collagen III was measured by quantitative real-time PCR and western blotting. Proliferation of CFs was assessed by 5-ethynyl-2'-deoxyuridine assay. Molecular interaction was validated by RNA-binding protein immunoprecipitation, chromatin immunoprecipitation, and dual luciferase reporter assay. PTBP1 was up-regulated (P < 0.05), whereas Nur77 (P < 0.05) and FABP5 (P < 0.05) were down-regulated in the fibrotic hearts of mice and TGF-β1-exposed CFs. PTBP1 overexpression facilitated proliferation (P < 0.05) and collagen I (P < 0.05) and collagen III (P < 0.05) expression of CFs after stimulation with TGF-β1. PTBP1 reduced Nur77 stability (P < 0.05) to inhibit Nur77 expression (P < 0.05) in CFs. Nur77 bound to FABP5 promoter to promote the transcription (P < 0.05) and expression (P < 0.05) of FABP5. Silencing of Nur77 or FABP5 abolished the inhibitory effect of PTBP1 knockdown on proliferation (P < 0.05) and collagen I (P < 0.05) and collagen III (P < 0.05) expression of CFs in vitro. PTBP1 depletion ameliorated cardiac fibrosis (P < 0.05), α-smooth muscle actin (P < 0.05), and collagen I (P < 0.05) expression in myocardial infarction mice through regulating Nur77/FABP5 pathway (P < 0.05) in vivo.PTBP1 contributed to cardiac fibrosis via promoting CF proliferation and collagen deposition through Nur77 mRNA decay and subsequent transcription inhibition of FABP5. Our findings suggest that PTBP1/Nur77/FABP5 axis may be potential targets for cardiac fibrosis therapy." @default.
• W4320857138 created "2023-02-16" @default.
• W4320857138 creator A5002497057 @default.
• W4320857138 creator A5005390719 @default.
• W4320857138 creator A5007284633 @default.
• W4320857138 creator A5060188617 @default.
• W4320857138 creator A5062193442 @default.
• W4320857138 creator A5087631945 @default.
• W4320857138 creator A5090530318 @default.
• W4320857138 date "2023-02-14" @default.
• W4320857138 modified "2023-10-02" @default.
• W4320857138 title "Polypyrimidine tract binding protein 1 exacerbates cardiac fibrosis by regulating fatty acid‐binding protein 5" @default.
• W4320857138 cites W2033161660 @default.
• W4320857138 cites W2154455571 @default.
• W4320857138 cites W2176043226 @default.
• W4320857138 cites W2504477734 @default.
• W4320857138 cites W2798244438 @default.
• W4320857138 cites W2806290836 @default.
• W4320857138 cites W2806949879 @default.
• W4320857138 cites W2810619747 @default.
• W4320857138 cites W2883561022 @default.
• W4320857138 cites W2922469710 @default.
• W4320857138 cites W2988678805 @default.
• W4320857138 cites W3000815895 @default.
• W4320857138 cites W3004467613 @default.
• W4320857138 cites W3012909463 @default.
• W4320857138 cites W3015746061 @default.
• W4320857138 cites W3034543632 @default.
• W4320857138 cites W3039737946 @default.
• W4320857138 cites W3109907940 @default.
• W4320857138 cites W3110187527 @default.
• W4320857138 cites W3128312555 @default.
• W4320857138 cites W3159850138 @default.
• W4320857138 cites W3160942634 @default.
• W4320857138 cites W3173798202 @default.
• W4320857138 cites W3201982510 @default.
• W4320857138 cites W3212321641 @default.
• W4320857138 cites W3217343291 @default.
• W4320857138 cites W4205939716 @default.
• W4320857138 cites W4226148121 @default.
• W4320857138 cites W4320857138 @default.
• W4320857138 doi "https://doi.org/10.1002/ehf2.14318" @default.
• W4320857138 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36788730" @default.
• W4320857138 hasPublicationYear "2023" @default.
• W4320857138 type Work @default.
• W4320857138 citedByCount "2" @default.
• W4320857138 countsByYear W43208571382023 @default.
• W4320857138 crossrefType "journal-article" @default.
• W4320857138 hasAuthorship W4320857138A5002497057 @default.
• W4320857138 hasAuthorship W4320857138A5005390719 @default.
• W4320857138 hasAuthorship W4320857138A5007284633 @default.
• W4320857138 hasAuthorship W4320857138A5060188617 @default.
• W4320857138 hasAuthorship W4320857138A5062193442 @default.
• W4320857138 hasAuthorship W4320857138A5087631945 @default.
• W4320857138 hasAuthorship W4320857138A5090530318 @default.
• W4320857138 hasBestOaLocation W43208571381 @default.
• W4320857138 hasConcept C104317684 @default.
• W4320857138 hasConcept C105580179 @default.
• W4320857138 hasConcept C126322002 @default.
• W4320857138 hasConcept C134018914 @default.
• W4320857138 hasConcept C153911025 @default.
• W4320857138 hasConcept C173396325 @default.
• W4320857138 hasConcept C185592680 @default.
• W4320857138 hasConcept C190283241 @default.
• W4320857138 hasConcept C2777420927 @default.
• W4320857138 hasConcept C2777793812 @default.
• W4320857138 hasConcept C2780559512 @default.
• W4320857138 hasConcept C41282012 @default.
• W4320857138 hasConcept C502942594 @default.
• W4320857138 hasConcept C55493867 @default.
• W4320857138 hasConcept C71924100 @default.
• W4320857138 hasConcept C86803240 @default.
• W4320857138 hasConceptScore W4320857138C104317684 @default.
• W4320857138 hasConceptScore W4320857138C105580179 @default.
• W4320857138 hasConceptScore W4320857138C126322002 @default.
• W4320857138 hasConceptScore W4320857138C134018914 @default.
• W4320857138 hasConceptScore W4320857138C153911025 @default.
• W4320857138 hasConceptScore W4320857138C173396325 @default.
• W4320857138 hasConceptScore W4320857138C185592680 @default.
• W4320857138 hasConceptScore W4320857138C190283241 @default.
• W4320857138 hasConceptScore W4320857138C2777420927 @default.
• W4320857138 hasConceptScore W4320857138C2777793812 @default.
• W4320857138 hasConceptScore W4320857138C2780559512 @default.
• W4320857138 hasConceptScore W4320857138C41282012 @default.
• W4320857138 hasConceptScore W4320857138C502942594 @default.
• W4320857138 hasConceptScore W4320857138C55493867 @default.
• W4320857138 hasConceptScore W4320857138C71924100 @default.
• W4320857138 hasConceptScore W4320857138C86803240 @default.
• W4320857138 hasIssue "3" @default.
• W4320857138 hasLocation W43208571381 @default.
• W4320857138 hasLocation W43208571382 @default.
• W4320857138 hasLocation W43208571383 @default.
• W4320857138 hasOpenAccess W4320857138 @default.
• W4320857138 hasPrimaryLocation W43208571381 @default.
• W4320857138 hasRelatedWork W1897701706 @default.
• W4320857138 hasRelatedWork W2032029448 @default.
• W4320857138 hasRelatedWork W2748952813 @default.
• W4320857138 hasRelatedWork W2779354261 @default.

• next