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- W4320914366 abstract "<ns4:p><ns4:bold>Background:</ns4:bold> Fusarium rot is a newly introduced, devastating disease of citrus fruits. The current investigation was undertaken to characterize the microbes responsible for fruit rot in <ns4:italic>Citrus reticulata</ns4:italic>.</ns4:p><ns4:p> <ns4:bold>Methods:</ns4:bold> Pathogens were isolated from infected citrus fruits using morphological and molecular approaches. For confirmation of the isolated fungi, polymerase chain reaction (PCR) amplification and internal transcribed spacer gene sequencing techniques were used.</ns4:p><ns4:p> <ns4:bold>Results:</ns4:bold> The isolated fungus was grown on potato dextrose agar for three days and it produced clamydospores, hyphae and macroconidia. PCR amplification of isolated fungal DNA gave a 650 bp product. The sequence obtained from isolated fungi had 99.42% similarity with the reference <ns4:italic>Fusarium concentricum</ns4:italic> sequence in NCBI GenBank. The obtained sequence was deposited in GenBank (Accession No. <ns4:ext-link xmlns:ns5=http://www.w3.org/1999/xlink ext-link-type=uri ns5:href=https://www.ncbi.nlm.nih.gov/nuccore/MT856371>MT856371</ns4:ext-link>). Two isolates showed virulence capability on fresh guava, sweet orange and tomato fruits, which confirmed species identification and Koch’s postulates. Artificially inoculated fungal species grown on tested fruits showed typical <ns4:italic>Fusarium</ns4:italic> species symptoms.</ns4:p><ns4:p> <ns4:bold>Conclusions:</ns4:bold> Outcomes of the present study are beneficial for the detection of this detrimental disease in postharvest <ns4:italic>Citrus reticulata </ns4:italic>fruits. Further research is needed for the control of this economically important disease. This is the first study of fruit rot in <ns4:italic>Citrus reticulata </ns4:italic>caused by <ns4:ext-link xmlns:ns5=http://www.w3.org/1999/xlink ext-link-type=uri ns5:href=https://en.wikipedia.org/wiki/Fusarium><ns4:italic>Fusarium</ns4:italic></ns4:ext-link> in Bangladesh.</ns4:p>" @default.
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- W4320914366 date "2023-02-15" @default.
- W4320914366 modified "2023-09-24" @default.
- W4320914366 title "First report on molecular identification of Fusarium species causing fruit rot of mandarin (Citrus reticulata) in Bangladesh" @default.
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