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- W4321265089 abstract "The expectation-maximization (EM) algorithm based on maximum-likelihood estimation (MLE) has been used for restoring simulated quantum-limited noncoherent imagery in our previous work. 1 Its performance in these computer simulations has motivated us to further test the algorithm on three-dimensional (3-D) real data. In recent experiments, we used an accelerative version of the EM algorithm for this testing. 2 3-D optically sectioned images of a fluorescently labeled biological specimen were used in these experiments. These 3-D real data were collected as a stack of 32 128 × 128 images. To reduce the computer execution time and memory space, instead of reconstructing the full 3-D image, we reconstructed a pair of stereo images by applying the slice theorem of computerized tomography (CT). Fig. 1 shows the 17th slice out of the stack of the 32 collected images. This image contains both in focus and out of focus portions. A stereo pair reconstructed by MLE after 100 iterations is shown in Fig. 2." @default.
- W4321265089 created "2023-02-18" @default.
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- W4321265089 date "1990-01-01" @default.
- W4321265089 modified "2023-09-23" @default.
- W4321265089 title "Stereo images of fluorescence micrographs through maximum-likelihood estimation" @default.
- W4321265089 cites W2091244702 @default.
- W4321265089 doi "https://doi.org/10.1364/oam.1990.tuoo3" @default.
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