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- W4321381307 endingPage "101783" @default.
- W4321381307 startingPage "101783" @default.
- W4321381307 abstract "There is a need for rapid and field-based bacterial detection and identification platforms that do not rely on lengthy growth cultures and lab-based techniques such as mass spectroscopy and polymerase chain reaction. Electrochemical techniques have been developed for culture-free bacterial detection; however, biosensors that deliver a low enough limit-of-detection for direct water pathogen sensing remain elusive. Herein, we report a photoelectrochemical biosensor using photoactive DNAzymes for bacterial identification and photoelectrochemical signal transduction. The signal transduction is based on using DNA as a switch that makes and breaks semiconductor heterostructures between TiO2 nanomaterials for target induced-signal modulation. The developed assay, integrating molecular and photoelectrochemical switching, is able to detect Escherichia coli at a concentration of 18 CFU mL−1 and quantify it over five orders-of-magnitude in lake water without target enrichment, addition of reagents, or sample processing and can specifically identify this organism amongst five similar bacterial pathogens." @default.
- W4321381307 created "2023-02-21" @default.
- W4321381307 creator A5000593583 @default.
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- W4321381307 creator A5088949896 @default.
- W4321381307 date "2023-04-01" @default.
- W4321381307 modified "2023-10-13" @default.
- W4321381307 title "Programming synthetic DNA molecules for building and breaking heterostructures between TiO2 nanomaterials for photoelectrochemical bacterial detection" @default.
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