Matches in SemOpenAlex for { <https://semopenalex.org/work/W4321429523> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W4321429523 endingPage "8140" @default.
- W4321429523 startingPage "8125" @default.
- W4321429523 abstract "BACKGROUND:Gastroenteropancreatic neuroendocrine tumors (GEP-NETs) has had a significant increase over the past 4 decades. The pathophysiological role of the cyclooxygenase-2 (cox-2) gene and factors responsible for the expression in GEP-NETs is of clinical value. Current study determined the expression of cox-2 gene in human GEP-NET tissues and corresponding cell lines, investigated the molecular mechanisms underlying the regulation of cox-2 gene expression and assessed the effect of nonsteroidal anti-inflammatory drugs (NSAIDs) on both anchorage-dependent and independent growth of GEP-NET cells. MATERIAL AND METHODS:GEP-NET tissues and QGP-1, BON, and LCC-18 GEP-NET cell lines were used. The expression of cox-2 gene was analyzed by immunohistochemistry, western blot, RT-PCR, and enzyme immunoassay. Transient transfection and luciferase assays along with electrophoretic mobility shift assays were conducted to explore the regulation of cox-2 gene expression. The effect of COX-inhibitors on GEP-NET cell growth was determined by proliferation assays and colony growth assessment. RESULTS:We found 87.8% of GEP-NET tissues stained positive for COX-2. QGP-1 and LCC-18 cells expressed cox-2 gene. PGE2 (prostaglandin E2) amounts quantified in the supernatants of NET cells matched to cox-2 expression level. The CRE-E-box element (–56 to –48 bp) and binding of USF1, USF2, and CREB transcription factors to this proximal promoter element were essential for cox-2 promoter activity in GEP-NET cells. COX-2-specific inhibitor NS-398 potently and dose-dependently inhibited PGE2 release from QGP-1 cells. Interestingly, both NS-398 and acetylic salicylic acid effectively suppressed proliferation of QGP-1 and BON cells in a dose-dependent manner. CONCLUSIONS:The majority of GEP-NETs over express cox-2 gene. The binding of CREB and USF-1/-2 transcription factors to a proximal, overlapping CRE-Ebox element is the underlying mechanism for cox-2 gene expression. NSAIDs potently suppressed the proliferations and may offer a novel approach for chemoprevention and therapy of GEP-NETs." @default.
- W4321429523 created "2023-02-22" @default.
- W4321429523 creator A5012763680 @default.
- W4321429523 creator A5015723785 @default.
- W4321429523 creator A5021174030 @default.
- W4321429523 creator A5081534603 @default.
- W4321429523 creator A5086953588 @default.
- W4321429523 date "2018-11-13" @default.
- W4321429523 modified "2023-10-09" @default.
- W4321429523 title "Expression and Molecular Regulation of the Cox2 Gene in Gastroenteropancreatic Neuroendocrine Tumors and Antiproliferation of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs)" @default.
- W4321429523 doi "https://doi.org/10.12659/msm.912419" @default.
- W4321429523 hasPublicationYear "2018" @default.
- W4321429523 type Work @default.
- W4321429523 citedByCount "6" @default.
- W4321429523 countsByYear W43214295232019 @default.
- W4321429523 countsByYear W43214295232020 @default.
- W4321429523 countsByYear W43214295232021 @default.
- W4321429523 countsByYear W43214295232023 @default.
- W4321429523 crossrefType "journal-article" @default.
- W4321429523 hasAuthorship W4321429523A5012763680 @default.
- W4321429523 hasAuthorship W4321429523A5015723785 @default.
- W4321429523 hasAuthorship W4321429523A5021174030 @default.
- W4321429523 hasAuthorship W4321429523A5081534603 @default.
- W4321429523 hasAuthorship W4321429523A5086953588 @default.
- W4321429523 hasBestOaLocation W43214295232 @default.
- W4321429523 hasConcept C104317684 @default.
- W4321429523 hasConcept C134018914 @default.
- W4321429523 hasConcept C150194340 @default.
- W4321429523 hasConcept C153911025 @default.
- W4321429523 hasConcept C2776415932 @default.
- W4321429523 hasConcept C2777956040 @default.
- W4321429523 hasConcept C502942594 @default.
- W4321429523 hasConcept C54009773 @default.
- W4321429523 hasConcept C54355233 @default.
- W4321429523 hasConcept C55493867 @default.
- W4321429523 hasConcept C81885089 @default.
- W4321429523 hasConcept C86803240 @default.
- W4321429523 hasConceptScore W4321429523C104317684 @default.
- W4321429523 hasConceptScore W4321429523C134018914 @default.
- W4321429523 hasConceptScore W4321429523C150194340 @default.
- W4321429523 hasConceptScore W4321429523C153911025 @default.
- W4321429523 hasConceptScore W4321429523C2776415932 @default.
- W4321429523 hasConceptScore W4321429523C2777956040 @default.
- W4321429523 hasConceptScore W4321429523C502942594 @default.
- W4321429523 hasConceptScore W4321429523C54009773 @default.
- W4321429523 hasConceptScore W4321429523C54355233 @default.
- W4321429523 hasConceptScore W4321429523C55493867 @default.
- W4321429523 hasConceptScore W4321429523C81885089 @default.
- W4321429523 hasConceptScore W4321429523C86803240 @default.
- W4321429523 hasLocation W43214295231 @default.
- W4321429523 hasLocation W43214295232 @default.
- W4321429523 hasLocation W43214295233 @default.
- W4321429523 hasOpenAccess W4321429523 @default.
- W4321429523 hasPrimaryLocation W43214295231 @default.
- W4321429523 hasRelatedWork W1980609410 @default.
- W4321429523 hasRelatedWork W2285828597 @default.
- W4321429523 hasRelatedWork W2361321344 @default.
- W4321429523 hasRelatedWork W2372521113 @default.
- W4321429523 hasRelatedWork W2390799392 @default.
- W4321429523 hasRelatedWork W2391256396 @default.
- W4321429523 hasRelatedWork W2392804297 @default.
- W4321429523 hasRelatedWork W2469005932 @default.
- W4321429523 hasRelatedWork W2978251436 @default.
- W4321429523 hasRelatedWork W3157865522 @default.
- W4321429523 hasVolume "24" @default.
- W4321429523 isParatext "false" @default.
- W4321429523 isRetracted "false" @default.
- W4321429523 workType "article" @default.