FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4321440668> ?p ?o ?g. }

• W4321440668 abstract "The enediynes are structurally characterized by a 1,5-diyne-3-ene motif within a 9- or 10-membered enediyne core. The anthraquinone-fused enediynes (AFEs) are a subclass of 10-membered enediynes that contain an anthraquinone moiety fused to the enediyne core as exemplified by dynemicins and tiancimycins. A conserved iterative type I polyketide synthase (PKSE) is known to initiate the biosynthesis of all enediyne cores, and evidence has recently been reported to suggest that the anthraquinone moiety also originates from the PKSE product. However, the identity of the PKSE product that is converted to the enediyne core or anthraquinone moiety has not been established. Here, we report the utilization of recombinant E. coli coexpressing various combinations of genes that encode a PKSE and a thioesterase (TE) from either 9- or 10-membered enediyne biosynthetic gene clusters to chemically complement ΔPKSE mutant strains of the producers of dynemicins and tiancimycins. Additionally, 13C-labeling experiments were performed to track the fate of the PKSE/TE product in the ΔPKSE mutants. These studies reveal that 1,3,5,7,9,11,13-pentadecaheptaene is the nascent, discrete product of the PKSE/TE that is converted to the enediyne core. Furthermore, a second molecule of 1,3,5,7,9,11,13-pentadecaheptaene is demonstrated to serve as the precursor of the anthraquinone moiety. The results establish a unified biosynthetic paradigm for AFEs, solidify an unprecedented biosynthetic logic for aromatic polyketides, and have implications for the biosynthesis of not only AFEs but all enediynes." @default.
• W4321440668 created "2023-02-22" @default.
• W4321440668 creator A5003353735 @default.
• W4321440668 creator A5009635317 @default.
• W4321440668 creator A5018889599 @default.
• W4321440668 creator A5024198719 @default.
• W4321440668 creator A5039964857 @default.
• W4321440668 creator A5043661399 @default.
• W4321440668 creator A5049187852 @default.
• W4321440668 creator A5056674771 @default.
• W4321440668 creator A5066162156 @default.
• W4321440668 creator A5066390705 @default.
• W4321440668 creator A5077557326 @default.
• W4321440668 creator A5087514362 @default.
• W4321440668 date "2023-02-21" @default.
• W4321440668 modified "2023-10-16" @default.
• W4321440668 title "A discrete intermediate for the biosynthesis of both the enediyne core and the anthraquinone moiety of enediyne natural products" @default.
• W4321440668 cites W125268406 @default.
• W4321440668 cites W1653566113 @default.
• W4321440668 cites W1933115513 @default.
• W4321440668 cites W1965209748 @default.
• W4321440668 cites W1970178125 @default.
• W4321440668 cites W1983943598 @default.
• W4321440668 cites W1985408763 @default.
• W4321440668 cites W1985917132 @default.
• W4321440668 cites W1990163407 @default.
• W4321440668 cites W1993886550 @default.
• W4321440668 cites W1995886274 @default.
• W4321440668 cites W1999546902 @default.
• W4321440668 cites W2002711269 @default.
• W4321440668 cites W2009045748 @default.
• W4321440668 cites W2022103709 @default.
• W4321440668 cites W2027069759 @default.
• W4321440668 cites W2036429461 @default.
• W4321440668 cites W2036561818 @default.
• W4321440668 cites W2038979178 @default.
• W4321440668 cites W2070363332 @default.
• W4321440668 cites W2071446702 @default.
• W4321440668 cites W2082529739 @default.
• W4321440668 cites W2088808945 @default.
• W4321440668 cites W2093969225 @default.
• W4321440668 cites W2098442675 @default.
• W4321440668 cites W2132536746 @default.
• W4321440668 cites W2134225148 @default.
• W4321440668 cites W2149779144 @default.
• W4321440668 cites W2153554955 @default.
• W4321440668 cites W2188824440 @default.
• W4321440668 cites W2321620272 @default.
• W4321440668 cites W2561858807 @default.
• W4321440668 cites W2751910324 @default.
• W4321440668 cites W2769611774 @default.
• W4321440668 cites W2791077649 @default.
• W4321440668 cites W2889713599 @default.
• W4321440668 cites W2949332250 @default.
• W4321440668 cites W2955715715 @default.
• W4321440668 cites W2970130444 @default.
• W4321440668 cites W3000681910 @default.
• W4321440668 cites W3004895557 @default.
• W4321440668 cites W3011719546 @default.
• W4321440668 cites W3127486021 @default.
• W4321440668 cites W3173021105 @default.
• W4321440668 cites W3183816839 @default.
• W4321440668 cites W3207641289 @default.
• W4321440668 cites W4294124094 @default.
• W4321440668 cites W4307229268 @default.
• W4321440668 cites W4311281541 @default.
• W4321440668 doi "https://doi.org/10.1073/pnas.2220468120" @default.
• W4321440668 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36802426" @default.
• W4321440668 hasPublicationYear "2023" @default.
• W4321440668 type Work @default.
• W4321440668 citedByCount "3" @default.
• W4321440668 countsByYear W43214406682023 @default.
• W4321440668 crossrefType "journal-article" @default.
• W4321440668 hasAuthorship W4321440668A5003353735 @default.
• W4321440668 hasAuthorship W4321440668A5009635317 @default.
• W4321440668 hasAuthorship W4321440668A5018889599 @default.
• W4321440668 hasAuthorship W4321440668A5024198719 @default.
• W4321440668 hasAuthorship W4321440668A5039964857 @default.
• W4321440668 hasAuthorship W4321440668A5043661399 @default.
• W4321440668 hasAuthorship W4321440668A5049187852 @default.
• W4321440668 hasAuthorship W4321440668A5056674771 @default.
• W4321440668 hasAuthorship W4321440668A5066162156 @default.
• W4321440668 hasAuthorship W4321440668A5066390705 @default.
• W4321440668 hasAuthorship W4321440668A5077557326 @default.
• W4321440668 hasAuthorship W4321440668A5087514362 @default.
• W4321440668 hasBestOaLocation W43214406681 @default.
• W4321440668 hasConcept C104317684 @default.
• W4321440668 hasConcept C178790620 @default.
• W4321440668 hasConcept C185592680 @default.
• W4321440668 hasConcept C2776568683 @default.
• W4321440668 hasConcept C2777566158 @default.
• W4321440668 hasConcept C2777763344 @default.
• W4321440668 hasConcept C2778635478 @default.
• W4321440668 hasConcept C2778913731 @default.
• W4321440668 hasConcept C2779396153 @default.
• W4321440668 hasConcept C553450214 @default.
• W4321440668 hasConcept C55493867 @default.
• W4321440668 hasConcept C71240020 @default.
• W4321440668 hasConceptScore W4321440668C104317684 @default.
• W4321440668 hasConceptScore W4321440668C178790620 @default.

• next