FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4321443933> ?p ?o ?g. }

• W4321443933 abstract "Abstract Background Multiple myeloma (MM) is a ubiquitin proteasome system (UPS)-dysfunction disease. We previously reported that the PRAME transcript level at diagnosis was prognostic for MM, which was related to proteasome inhibitor bortezomib treatment. In the present study, we aimed to investigate molecular mechanisms underlying the above clinical performance in MM cells. Methods MM cell lines with PRAME knockdown and overexpression were established by lentivirus transduction. Cell viability, cell cycle analysis, immunohistochemistry staining, cell migration and invasion, colony-forming and xeno-transplant assays were performed to evaluate the biological effects of PRAME on MM cells in vivo and in vitro. Proteomics and IP combined with MS were further performed to explore the downstream signaling. Co-IP, western blot, cycloheximide (CHX)-chase assay, and endogenous ubiquitination assay were utilized to examine the interactions and ubiquitination relations between PRAME and CTMP as well as p21. Assessment of apoptosis and CHX-chase assay were applied to analyze the role of PRAME under the effect of bortezomib on MM cells. Results Proliferation-promoting role of PRAME was demonstrated in MM cell models. CTMP and p21 were found to be the novel targets of PRAME in the Cul2-dependent substrate recognition process. PRAME interacted with and mediated ubiquitination of CTMP and p21, and subsequently elevated p-Akt and CCND3 protein levels, and promoted apoptosis of MM cells under bortezomib treatment. Conclusions PRAME promoted proliferation and increased bortezomib sensibility by regulating ubiquitination and degradation of CTMP and p21, which provided new targets for more precise and effective treatment choices for MM." @default.
• W4321443933 created "2023-02-22" @default.
• W4321443933 creator A5004323293 @default.
• W4321443933 creator A5017100408 @default.
• W4321443933 creator A5019415924 @default.
• W4321443933 creator A5033746402 @default.
• W4321443933 creator A5037009651 @default.
• W4321443933 creator A5043221610 @default.
• W4321443933 creator A5051363455 @default.
• W4321443933 creator A5060002817 @default.
• W4321443933 creator A5064813104 @default.
• W4321443933 date "2023-02-21" @default.
• W4321443933 modified "2023-10-04" @default.
• W4321443933 title "PRAME promotes proliferation of multiple myeloma cells through CTMP/Akt/p21/CCND3 axis by regulating the ubiquitination of CTMP and p21" @default.
• W4321443933 cites W1967838516 @default.
• W4321443933 cites W1992692251 @default.
• W4321443933 cites W1993191461 @default.
• W4321443933 cites W2015456709 @default.
• W4321443933 cites W2041258148 @default.
• W4321443933 cites W2060311551 @default.
• W4321443933 cites W2067653691 @default.
• W4321443933 cites W2073241357 @default.
• W4321443933 cites W2075632851 @default.
• W4321443933 cites W2101177010 @default.
• W4321443933 cites W2111423083 @default.
• W4321443933 cites W2118587431 @default.
• W4321443933 cites W2125685908 @default.
• W4321443933 cites W2140510830 @default.
• W4321443933 cites W2143979757 @default.
• W4321443933 cites W2144408457 @default.
• W4321443933 cites W2290463785 @default.
• W4321443933 cites W2346138661 @default.
• W4321443933 cites W2472119149 @default.
• W4321443933 cites W2759087703 @default.
• W4321443933 cites W2773382289 @default.
• W4321443933 cites W2923065903 @default.
• W4321443933 cites W2928665623 @default.
• W4321443933 cites W2941605147 @default.
• W4321443933 cites W3046528269 @default.
• W4321443933 cites W3113055450 @default.
• W4321443933 cites W3121778797 @default.
• W4321443933 cites W3134563687 @default.
• W4321443933 cites W4210734847 @default.
• W4321443933 cites W4211058750 @default.
• W4321443933 cites W4285148186 @default.
• W4321443933 cites W4294051601 @default.
• W4321443933 doi "https://doi.org/10.21203/rs.3.rs-2602154/v1" @default.
• W4321443933 hasPublicationYear "2023" @default.
• W4321443933 type Work @default.
• W4321443933 citedByCount "0" @default.
• W4321443933 crossrefType "posted-content" @default.
• W4321443933 hasAuthorship W4321443933A5004323293 @default.
• W4321443933 hasAuthorship W4321443933A5017100408 @default.
• W4321443933 hasAuthorship W4321443933A5019415924 @default.
• W4321443933 hasAuthorship W4321443933A5033746402 @default.
• W4321443933 hasAuthorship W4321443933A5037009651 @default.
• W4321443933 hasAuthorship W4321443933A5043221610 @default.
• W4321443933 hasAuthorship W4321443933A5051363455 @default.
• W4321443933 hasAuthorship W4321443933A5060002817 @default.
• W4321443933 hasAuthorship W4321443933A5064813104 @default.
• W4321443933 hasBestOaLocation W43214439331 @default.
• W4321443933 hasConcept C104317684 @default.
• W4321443933 hasConcept C185592680 @default.
• W4321443933 hasConcept C190283241 @default.
• W4321443933 hasConcept C203014093 @default.
• W4321443933 hasConcept C25602115 @default.
• W4321443933 hasConcept C27740335 @default.
• W4321443933 hasConcept C2776364478 @default.
• W4321443933 hasConcept C2777478702 @default.
• W4321443933 hasConcept C2778367456 @default.
• W4321443933 hasConcept C502942594 @default.
• W4321443933 hasConcept C53227056 @default.
• W4321443933 hasConcept C55493867 @default.
• W4321443933 hasConcept C62112901 @default.
• W4321443933 hasConcept C75217442 @default.
• W4321443933 hasConcept C86803240 @default.
• W4321443933 hasConcept C95444343 @default.
• W4321443933 hasConceptScore W4321443933C104317684 @default.
• W4321443933 hasConceptScore W4321443933C185592680 @default.
• W4321443933 hasConceptScore W4321443933C190283241 @default.
• W4321443933 hasConceptScore W4321443933C203014093 @default.
• W4321443933 hasConceptScore W4321443933C25602115 @default.
• W4321443933 hasConceptScore W4321443933C27740335 @default.
• W4321443933 hasConceptScore W4321443933C2776364478 @default.
• W4321443933 hasConceptScore W4321443933C2777478702 @default.
• W4321443933 hasConceptScore W4321443933C2778367456 @default.
• W4321443933 hasConceptScore W4321443933C502942594 @default.
• W4321443933 hasConceptScore W4321443933C53227056 @default.
• W4321443933 hasConceptScore W4321443933C55493867 @default.
• W4321443933 hasConceptScore W4321443933C62112901 @default.
• W4321443933 hasConceptScore W4321443933C75217442 @default.
• W4321443933 hasConceptScore W4321443933C86803240 @default.
• W4321443933 hasConceptScore W4321443933C95444343 @default.
• W4321443933 hasLocation W43214439331 @default.
• W4321443933 hasOpenAccess W4321443933 @default.
• W4321443933 hasPrimaryLocation W43214439331 @default.
• W4321443933 hasRelatedWork W2063424779 @default.
• W4321443933 hasRelatedWork W2215693941 @default.
• W4321443933 hasRelatedWork W2321752808 @default.
• W4321443933 hasRelatedWork W2353555820 @default.

• next