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• W4321454507 abstract "The CGA codon is a rare codon in Saccharomyces cerevisiae and is known to be inefficiently decoded by wobble pairing with Arg‐tRNA(ICG). The tRNA Arg (ICG) is post‐transcriptionally edited from tRNA Arg (ACG) by the anticodon first adenosine deamination enzyme Tad2/Tad3 complex. Experimental consecutive CGA codons cause ribosome stalling to result in the reduction of the encoding protein product. In this study, the additional supply of tRNA Arg (ACG) genes that produce decoding Arg‐tRNA(ICG) promoted the product level from the CGA12‐luc reporter, revealing that the product reduction is essentially due to inefficient decoding and deficiency in the tRNA supply. The mature tRNA Arg (ICG) and the precursor tRNA Arg (ACG) ratios examined for cellular tRNA fraction revealed that the tRNA Arg (ICG) ratio is maintained at less than 30% and is responsive to the Tad2/Tad3 expression level." @default.
• W4321454507 created "2023-02-22" @default.
• W4321454507 creator A5021999767 @default.
• W4321454507 creator A5028206707 @default.
• W4321454507 date "2023-03-06" @default.
• W4321454507 modified "2023-10-14" @default.
• W4321454507 title "The <scp>CGA</scp> codon decoding through <scp>tRNA^Arg</scp>(<scp>ICG</scp>) supply governed by Tad2/Tad3 in <i>Saccharomyces cerevisiae</i>" @default.
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• W4321454507 doi "https://doi.org/10.1111/febs.16760" @default.
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