Matches in SemOpenAlex for { <https://semopenalex.org/work/W4321459303> ?p ?o ?g. }
- W4321459303 endingPage "678" @default.
- W4321459303 startingPage "668" @default.
- W4321459303 abstract "Periodontal ligament stem cells (PDLSCs) are derived from the periodontal ligament and have the characteristics of pluripotent differentiation, including osteogenesis, and are one of the important seed cells in oral tissue engineering. Thyrotropin (TSH) has been shown to regulate bone metabolism independently of thyroid hormone, including the fate of osteoblasts and osteoclasts, but whether it affects osteogenic differentiation of PDLSCs is unknown.PDLSCs were isolated and cultured from human periodontal ligament and grown in osteogenic medium (containing sodium β-glycerophosphate, ascorbic acid, and dexamethasone). Recombinant human TSH was added to the culture medium. Osteogenic differentiation of PDLSCs was assessed after 14 days by staining with alkaline phosphatase and alizarin red and by detection of osteogenic differentiation genes. Differentially expressed genes (DEGs) in PDLSCs under TSH were detected by high-throughput sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyzed the biological functions and signaling pathways involved in DEGs.We found that osteogenic differentiation of PDLSCs was significantly inhibited in the presence of TSH: including decreased calcium nodule formation, decreased alkaline phosphatase levels, and decreased collagen synthesis. Using high-throughput sequencing, we found changes in the expression of some osteogenesis-related genes, which may be the reason that TSH inhibits osteogenic differentiation of PDLSCs.Unless TSH is ≥10 mU/L, patients with subclinical hypothyroidism usually do not undergo thyroxine supplementation therapy. However, in this work, we found that elevated TSH inhibited the osteogenic differentiation of PDLSCs. Therefore, correction of TSH levels in patients with subclinical hypothyroidism may be beneficial to improve orthodontic, implant, and periodontitis outcomes in these patients." @default.
- W4321459303 created "2023-02-22" @default.
- W4321459303 creator A5022249273 @default.
- W4321459303 creator A5023502250 @default.
- W4321459303 creator A5049706981 @default.
- W4321459303 creator A5056694697 @default.
- W4321459303 creator A5066716873 @default.
- W4321459303 creator A5085932220 @default.
- W4321459303 creator A5086326013 @default.
- W4321459303 date "2023-02-20" @default.
- W4321459303 modified "2023-10-18" @default.
- W4321459303 title "Thyrotropin inhibits osteogenic differentiation of human periodontal ligament stem cells" @default.
- W4321459303 cites W1140222300 @default.
- W4321459303 cites W1537337088 @default.
- W4321459303 cites W1596952717 @default.
- W4321459303 cites W1607471435 @default.
- W4321459303 cites W1923465237 @default.
- W4321459303 cites W1990236071 @default.
- W4321459303 cites W2000786270 @default.
- W4321459303 cites W2008514422 @default.
- W4321459303 cites W2014646480 @default.
- W4321459303 cites W2019974313 @default.
- W4321459303 cites W2020114480 @default.
- W4321459303 cites W2035181548 @default.
- W4321459303 cites W2035428418 @default.
- W4321459303 cites W2064524784 @default.
- W4321459303 cites W2077054205 @default.
- W4321459303 cites W2081592691 @default.
- W4321459303 cites W2086476305 @default.
- W4321459303 cites W2090741825 @default.
- W4321459303 cites W2099135651 @default.
- W4321459303 cites W2101928531 @default.
- W4321459303 cites W2108897036 @default.
- W4321459303 cites W2122206763 @default.
- W4321459303 cites W2144224215 @default.
- W4321459303 cites W2147717944 @default.
- W4321459303 cites W2151692383 @default.
- W4321459303 cites W2157932539 @default.
- W4321459303 cites W2163950659 @default.
- W4321459303 cites W2166456614 @default.
- W4321459303 cites W2238461799 @default.
- W4321459303 cites W2260908471 @default.
- W4321459303 cites W2262699654 @default.
- W4321459303 cites W2336959895 @default.
- W4321459303 cites W2530107877 @default.
- W4321459303 cites W2562125986 @default.
- W4321459303 cites W2594066124 @default.
- W4321459303 cites W2761947361 @default.
- W4321459303 cites W2767578741 @default.
- W4321459303 cites W2780025729 @default.
- W4321459303 cites W2790014095 @default.
- W4321459303 cites W2905639264 @default.
- W4321459303 cites W2909878427 @default.
- W4321459303 cites W2917932286 @default.
- W4321459303 cites W2920863879 @default.
- W4321459303 cites W2942046430 @default.
- W4321459303 cites W2952819244 @default.
- W4321459303 cites W2958558042 @default.
- W4321459303 cites W2970121222 @default.
- W4321459303 cites W2974155183 @default.
- W4321459303 cites W2982041070 @default.
- W4321459303 cites W2993524467 @default.
- W4321459303 cites W3005006611 @default.
- W4321459303 cites W3014149864 @default.
- W4321459303 cites W3032045496 @default.
- W4321459303 cites W3045012095 @default.
- W4321459303 cites W3106334216 @default.
- W4321459303 cites W3107816418 @default.
- W4321459303 cites W3184393019 @default.
- W4321459303 cites W3185881618 @default.
- W4321459303 cites W3198486968 @default.
- W4321459303 cites W3199428045 @default.
- W4321459303 cites W3215785807 @default.
- W4321459303 cites W4225698268 @default.
- W4321459303 cites W4233116262 @default.
- W4321459303 cites W4245500425 @default.
- W4321459303 cites W984841677 @default.
- W4321459303 doi "https://doi.org/10.1111/jre.13109" @default.
- W4321459303 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36807238" @default.
- W4321459303 hasPublicationYear "2023" @default.
- W4321459303 type Work @default.
- W4321459303 citedByCount "0" @default.
- W4321459303 crossrefType "journal-article" @default.
- W4321459303 hasAuthorship W4321459303A5022249273 @default.
- W4321459303 hasAuthorship W4321459303A5023502250 @default.
- W4321459303 hasAuthorship W4321459303A5049706981 @default.
- W4321459303 hasAuthorship W4321459303A5056694697 @default.
- W4321459303 hasAuthorship W4321459303A5066716873 @default.
- W4321459303 hasAuthorship W4321459303A5085932220 @default.
- W4321459303 hasAuthorship W4321459303A5086326013 @default.
- W4321459303 hasConcept C104317684 @default.
- W4321459303 hasConcept C126322002 @default.
- W4321459303 hasConcept C134018914 @default.
- W4321459303 hasConcept C148738053 @default.
- W4321459303 hasConcept C150194340 @default.
- W4321459303 hasConcept C152724338 @default.
- W4321459303 hasConcept C160160445 @default.
- W4321459303 hasConcept C162317418 @default.