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- W4321498026 abstract "Dielectric spectroscopy (DS) is the primary technique to observe the dielectric properties of biomaterials. DS extracts complex permittivity spectra from measured frequency responses such as the scattering parameters or impedances of materials over the frequency band of interest. In this study, an open-ended coaxial probe and vector network analyzer were used to characterize the complex permittivity spectra of protein suspensions of human mesenchymal stem cells (hMSCs) and human osteogenic sarcoma (Saos-2) cells in distilled water at frequencies ranging from 10 MHz to 43.5 GHz. The complex permittivity spectra of the protein suspensions of hMSCs and Saos-2 cells revealed two major dielectric dispersions, β and γ, offering three distinctive features for detecting the differentiation of stem cells: the distinctive values in the real and imaginary parts of the complex permittivity spectra as well as the relaxation frequency in the β-dispersion. The protein suspensions were analyzed using a single-shell model, and a dielectrophoresis (DEP) study was performed to determine the relationship between DS and DEP. In immunohistochemistry, antigen–antibody reactions and staining are required to identify the cell type; in contrast, DS eliminates the use of biological processes, while also providing numerical values of the dielectric permittivity of the material-under-test to detect differences. This study suggests that the application of DS can be expanded to detect stem cell differentiation." @default.
- W4321498026 created "2023-02-23" @default.
- W4321498026 creator A5000726733 @default.
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- W4321498026 date "2023-02-21" @default.
- W4321498026 modified "2023-10-14" @default.
- W4321498026 title "Novel Sensing Technique for Stem Cells Differentiation Using Dielectric Spectroscopy of Their Proteins" @default.
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- W4321498026 doi "https://doi.org/10.3390/s23052397" @default.
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