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- W4321509124 abstract "Abstract Serologic ABO blood typing is routinely performed using anti-A and anti-B sera to distinguish four phenotypes (A, B, AB, and O). Restriction fragment length polymorphisms (RFLPs) and DNA sequence studies offer the possibility of direct ABO genotyping. We used polymerase chain reaction-RFLP analysis to determine the frequency of O 1 and O 2 alleles in 82 unrelated blood donors in São Paulo, Brazil, known to be group O. Genomic DNA was extracted from blood leukocytes by a modified salting-out method. Different genotypes (O 1 O 1 , O 1 O 2 , O 2 O 2 ) were identified after digestion with restriction enzymes Kpn I, Hpa II, and Alu I, followed by agarose gel electrophoresis. Of 82 samples analyzed, 74 were O 1 O 1 , 7 were O 1 O 2 , and 1 was O 2 O 2 . These results showed the frequency of O 1 O 1 , O 1 O 2 , and O 2 O 2 genotypes to be 90.24 percent, 8.53 percent, and 1.22 percent, respectively, in blood donors in São Paulo, Brazil. Immunohematology 2001;17:111–116 ." @default.
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- W4321509124 date "2001-01-01" @default.
- W4321509124 modified "2023-09-27" @default.
- W4321509124 title "Easy method for determining the frequency of <i>O<sup>1</sup> </i> and <i>O<sup>2</sup> </i> alleles in Brazilian blood donors by PCR-RFLP analysis" @default.
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- W4321509124 doi "https://doi.org/10.21307/immunohematology-2019-563" @default.
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