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- W432152846 abstract "We studied the process of transcription of T4 early genes by analyzing the synthesis of T4-induced proteins by gel electrophoresis. The simultaneous addition of the antibiotic rifampicin and a 3H-labeled amino acids mixture to infected cells at various times allowed us to estimate the amounts of mRNAs for T4 early genes formed during the course of infection, assuming that mRNAs are translated at a constant rate. According to the patterns of transcriptional activity, we classified T4 early genes as follows. Class I (genes 39, rIIA, 42, y, and A6–1); the transcription takes place immediately and ceases within 1 min after phage infection. Class II (genes 43, 46, and rIIB); there are significant levels of transcription throughout the process (two peaks of activity are usually observed). Class III (gene 52); an initial high level of transcription is followed by a slow decline and a second transcriptional peak at the later stage (10–13 min after infection). Class IV (gene 32); the transcriptional level increases gradually and ceases at the later stage (12 min after infection). On infection with ultraviolet-irradiated phage, significantly altered patterns of transcription were observed. The levels of transcriptional activity for most genes increased continuously. In addition, it was found that the initiation of transcription of some of the genes was delayed (no transcription of genes 43 and 46 took place within 5 min after infection). Based on these results, we proposed that T4 early genes are controlled by at least four classes of promotors." @default.
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- W432152846 date "1978-10-01" @default.
- W432152846 modified "2023-09-23" @default.
- W432152846 title "The Expression of Bacteriophage T4 Early Genes" @default.
- W432152846 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a132198" @default.
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