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- W4322217263 abstract "Here, we detail the immunization of mice with a sublethal dose of MERS-CoV or two doses of replication-incompetent alphavirus replicon particles expressing MERS-CoV spike protein. We then describe steps to determine the outcome of immunization by challenging immunized mice with a lethal dose of MERS-CoV, as well as by detecting virus-specific neutralizing antibody and virus-specific T cell response via neutralization assay and flow cytometry, respectively. This protocol can be used to evaluate other CoV infections or vaccine-induced immune responses. For complete details on the use and execution of this protocol, please refer to Zheng et al. (2021).1." @default.
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- W4322217263 date "2023-06-01" @default.
- W4322217263 modified "2023-09-30" @default.
- W4322217263 title "Immunization of MERS-CoV-2-infected mice with a sublethal dose of MERS-CoV or VRP-MERS-S" @default.
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- W4322217263 doi "https://doi.org/10.1016/j.xpro.2023.102171" @default.
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