FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4322388503> ?p ?o ?g. }

• W4322388503 endingPage "73" @default.
• W4322388503 startingPage "62" @default.
• W4322388503 abstract "Human CD34+ hematopoietic progenitor cells obtained from bone marrow (BM), umbilical cord blood (UCB), and mobilized peripheral blood (MPB) were purified and investigated for the expression of the chemokine receptor CXCR4 and its ligand, stromal cell–derived factor-1 (SDF-1). CXCR4 was found present on the cell surface of all CD34+ cells, although it was expressed at lower density on MPB with respect to BM CD34+ cells. Freshly isolated and in vitro–cultured CD34+ cells also coexpressed SDF-1 mRNA, as determined by reverse transcriptase-polymerase chain reaction (RT-PCR). Of interest, CD34+/CD38+ committed progenitor cells, unlike primitive CD34+/CD38− cells, expressed SDF-1 mRNA. Supernatants from in vitro–cultured CD34+ cells contained substantial (3 to 8 ng/mL) amounts of SDF-1 by enzyme-linked immunosorbent assay and induced migration of CD34+ cells. Because CD34+ cells express low levels of CD4, the primary receptor of the human immunodeficiency virus (HIV), and CXCR4 is a coreceptor for T-cell tropic (X4) HIV strains, we investigated the susceptibility of CD34+cells to infection by this subset of viruses. Lack of productive infection was almost invariably observed as determined by a conventional RT activity in culture supernatants and by real-time PCR for HIV DNA in CD34+ cells exposed to both laboratory adapted (LAI) and primary (BON) X4 T-cell tropic HIV-1 strain. Soluble gp120 Env (sgp120) from X4 HIV-1 efficiently blocked binding of the anti-CD4 Leu3a monoclonal antibody (MoAb) to either human CD4+ T cells or CD34+ cells. In contrast, sgp120 interfered with an anti-CXCR4 MoAb binding to human T lymphocytes, but not to CD34+ cells. However, CXCR4 on CD34+ cells was downregulated by SDF-1. These results suggest that CXCR4 and its ligand SDF-1 expressed in CD34+ progenitors may play an important role in regulating the local and systemic trafficking of these cells. Moreover, these findings suggest multiple and potentially synergistic mechanisms at the basis of the resistance of CD34+ cells to X4 HIV infection, including their ability to produce SDF-1, and the lack of CXCR4 internalization following gp120 binding to CD4." @default.
• W4322388503 created "2023-02-27" @default.
• W4322388503 creator A5019228154 @default.
• W4322388503 creator A5021416524 @default.
• W4322388503 creator A5024849307 @default.
• W4322388503 creator A5030216074 @default.
• W4322388503 creator A5044577073 @default.
• W4322388503 creator A5049567451 @default.
• W4322388503 creator A5056887177 @default.
• W4322388503 creator A5062067515 @default.
• W4322388503 creator A5078160189 @default.
• W4322388503 creator A5080534919 @default.
• W4322388503 date "1999-07-01" @default.
• W4322388503 modified "2023-10-11" @default.
• W4322388503 title "Human CD34+ Cells Express CXCR4 and Its Ligand Stromal Cell–Derived Factor-1. Implications for Infection by T-Cell Tropic Human Immunodeficiency Virus" @default.
• W4322388503 cites W1486525240 @default.
• W4322388503 cites W1554718783 @default.
• W4322388503 cites W1566936977 @default.
• W4322388503 cites W1592374520 @default.
• W4322388503 cites W1623426438 @default.
• W4322388503 cites W1752108741 @default.
• W4322388503 cites W1757653714 @default.
• W4322388503 cites W1963984954 @default.
• W4322388503 cites W1964083898 @default.
• W4322388503 cites W1974132609 @default.
• W4322388503 cites W1978136857 @default.
• W4322388503 cites W1979989842 @default.
• W4322388503 cites W1981440980 @default.
• W4322388503 cites W1983466424 @default.
• W4322388503 cites W1989035987 @default.
• W4322388503 cites W1998428445 @default.
• W4322388503 cites W2003856370 @default.
• W4322388503 cites W2004216239 @default.
• W4322388503 cites W2008879260 @default.
• W4322388503 cites W2022227920 @default.
• W4322388503 cites W2040827928 @default.
• W4322388503 cites W2053606105 @default.
• W4322388503 cites W2053704719 @default.
• W4322388503 cites W2059626630 @default.
• W4322388503 cites W2060686995 @default.
• W4322388503 cites W2061285112 @default.
• W4322388503 cites W2062148688 @default.
• W4322388503 cites W2070721758 @default.
• W4322388503 cites W2072286695 @default.
• W4322388503 cites W2080350299 @default.
• W4322388503 cites W2082925733 @default.
• W4322388503 cites W2087919417 @default.
• W4322388503 cites W2091092355 @default.
• W4322388503 cites W2106915606 @default.
• W4322388503 cites W2113112343 @default.
• W4322388503 cites W2114668630 @default.
• W4322388503 cites W2120051788 @default.
• W4322388503 cites W2129664184 @default.
• W4322388503 cites W2138562527 @default.
• W4322388503 cites W2158294445 @default.
• W4322388503 cites W2160224680 @default.
• W4322388503 cites W2164578725 @default.
• W4322388503 cites W2165049218 @default.
• W4322388503 cites W2165244186 @default.
• W4322388503 cites W2166240517 @default.
• W4322388503 cites W2167037373 @default.
• W4322388503 cites W2169584423 @default.
• W4322388503 cites W2171248814 @default.
• W4322388503 cites W2176220887 @default.
• W4322388503 cites W2258378031 @default.
• W4322388503 cites W2258659648 @default.
• W4322388503 cites W226476106 @default.
• W4322388503 cites W2264796473 @default.
• W4322388503 cites W2281127542 @default.
• W4322388503 cites W2289017011 @default.
• W4322388503 cites W2299362120 @default.
• W4322388503 cites W2308127601 @default.
• W4322388503 cites W2311232153 @default.
• W4322388503 cites W237249694 @default.
• W4322388503 cites W2394720881 @default.
• W4322388503 cites W2396040074 @default.
• W4322388503 cites W2398146302 @default.
• W4322388503 cites W2406664546 @default.
• W4322388503 cites W4211264883 @default.
• W4322388503 cites W551869 @default.
• W4322388503 cites W597759872 @default.
• W4322388503 cites W83564348 @default.
• W4322388503 cites W88241752 @default.
• W4322388503 doi "https://doi.org/10.1182/blood.v94.1.62.413k04_62_73" @default.
• W4322388503 hasPublicationYear "1999" @default.
• W4322388503 type Work @default.
• W4322388503 citedByCount "98" @default.
• W4322388503 countsByYear W43223885032012 @default.
• W4322388503 countsByYear W43223885032014 @default.
• W4322388503 countsByYear W43223885032015 @default.
• W4322388503 countsByYear W43223885032016 @default.
• W4322388503 countsByYear W43223885032017 @default.
• W4322388503 countsByYear W43223885032018 @default.
• W4322388503 countsByYear W43223885032020 @default.
• W4322388503 countsByYear W43223885032022 @default.
• W4322388503 crossrefType "journal-article" @default.
• W4322388503 hasAuthorship W4322388503A5019228154 @default.
• W4322388503 hasAuthorship W4322388503A5021416524 @default.

• next